Volume 272, Number 21, Issue of May 23, 1997 pp. 13459-13462
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

COMMUNICATION:
Evidence for CPP32 Activation in the Absence of Apoptosis during T Lymphocyte Stimulation

(Received for publication, March 7, 1997)

From Roussel Uclaf, 102 route de Noisy, 93235 Romainville Cedex, France

Cysteine proteases of the interleukin-1-converting enzyme family have been implicated in the effector process of apoptosis in several systems. Among these, CPP32 has been shown to be processed to active enzyme at the onset of apoptosis. Here, we show that CPP32 precursor is cleaved into its active form during phytohaemaglutinin A activation of T lymphocytes. Maximal processing is observed between day 3 and day 4 following addition of mitogen and is a transient process. Precursor cleavage is associated with the appearance of a CPP32-like enzymatic activity in cell lysates. At this time in the culture, almost no apoptotic cell and no dead cell can be detected, and T lymphocytes are actively proliferating. CPP32 processing also occurs when lymphocytes are stimulated through an allogeneic primary mixed lymphocyte reaction. Our results suggest that proteolytic activation of CPP32 could be a physiological step during T lymphocyte activation. In addition, these data indicate that CPP32 activation can occur independently of programmed cell death in T lymphocytes.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Truscott, J.-B. Denault, B. Goulet, L. Leduy, G. S. Salvesen, and A. Nepveu Carboxyl-terminal Proteolytic Processing of CUX1 by a Caspase Enables Transcriptional Activation in Proliferating Cells J. Biol. Chem., October 12, 2007; 282(41): 30216 - 30226. [Abstract] [Full Text] [PDF]

				B. Lemmers, L. Salmena, N. Bidere, H. Su, E. Matysiak-Zablocki, K. Murakami, P. S. Ohashi, A. Jurisicova, M. Lenardo, R. Hakem, et al. Essential Role for Caspase-8 in Toll-like Receptors and NF{kappa}B Signaling J. Biol. Chem., March 9, 2007; 282(10): 7416 - 7423. [Abstract] [Full Text] [PDF]

				K. J. Rautajoki, E. M. Marttila, T. A. Nyman, and R. Lahesmaa Interleukin-4 Inhibits Caspase-3 by Regulating Several Proteins in the Fas Pathway during Initial Stages of Human T Helper 2 Cell Differentiation Mol. Cell. Proteomics, February 1, 2007; 6(2): 238 - 251. [Abstract] [Full Text] [PDF]

				J. W. Darnowski, F. A. Goulette, Y.-j. Guan, D. Chatterjee, Z.-F. Yang, L. P. Cousens, and Y. E. Chin Stat3 Cleavage by Caspases: IMPACT ON FULL-LENGTH Stat3 EXPRESSION, FRAGMENT FORMATION, AND TRANSCRIPTIONAL ACTIVITY J. Biol. Chem., June 30, 2006; 281(26): 17707 - 17717. [Abstract] [Full Text] [PDF]

				M. Maksimow, T. S. Soderstrom, S. Jalkanen, J. E. Eriksson, and A. Hanninen Fas costimulation of naive CD4 T cells is controlled by NF-{kappa}B signaling and caspase activity J. Leukoc. Biol., February 1, 2006; 79(2): 369 - 377. [Abstract] [Full Text] [PDF]

				I. Decordier, E. Cundari, and M. Kirsch-Volders Influence of caspase activity on micronuclei detection: a possible role for caspase-3 in micronucleation Mutagenesis, May 1, 2005; 20(3): 173 - 179. [Abstract] [Full Text] [PDF]

				A. N.M. Fischer, B. Herrera, M. Mikula, V. Proell, E. Fuchs, J. Gotzmann, R. Schulte-Hermann, H. Beug, and W. Mikulits Integration of Ras subeffector signaling in TGF-{beta} mediated late stage hepatocarcinogenesis Carcinogenesis, May 1, 2005; 26(5): 931 - 942. [Abstract] [Full Text] [PDF]

				L. Sabbagh, S. M. Kaech, M. Bourbonniere, M. Woo, L. Y. Cohen, E. K. Haddad, N. Labrecque, R. Ahmed, and R.-P. Sekaly The Selective Increase in Caspase-3 Expression in Effector but Not Memory T Cells Allows Susceptibility to Apoptosis J. Immunol., November 1, 2004; 173(9): 5425 - 5433. [Abstract] [Full Text] [PDF]

				M. Falk, S. Ussat, N. Reiling, D. Wesch, D. Kabelitz, and S. Adam-Klages Caspase Inhibition Blocks Human T Cell Proliferation by Suppressing Appropriate Regulation of IL-2, CD25, and Cell Cycle-Associated Proteins J. Immunol., October 15, 2004; 173(8): 5077 - 5085. [Abstract] [Full Text] [PDF]

				E. Dondi, G. Roue, V. J. Yuste, S. A. Susin, and S. Pellegrini A Dual Role of IFN-{alpha} in the Balance between Proliferation and Death of Human CD4+ T Lymphocytes during Primary Response J. Immunol., September 15, 2004; 173(6): 3740 - 3747. [Abstract] [Full Text] [PDF]

				R. Song, R. S. Mahidhara, Z. Zhou, R. A. Hoffman, D.-W. Seol, R. A. Flavell, T. R. Billiar, L. E. Otterbein, and A. M. K. Choi Carbon Monoxide Inhibits T Lymphocyte Proliferation via Caspase-Dependent Pathway J. Immunol., January 15, 2004; 172(2): 1220 - 1226. [Abstract] [Full Text] [PDF]

				R. S. Mahidhara, R. A. Hoffman, S. Huang, A. Wolf-Johnston, Y. Vodovotz, R. L. Simmons, and T. R. Billiar Nitric oxide-mediated inhibition of caspase-dependent T lymphocyte proliferation J. Leukoc. Biol., September 1, 2003; 74(3): 403 - 411. [Abstract] [Full Text] [PDF]

				G. Strauss, I. Knape, I. Melzner, and K.-M. Debatin Constitutive Caspase Activation and Impaired Death-Inducing Signaling Complex Formation in CD95-Resistant, Long-Term Activated, Antigen-Specific T Cells J. Immunol., August 1, 2003; 171(3): 1172 - 1182. [Abstract] [Full Text] [PDF]

				T. Kaji, S. Hachimura, W. Ise, and S. Kaminogawa Proteome Analysis Reveals Caspase Activation in Hyporesponsive CD4 T Lymphocytes Induced in Vivo by the Oral Administration of Antigen J. Biol. Chem., July 18, 2003; 278(30): 27836 - 27843. [Abstract] [Full Text] [PDF]

				S. Faderl, D. Harris, Q. Van, H. M. Kantarjian, M. Talpaz, and Z. Estrov Granulocyte-macrophage colony-stimulating factor (GM-CSF) induces antiapoptotic and proapoptotic signals in acute myeloid leukemia Blood, July 15, 2003; 102(2): 630 - 637. [Abstract] [Full Text] [PDF]

				R. M. O'Neill, J. Hassan, and D. J. Reen IL-7-Regulated Homeostatic Maintenance of Recent Thymic Emigrants in Association with Caspase-Mediated Cell Proliferation and Apoptotic Cell Death J. Immunol., May 1, 2003; 170(9): 4524 - 4531. [Abstract] [Full Text] [PDF]

				A. D. Chernysheva, K. A. Kirou, and M. K. Crow T Cell Proliferation Induced by Autologous Non-T Cells Is a Response to Apoptotic Cells Processed by Dendritic Cells J. Immunol., August 1, 2002; 169(3): 1241 - 1250. [Abstract] [Full Text] [PDF]

				V. Frost, S. Delikat, S. Al-Mehairi, and A. J. Sinclair Regulation of p27KIP1 in Epstein-Barr virus-immortalized lymphoblastoid cell lines involves non-apoptotic caspase cleavage J. Gen. Virol., December 1, 2001; 82(12): 3057 - 3066. [Abstract] [Full Text] [PDF]

				M. P. CATALAN, A. REYERO, J. EGIDO, and A. ORTIZ Acceleration of Neutrophil Apoptosis by Glucose-Containing Peritoneal Dialysis Solutions: Role of Caspases J. Am. Soc. Nephrol., November 1, 2001; 12(11): 2442 - 2449. [Abstract] [Full Text] [PDF]

				J. Zhang, K. Mikecz, A. Finnegan, and T. T. Glant Spontaneous Thymocyte Apoptosis Is Regulated by a Mitochondrion-Mediated Signaling Pathway J. Immunol., September 15, 2000; 165(6): 2970 - 2974. [Abstract] [Full Text] [PDF]

				S. F. Law, G. M. O'Neill, S. J. Fashena, M. B. Einarson, and E. A. Golemis The Docking Protein HEF1 Is an Apoptotic Mediator at Focal Adhesion Sites Mol. Cell. Biol., July 15, 2000; 20(14): 5184 - 5195. [Abstract] [Full Text]

				K. B. Elkon Caspases: Multifunctional Proteases J. Exp. Med., December 20, 1999; 190(12): 1725 - 1728. [Full Text] [PDF]

				A. Alam, L. Y. Cohen, S. Aouad, and R.-P. Sekaly Early Activation of Caspases during T Lymphocyte Stimulation Results in Selective Substrate Cleavage in Nonapoptotic Cells J. Exp. Med., December 20, 1999; 190(12): 1879 - 1890. [Abstract] [Full Text] [PDF]

				B. FADEEL, B. ZHIVOTOVSKY, and S. ORRENIUS All along the watchtower: on the regulation of apoptosis regulators FASEB J, October 1, 1999; 13(13): 1647 - 1657. [Abstract] [Full Text]

				A. Shcherbina and E. Remold-O'Donnell Role of Caspase in a Subset of Human Platelet Activation Responses Blood, June 15, 1999; 93(12): 4222 - 4231. [Abstract] [Full Text] [PDF]

				P. Saas, J. Boucraut, A.-L. Quiquerez, V. Schnuriger, G. Perrin, S. Desplat-Jego, D. Bernard, P. R. Walker, and P.-Y. Dietrich CD95 (Fas/Apo-1) as a Receptor Governing Astrocyte Apoptotic or Inflammatory Responses: A Key Role in Brain Inflammation? J. Immunol., February 15, 1999; 162(4): 2326 - 2333. [Abstract] [Full Text] [PDF]

				P. Vito, L. Pellegrini, C. Guiet, and L. D'Adamio Cloning of AIP1, a Novel Protein That Associates with the Apoptosis-linked Gene ALG-2 in a Ca2+-dependent Reaction J. Biol. Chem., January 15, 1999; 274(3): 1533 - 1540. [Abstract] [Full Text] [PDF]

				L. Stefanis, C. M. Troy, H. Qi, M. L. Shelanski, and L. A. Greene Caspase-2 (Nedd-2) Processing and Death of Trophic Factor-Deprived PC12 Cells and Sympathetic Neurons Occur Independently of Caspase-3 (CPP32)-Like Activity J. Neurosci., November 15, 1998; 18(22): 9204 - 9215. [Abstract] [Full Text] [PDF]

				T. Hirsch, B. Dallaporta, N. Zamzami, S. A. Susin, L. Ravagnan, I. Marzo, C. Brenner, and G. Kroemer Proteasome Activation Occurs at an Early, Premitochondrial Step of Thymocyte Apoptosis J. Immunol., July 1, 1998; 161(1): 35 - 40. [Abstract] [Full Text] [PDF]

				C.-S. Park, Y. Yashiro, X.-G. Tai, K. Toyo-oka, T. Hamaoka, H. Yagita, K. Okumura, S. Neben, and H. Fujiwara Differential Involvement of a Fas-CPP32-Like Protease Pathway in Apoptosis of TCR/CD9-Costimulated, Naive T Cells and TCR-Restimulated, Activated T Cells J. Immunol., June 15, 1998; 160(12): 5790 - 5796. [Abstract] [Full Text] [PDF]

				V. Cryns and J. Yuan Proteases to die for Genes & Dev., June 1, 1998; 12(11): 1551 - 1570. [Full Text]

				J. M. Zapata, R. Takahashi, G. S. Salvesen, and J. C. Reed Granzyme Release and Caspase Activation in Activated Human T-Lymphocytes J. Biol. Chem., March 20, 1998; 273(12): 6916 - 6920. [Abstract] [Full Text] [PDF]

				S. C. Wright, U. Schellenberger, H. Wang, D. H. Kinder, J. W. Talhouk, and J. W. Larrick Activation of CPP32-like Proteases Is Not Sufficient to Trigger Apoptosis: Inhibition of Apoptosis by Agents that Suppress Activation of AP24, but Not CPP32-like Activity J. Exp. Med., October 6, 1997; 186(7): 1107 - 1117. [Abstract] [Full Text] [PDF]

				C. Caulin, G. S. Salvesen, and R. G. Oshima Caspase Cleavage of Keratin 18 and Reorganization of Intermediate Filaments during Epithelial Cell Apoptosis J. Cell Biol., September 22, 1997; 138(6): 1379 - 1394. [Abstract] [Full Text] [PDF]