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Volume 272, Number 21, Issue of May 23, 1997 pp. 13660-13665
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

SecE-depleted Membranes of Escherichia coli Are Active
SecE IS NOT OBLIGATORILY REQUIRED FOR THE IN VITRO TRANSLOCATION OF CERTAIN PROTEIN PRECURSORS

(Received for publication, December 3, 1996, and in revised form, February 3, 1997)

Yunn-Bor Yang , Nianjun Yu and Phang C. Tai

From the Department of Biology, Georgia State University, Atlanta, Georgia 30303

Membrane vesicles were prepared from Escherichia coli cells in which SecE was depleted to 2% of wild-type membranes. SecE depletion had pleiotropic effects; SecD, SecF, SecG, and SecY were decreased 4-6-fold, whereas SecA was increased about 16-fold over that of wild-type membranes. These membranes were substantially active in the in vitro translocation of proOmpA, which was mediated by the SecA pathway since it was inhibited by azide. Similar substantial translocation activities were observed for proLamB and proLpp in the SecE-depleted membranes. However, the translocation of proPhoA was more severely impaired. These data indicate that SecE may enhance but is not obligatorily required for the translocation of at least certain precursors, and suggest that the effects of the SecE depletion on protein translocation may be precursor-dependent.


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