|
|
|
|||||||
|
|||||||||
(Received for publication, May 13, 1996, and in revised form, February 18, 1997)
From the Departments of Medicine and Cell Biology, Division of
Endocrinology and Metabolism, The University of Alabama at
Birmingham, Birmingham, Alabama 35294
The epidermal growth factor receptor is vital for
normal development and plays a role in oncogenesis. The level of
activation of this receptor by transforming growth factor-
Volume 272, Number 22,
Issue of May 30, 1997
pp. 14244-14250
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
Gene
(TGF-)
is controlled, in part, by the rate of transcription of the TGF-
gene. In the characterization of the proximal TGF- promoter by DNase
I footprinting, a 43-base pair element (
88 to 130 relative to the
transcription start site), designated TRE I, was found that was
specifically protected by nuclear proteins from human mammary carcinoma
MDA468 cells. TRE I was essential for the maximal expression of the TGF- gene as indicated by deletion and mutagenesis analyses. TRE
I consists of two cis-acting elements, a proximal
regulatory element (PRE, 89 to 103) and a distal regulatory element
(DRE, 121 to 128). Both elements were able to form specific
complexes with protein from MDA468 cell nuclear extracts and are
necessary for the full activity of the entire 1.1-kilobase pair TGF-
promoter. Competition and antibody studies determined that the DRE
contains a binding site for the transcription factor AP-2, while the
protein that binds to the PRE has yet to be identified. When linked
upstream to the heterologous herpes simplex thymidine kinase promoter, the TRE I enhanced transcription up to 11-fold in MDA468 cells. Cotransfection of an AP-2 expression vector was able to activate transcription from the TREI-TK construct in a
DRE-dependent manner. These results further our
understanding of how TGF- transcription is regulated.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kondo, H. Maegawa, T. Obata, S. Ugi, K. Ikeda, K. Morino, Y. Nakai, Y. Nishio, S. Maeda, and A. Kashiwagi Transcription Factor Activating Protein-2{beta}: A Positive Regulator of Monocyte Chemoattractant Protein-1 Gene Expression Endocrinology, April 1, 2009; 150(4): 1654 - 1661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-S. Byun, G.-S. Lee, B.-M. Lee, S.-H. Hyun, K.-C. Choi, and E.-B. Jeung Implantation-Related Expression of Epidermal Growth Factor Family Molecules and Their Regulation by Progesterone in the Pregnant Rat Reproductive Sciences, September 1, 2008; 15(7): 678 - 689. [Abstract] [PDF]
|
|
|
|
 |
|
 F. Orso, E. Penna, D. Cimino, E. Astanina, F. Maione, D. Valdembri, E. Giraudo, G. Serini, P. Sismondi, M. De Bortoli, et al. AP-2{alpha} and AP-2{gamma} regulate tumor progression via specific genetic programs FASEB J, August 1, 2008; 22(8): 2702 - 2714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Dhakshinamoorthy, S. R. Sridharan, L. Li, P. Y. Ng, L. M. Boxer, and A. G. Porter Protein/DNA arrays identify nitric oxide-regulated cis-element and trans-factor activities some of which govern neuroblastoma cell viability Nucleic Acids Res., August 15, 2007; (2007) gkm594v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y Sato, J Kato, R Takimoto, K Takada, Y Kawano, K Miyanishi, M Kobune, Y Sato, T Takayama, T Matunaga, et al. Hepatitis C virus core protein promotes proliferation of human hepatoma cells through enhancement of transforming growth factor {alpha} expression via activation of nuclear factor-{kappa}B Gut, December 1, 2006; 55(12): 1801 - 1808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ikeda, H. Maegawa, S. Ugi, Y. Tao, Y. Nishio, S. Tsukada, S. Maeda, and A. Kashiwagi Transcription Factor Activating Enhancer-binding Protein-2beta: A NEGATIVE REGULATOR OF ADIPONECTIN GENE EXPRESSION J. Biol. Chem., October 20, 2006; 281(42): 31245 - 31253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wang, D. Bolotin, D. H. Chu, L. Polak, T. Williams, and E. Fuchs AP-2{alpha}: a regulator of EGF receptor signaling and proliferation in skin epidermis J. Cell Biol., January 30, 2006; 172(3): 409 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Mohamed, T. Hanke, C. Schlueter, J. Bullerdiek, and H.-H. Sievers Ubiquitin fusion degradation 1-like gene dysregulation in bicuspid aortic valve J. Thorac. Cardiovasc. Surg., December 1, 2005; 130(6): 1531 - 1536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tellez, M. McCarty, M. Ruiz, and M. Bar-Eli Loss of Activator Protein-2{alpha} Results in Overexpression of Protease-activated Receptor-1 and Correlates with the Malignant Phenotype of Human Melanoma J. Biol. Chem., November 21, 2003; 278(47): 46632 - 46642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Kwon, S. Keates, S. Simeonidis, F. Grall, T. A. Libermann, and A. C. Keates ESE-1, an Enterocyte-specific Ets Transcription Factor, Regulates MIP-3alpha Gene Expression in Caco-2 Human Colonic Epithelial Cells J. Biol. Chem., January 3, 2003; 278(2): 875 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Braganca, T. Swingler, F. I. R. Marques, T. Jones, J. J. Eloranta, H. C. Hurst, T. Shioda, and S. Bhattacharya Human CREB-binding Protein/p300-interacting Transactivator with ED-rich Tail (CITED) 4, a New Member of the CITED Family, Functions as a Co-activator for Transcription Factor AP-2 J. Biol. Chem., March 1, 2002; 277(10): 8559 - 8565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ruiz, P. Troncoso, C. Bruns, and M. Bar-Eli Activator Protein 2{alpha} Transcription Factor Expression Is Associated with Luminal Differentiation and Is Lost in Prostate Cancer Clin. Cancer Res., December 1, 2001; 7(12): 4086 - 4095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Keates, S. Keates, J. H. Kwon, K. O. Arseneau, D. J. Law, L. Bai, J. L. Merchant, T. C. Wang, and C. P. Kelly ZBP-89, Sp1, and Nuclear Factor-kappa B Regulate Epithelial Neutrophil-activating Peptide-78 Gene Expression in Caco-2 Human Colonic Epithelial Cells J. Biol. Chem., November 16, 2001; 276(47): 43713 - 43722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Smith, E. R. Fedyk, T. A. Springer, N. Mukaida, B. H. Iglewski, and R. P. Phipps IL-8 Production in Human Lung Fibroblasts and Epithelial Cells Activated by the Pseudomonas Autoinducer N-3-Oxododecanoyl Homoserine Lactone Is Transcriptionally Regulated by NF-{{kappa}}B and Activator Protein-2 J. Immunol., July 1, 2001; 167(1): 366 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Miettinen, M Huotari, T Koivisto, J Ustinov, J Palgi, S Rasilainen, E Lehtonen, J Keski-Oja, and T Otonkoski Impaired migration and delayed differentiation of pancreatic islet cells in mice lacking EGF-receptors Development, January 6, 2000; 127(12): 2617 - 2627. [Abstract] [PDF]
|
|
|
|
|
|
H. Qin, Y. Sun, and E. N. Benveniste The Transcription Factors Sp1, Sp3, and AP-2 Are Required for Constitutive Matrix Metalloproteinase-2 Gene Expression in Astroglioma Cells J. Biol. Chem., October 8, 1999; 274(41): 29130 - 29137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Imhof, M. Schuierer, O. Werner, M. Moser, C. Roth, R. Bauer, and R. Buettner Transcriptional Regulation of the AP-2alpha Promoter by BTEB-1 and AP-2rep, a Novel wt-1/egr-Related Zinc Finger Repressor Mol. Cell. Biol., January 1, 1999; 19(1): 194 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Shi and R. E. Kellems Transcription Factor AP-2gamma Regulates Murine Adenosine Deaminase Gene Expression during Placental Development J. Biol. Chem., October 16, 1998; 273(42): 27331 - 27338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wankhade, Y. Yu, J. Weinberg, M. A. Tainsky, and P. Kannan Characterization of the Activation Domains of AP-2 Family Transcription Factors J. Biol. Chem., September 15, 2000; 275(38): 29701 - 29708. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |