| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, September 27, 1996, and in revised form, March 26, 1997)
From the Joint Program in Neonatology, Harvard Medical School,
Boston, Massachusetts 02115
The syndecan family of transmembrane heparan
sulfate proteoglycans is abundant on the surface of all adherent
mammalian cells. Syndecans bind and modify the action of various growth
factors/cytokines, proteases/antiproteases, cell adhesion molecules,
and extracellular matrix components. Syndecan expression is highly
regulated during wound repair, a process orchestrated by many of these
effectors. Each syndecan ectodomain is shed constitutively by cultured
cells, but the mechanism and significance of this shedding are not
understood. Therefore, we examined (i) whether physiological agents
active during wound repair influence syndecan shedding, and (ii)
whether wound fluids contain shed syndecan ectodomains.
Using SVEC4-10 endothelial cells we find that certain proteases and
growth factors accelerate shedding of the syndecan-1 and -4 ectodomains. Protease-accelerated shedding is completely inhibited by
serum-containing media. Thrombin activity is duplicated by the 14-amino
acid thrombin receptor agonist peptide that directly activates the
thrombin receptor and is not inhibited by serum. Epidermal growth
factor family members accelerate shedding but FGF-2, platelet-derived
growth factor-AB, transforming growth factor-
Volume 272, Number 23,
Issue of June 6, 1997
pp. 14713-14720
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
, tumor necrosis
factor-
, and vascular endothelial cell growth factor 165 do not.
Shed ectodomains are soluble, stable in the conditioned medium, have
the same size core proteins regardless whether shed at a basal rate, or
accelerated by thrombin or epidermal growth factor-family members and
are found in acute human dermal wound fluids. Thus, shedding is
accelerated by activation of at least two distinct receptor classes, G
protein-coupled (thrombin) and protein tyrosine kinase (epidermal
growth factor). Proteases and growth factors active during wound repair
can accelerate syndecan shedding from cell surfaces. Regulated shedding
of syndecans suggests physiological roles for the soluble proteoglycan
ectodomains.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Zhang, B. D. Humphreys, and J. V. Bonventre Shedding of the Urinary Biomarker Kidney Injury Molecule-1 (KIM-1) Is Regulated by MAP Kinases and Juxtamembrane Region J. Am. Soc. Nephrol., October 1, 2007; 18(10): 2704 - 2714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Su, S. A. Blaine, D. Qiao, and A. Friedl Shedding of Syndecan-1 by Stromal Fibroblasts Stimulates Human Breast Cancer Cell Proliferation via FGF2 Activation J. Biol. Chem., May 18, 2007; 282(20): 14906 - 14915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, A. Hayashida, A. E. Bennett, S. K. Hollingshead, and P. W. Park Streptococcus pneumoniae Sheds Syndecan-1 Ectodomains through ZmpC, a Metalloproteinase Virulence Factor J. Biol. Chem., January 5, 2007; 282(1): 159 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Julien, P. Wang, C. A. Haller, J. Wen, and E. L. Chaikof Mechanical strain regulates syndecan-4 expression and shedding in smooth muscle cells through differential activation of MAP kinase signaling pathways Am J Physiol Cell Physiol, January 1, 2007; 292(1): C517 - C525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Leiner, D. Newman, C.-M. Li, E. Walsh, J. Khosla, and P. L. Sannes Heparin and Fibroblast Growth Factors Affect Surfactant Protein Gene Expression in Type II Cells Am. J. Respir. Cell Mol. Biol., November 1, 2006; 35(5): 611 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-C. Chung, T. G. Popova, B. A. Millis, D. V. Mukherjee, W. Zhou, L. A. Liotta, E. F. Petricoin, V. Chandhoke, C. Bailey, and S. G. Popov Secreted Neutral Metalloproteases of Bacillus anthracis as Candidate Pathogenic Factors J. Biol. Chem., October 20, 2006; 281(42): 31408 - 31418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koch, G. Veit, S. Stricker, P. Bhatt, S. Kutsch, P. Zhou, E. Reinders, R. A. Hahn, R. Song, R. E. Burgeson, et al. Expression of Type XXIII Collagen mRNA and Protein J. Biol. Chem., July 28, 2006; 281(30): 21546 - 21557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Brule, N. Charnaux, A. Sutton, D. Ledoux, T. Chaigneau, L. Saffar, and L. Gattegno The shedding of syndecan-4 and syndecan-1 from HeLa cells and human primary macrophages is accelerated by SDF-1/CXCL12 and mediated by the matrix metalloproteinase-9 Glycobiology, June 1, 2006; 16(6): 488 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Fears, C. L. Gladson, and A. Woods Syndecan-2 Is Expressed in the Microvasculature of Gliomas and Regulates Angiogenic Processes in Microvascular Endothelial Cells J. Biol. Chem., May 26, 2006; 281(21): 14533 - 14536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Selleck Shedding Light on the Distinct Functions of Proteoglycans Sci. Signal., April 4, 2006; 2006(329): pe17 - pe17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Smith Jr., J. Novotny, V. S. Carl, and L. D. Comeau Helicobacter pylori and toll-like receptor agonists induce syndecan-4 expression in an NF-{kappa}B-dependent manner Glycobiology, March 1, 2006; 16(3): 221 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. van den Berg, J. A. E. Spaan, T. M. Rolf, and H. Vink Atherogenic region and diet diminish glycocalyx dimension and increase intima-to-media ratios at murine carotid artery bifurcation Am J Physiol Heart Circ Physiol, February 1, 2006; 290(2): H915 - H920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Haynes III, F. Ruda, J. Oliver, A. N. Hamood, J. A. Griswold, P. W. Park, and K. P. Rumbaugh Syndecan 1 Shedding Contributes to Pseudomonas aeruginosa Sepsis Infect. Immun., December 1, 2005; 73(12): 7914 - 7921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ding, M. Lopez-Burks, J. A. Sanchez-Duran, M. Korc, and A. D. Lander Growth factor-induced shedding of syndecan-1 confers glypican-1 dependence on mitogenic responses of cancer cells J. Cell Biol., November 21, 2005; 171(4): 729 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schmidt, F. Echtermeyer, A. Alozie, K. Brands, and E. Buddecke Plasmin- and Thrombin-accelerated Shedding of Syndecan-4 Ectodomain Generates Cleavage Sites at Lys114-Arg115 and Lys129-Val130 Bonds J. Biol. Chem., October 14, 2005; 280(41): 34441 - 34446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J.R. Singh, J. C. Mason, E. A. Lidington, D. R. Edwards, R. K. Nuttall, R. Khokha, V. Knauper, G. Murphy, and J. Gavrilovic Cytokine stimulated vascular cell adhesion molecule-1 (VCAM-1) ectodomain release is regulated by TIMP-3 Cardiovasc Res, July 1, 2005; 67(1): 39 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Xu, P. W. Park, F. Kheradmand, and D. B. Corry Endogenous Attenuation of Allergic Lung Inflammation by Syndecan-1 J. Immunol., May 1, 2005; 174(9): 5758 - 5765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, V. Vakil, M. Braunstein, E. L. P. Smith, J. Maroney, L. Chen, K. Dai, J. R. Berenson, M. M. Hussain, U. Klueppelberg, et al. Circulating endothelial progenitor cells in multiple myeloma: implications and significance Blood, April 15, 2005; 105(8): 3286 - 3294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Charnaux, S. Brule, T. Chaigneau, L. Saffar, A. Sutton, M. Hamon, C. Prost, N. Lievre, C. Vita, and L. Gattegno RANTES (CCL5) induces a CCR5-dependent accelerated shedding of syndecan-1 (CD138) and syndecan-4 from HeLa cells and forms complexes with the shed ectodomains of these proteoglycans as well as with those of CD44 Glycobiology, February 1, 2005; 15(2): 119 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Houston, M. A. Julien, S. Parthasarathy, and E. L. Chaikof Oxidized linoleic acid regulates expression and shedding of syndecan-4 Am J Physiol Cell Physiol, February 1, 2005; 288(2): C458 - C466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Elenius, M. Gotte, O. Reizes, K. Elenius, and M. Bernfield Inhibition by the Soluble Syndecan-1 Ectodomains Delays Wound Repair in Mice Overexpressing Syndecan-1 J. Biol. Chem., October 1, 2004; 279(40): 41928 - 41935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Deepa, S. Yamada, M. Zako, O. Goldberger, and K. Sugahara Chondroitin Sulfate Chains on Syndecan-1 and Syndecan-4 from Normal Murine Mammary Gland Epithelial Cells Are Structurally and Functionally Distinct and Cooperate with Heparan Sulfate Chains to Bind Growth Factors: A NOVEL FUNCTION TO CONTROL BINDING OF MIDKINE, PLEIOTROPHIN, AND BASIC FIBROBLAST GROWTH FACTOR J. Biol. Chem., September 3, 2004; 279(36): 37368 - 37376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yuan, T.-M. Hong, J. J. W. Chen, W. H. Tsai, and M. T. Lin Syndecan-1 up-regulated by ephrinB2/EphB4 plays dual roles in inflammatory angiogenesis Blood, August 15, 2004; 104(4): 1025 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Mulivor and H. H. Lipowsky Inflammation- and ischemia-induced shedding of venular glycocalyx Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1672 - H1680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Berk, Z. Dong, S. Alousi, M. A. Kosir, Y. Wang, and I. Vlodavsky Murine Ocular Heparanase Expression before and during Infection with Pseudomonas aeruginosa Invest. Ophthalmol. Vis. Sci., April 1, 2004; 45(4): 1182 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Maeda, C. M. Alexander, and A. Friedl Induction of Syndecan-1 Expression in Stromal Fibroblasts Promotes Proliferation of Human Breast Cancer Cells Cancer Res., January 15, 2004; 64(2): 612 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Schulz, W. Annaert, J. Vandekerckhove, P. Zimmermann, B. De Strooper, and G. David Syndecan 3 Intramembrane Proteolysis Is Presenilin/{gamma}-Secretase-dependent and Modulates Cytosolic Signaling J. Biol. Chem., December 5, 2003; 278(49): 48651 - 48657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Endo, T. Takino, H. Miyamori, H. Kinsen, T. Yoshizaki, M. Furukawa, and H. Sato Cleavage of Syndecan-1 by Membrane Type Matrix Metalloproteinase-1 Stimulates Cell Migration J. Biol. Chem., October 17, 2003; 278(42): 40764 - 40770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Marshall, L. S. P. Ramdin, T. Brooks, P. C. DPhil, and J. K. Shute Plasminogen Activator Inhibitor-1 Supports IL-8-Mediated Neutrophil Transendothelial Migration by Inhibition of the Constitutive Shedding of Endothelial IL-8/Heparan Sulfate/Syndecan-1 Complexes J. Immunol., August 15, 2003; 171(4): 2057 - 2065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. H. Chan, D. K. Y. Shum, and M. S. M. Ip Sputum Sol Neutrophil Elastase Activity in Bronchiectasis: Differential Modulation by Syndecan-1 Am. J. Respir. Crit. Care Med., July 15, 2003; 168(2): 192 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, J. Li, C. Partovian, F. W. Sellke, and M. Simons Syndecan-4 modulates basic fibroblast growth factor 2 signaling in vivo Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2078 - H2082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zako, J. Dong, O. Goldberger, M. Bernfield, J. T. Gallagher, and J. A. Deakin Syndecan-1 and -4 Synthesized Simultaneously by Mouse Mammary Gland Epithelial Cells Bear Heparan Sulfate Chains That Are Apparently Structurally Indistinguishable J. Biol. Chem., April 4, 2003; 278(15): 13561 - 13569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. GOTTE Syndecans in inflammation FASEB J, April 1, 2003; 17(6): 575 - 591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Lavelin, N. Meiri, M. Einat, O. Genina, and M. Pines Mechanical strain regulation of the chicken glypican-4 gene expression in the avian eggshell gland Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2002; 283(4): R853 - R861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Joensuu, A. Anttonen, M. Eriksson, R. Makitaro, H. Alfthan, V. Kinnula, and S. Leppa Soluble Syndecan-1 and Serum Basic Fibroblast Growth Factor Are New Prognostic Factors in Lung Cancer Cancer Res., September 15, 2002; 62(18): 5210 - 5217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.C. Weston, I. Haviv, and P.A.W. Rogers Microarray analysis of VEGF-responsive genes in myometrial endothelial cells Mol. Hum. Reprod., September 1, 2002; 8(9): 855 - 863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Johnson, G. J. Brunn, Y. Kodaira, and J. L. Platt Receptor-Mediated Monitoring of Tissue Well-Being Via Detection of Soluble Heparan Sulfate by Toll-Like Receptor 4 J. Immunol., May 15, 2002; 168(10): 5233 - 5239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Denhez, S. A. Wilcox-Adelman, P. C. Baciu, S. Saoncella, S. Lee, B. French, W. Neveu, and P. F. Goetinck Syndesmos, a Syndecan-4 Cytoplasmic Domain Interactor, Binds to the Focal Adhesion Adaptor Proteins Paxillin and Hic-5 J. Biol. Chem., March 29, 2002; 277(14): 12270 - 12274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Li and E. L. Chaikof Mechanical Stress Regulates Syndecan-4 Expression and Redistribution in Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 61 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Huang, R. Chiquet-Ehrismann, J. V. Moyano, A. Garcia-Pardo, and G. Orend Interference of Tenascin-C with Syndecan-4 Binding to Fibronectin Blocks Cell Adhesion and Stimulates Tumor Cell Proliferation Cancer Res., December 1, 2001; 61(23): 8586 - 8594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mechtersheimer, P. Gutwein, N. Agmon-Levin, A. Stoeck, M. Oleszewski, S. Riedle, R. Postina, F. Fahrenholz, M. Fogel, V. Lemmon, et al. Ectodomain shedding of L1 adhesion molecule promotes cell migration by autocrine binding to integrins J. Cell Biol., November 12, 2001; 155(4): 661 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, C. Coomans, and G. David Membrane Heparan Sulfate Proteoglycan-supported FGF2-FGFR1 Signaling. EVIDENCE IN SUPPORT OF THE "COOPERATIVE END STRUCTURES" MODEL J. Biol. Chem., November 2, 2001; 276(45): 41921 - 41929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Landry, V. Rioux, and A. Bensadoun Characterization of Syndecan-4 Expression in 3T3-F442A Mouse Adipocytes: Link between Syndecan-4 Induction and Cell Proliferation Cell Growth Differ., October 1, 2001; 12(10): 497 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-P. Xu, D. Zoukhri, J. D. Zieske, D. A. Dartt, C. Sergheraert, E. Loing, and F.-S. X. Yu A role for MAP kinase in regulating ectodomain shedding of APLP2 in corneal epithelial cells Am J Physiol Cell Physiol, August 1, 2001; 281(2): C603 - C614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. De Vos, G. Couderc, K. Tarte, M. Jourdan, G. Requirand, M.-C. Delteil, J.-F. Rossi, N. Mechti, and B. Klein Identifying intercellular signaling genes expressed in malignant plasma cells by using complementary DNA arrays Blood, August 1, 2001; 98(3): 771 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H Chon and E. L. Chaikof Soluble heparin-binding peptides regulate chemokinesis and cell adhesive forces Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1394 - C1402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Princivalle, S. Hasan, G. Hosseini, and A. I. de Agostini Anticoagulant heparan sulfate proteoglycans expression in the rat ovary peaks in preovulatory granulosa cells Glycobiology, March 1, 2001; 11(3): 183 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Nath, N. Williamson, R Jarvis, and G Murphy Shedding of c-Met is regulated by crosstalk between a G-protein coupled receptor and the EGF receptor and is mediated by a TIMP-3 sensitive metalloproteinase J. Cell Sci., January 3, 2001; 114(6): 1213 - 1220. [Abstract] [PDF]
|
|
|
|
 |
|
 C. E. Bandtlow and D. R. Zimmermann Proteoglycans in the Developing Brain: New Conceptual Insights for Old Proteins Physiol Rev, October 1, 2000; 80(4): 1267 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kishibe, S. Yamada, Y. Okada, J. Sato, A. Ito, K. Miyazaki, and K. Sugahara Structural Requirements of Heparan Sulfate for the Binding to the Tumor-derived Adhesion Factor/Angiomodulin That Induces Cord-like Structures to ECV-304 Human Carcinoma Cells J. Biol. Chem., May 12, 2000; 275(20): 15321 - 15329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gutwein, M. Oleszewski, S. Mechtersheimer, N. Agmon-Levin, K. Krauss, and P. Altevogt Role of Src Kinases in the ADAM-mediated Release of L1 Adhesion Molecule from Human Tumor Cells J. Biol. Chem., May 12, 2000; 275(20): 15490 - 15497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xu, D. Qu, N. L. Esmon, and C. T. Esmon Metalloproteolytic Release of Endothelial Cell Protein C Receptor J. Biol. Chem., February 25, 2000; 275(8): 6038 - 6044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Fitzgerald, Z. Wang, P. W. Park, G. Murphy, and M. Bernfield Shedding of Syndecan-1 and -4 Ectodomains Is Regulated by Multiple Signaling Pathways and Mediated by a TIMP-3-Sensitive Metalloproteinase J. Cell Biol., February 21, 2000; 148(4): 811 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Park, G. B. Pier, M. J. Preston, O. Goldberger, M. L. Fitzgerald, and M. Bernfield Syndecan-1 Shedding Is Enhanced by LasA, a Secreted Virulence Factor of Pseudomonas aeruginosa J. Biol. Chem., February 4, 2000; 275(5): 3057 - 3064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. CHANG, K. MEYER, A. C. RAPRAEGER, and A. FRIEDL Differential ability of heparan sulfate proteoglycans to assemble the fibroblast growth factor receptor complex in situ FASEB J, January 1, 2000; 14(1): 137 - 144. [Abstract] [Full Text]
|
|
|
|
