HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takenaka, S. Articles by Aoki, K. Search for Related Content PubMed PubMed Citation Articles by Takenaka, S. Articles by Aoki, K. Social Bookmarking                      What's this?

Volume 272, Number 23, Issue of June 6, 1997 pp. 14727-14732
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Novel Genes Encoding 2-Aminophenol 1,6-Dioxygenase from Pseudomonas Species AP-3 Growing on 2-Aminophenol and Catalytic Properties of the Purified Enzyme

(Received for publication, February 26, 1997, and in revised form, April 4, 1997)

Shinji Takenaka , Shuichiro Murakami , Ryu Shinke , Kazuhisa Hatakeyama ^§ , Hideaki Yukawa ^§ and Kenji Aoki

From the Division of Science of Biological Resources, Graduate School of Science and Technology and the  Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Rokko, Kobe 657 and ^§ Tsukuba Research Center, Mitsubishi Chemical, Ibaraki 300-03, Japan

2-Aminophenol 1,6-dioxygenase was purified from the cell extracts of Pseudomonas sp. AP-3 grown on 2-aminophenol. The product from 2-aminophenol by catalysis of the purified enzyme was identified as 2-aminomuconic 6-semialdehyde by gas chromatographic and mass spectrometric analyses. The molecular mass of the native enzyme was 140 kDa based on gel filtration. It was dissociated into molecular mass subunits of 32 (-subunit) and 40 kDa (-subunit) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating that the dioxygenase was a heterotetramer of ₂₂. The genes coding for the - and -subunits of the enzyme were cloned and sequenced. Open reading frames of the genes (amnA and amnB) were 816 and 918 base pairs in length, respectively. The amino acid sequences predicted from the open reading frames of amnA and amnB corresponded to the NH₂-terminal amino acid sequences of the -subunit (AmnA) and -subunit (AmnB), respectively. The deduced amino acid sequences of AmnB showed identities to some extent with HpaD (25.4%) and HpcB (24.4%) that are homoprotocatechuate 2,3-dioxygenases from Escherichia coli W and C, respectively, belonging to class III in the extradiol dioxygenases. On the other hand, AmnA had identity (23.3%) with only AmnB among the enzymes examined.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. J. H. Moonen, S. A. Synowsky, W. A. M. van den Berg, A. H. Westphal, A. J. R. Heck, R. H. H. van den Heuvel, M. W. Fraaije, and W. J. H. van Berkel Hydroquinone Dioxygenase from Pseudomonas fluorescens ACB: a Novel Member of the Family of Nonheme-Iron(II)-Dependent Dioxygenases J. Bacteriol., August 1, 2008; 190(15): 5199 - 5209. [Abstract] [Full Text] [PDF]

				M. Gaillard, T. Vallaeys, F. J. Vorholter, M. Minoia, C. Werlen, V. Sentchilo, A. Puhler, and J. R. van der Meer The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties. J. Bacteriol., March 1, 2006; 188(5): 1999 - 2013. [Abstract] [Full Text] [PDF]

				J. Nogales, A. Canales, J. Jimenez-Barbero, J. L. Garcia, and E. Diaz Molecular Characterization of the Gallate Dioxygenase from Pseudomonas putida KT2440: THE PROTOTYPE OF A NEW SUBGROUP OF EXTRADIOL DIOXYGENASES J. Biol. Chem., October 21, 2005; 280(42): 35382 - 35390. [Abstract] [Full Text] [PDF]

				J.-P. Hintner, T. Reemtsma, and A. Stolz Biochemical and Molecular Characterization of a Ring Fission Dioxygenase with the Ability to Oxidize (Substituted) Salicylate(s) from Pseudaminobacter salicylatoxidans J. Biol. Chem., September 3, 2004; 279(36): 37250 - 37260. [Abstract] [Full Text] [PDF]

				S. Takenaka, S. Okugawa, M. Kadowaki, S. Murakami, and K. Aoki The Metabolic Pathway of 4-Aminophenol in Burkholderia sp. Strain AK-5 Differs from That of Aniline and Aniline with C-4 Substituents Appl. Envir. Microbiol., September 1, 2003; 69(9): 5410 - 5413. [Abstract] [Full Text] [PDF]

				J.-P. Hintner, C. Lechner, U. Riegert, A. E. Kuhm, T. Storm, T. Reemtsma, and A. Stolz Direct Ring Fission of Salicylate by a Salicylate 1,2-Dioxygenase Activity from Pseudaminobacter salicylatoxidans J. Bacteriol., December 1, 2001; 183(23): 6936 - 6942. [Abstract] [Full Text] [PDF]

				H.-S. Park and H.-S. Kim Genetic and Structural Organization of the Aminophenol Catabolic Operon and Its Implication for Evolutionary Process J. Bacteriol., September 1, 2001; 183(17): 5074 - 5081. [Abstract] [Full Text] [PDF]

				J. K. Davis, G. C. Paoli, Z. He, L. J. Nadeau, C. C. Somerville, and J. C. Spain Sequence Analysis and Initial Characterization of Two Isozymes of Hydroxylaminobenzene Mutase from Pseudomonas pseudoalcaligenes JS45 Appl. Envir. Microbiol., July 1, 2000; 66(7): 2965 - 2971. [Abstract] [Full Text]

				Z. He and J. C. Spain Reactions Involved in the Lower Pathway for Degradation of 4-Nitrotoluene by Mycobacterium Strain HL 4-NT-1 Appl. Envir. Microbiol., July 1, 2000; 66(7): 3010 - 3015. [Abstract] [Full Text]

				E. Katsivela, V. Wray, D. H. Pieper, and R.-M. Wittich Initial Reactions in the Biodegradation of 1-Chloro-4-Nitrobenzene by a Newly Isolated Bacterium, Strain LW1 Appl. Envir. Microbiol., April 1, 1999; 65(4): 1405 - 1412. [Abstract] [Full Text]

				Z. He, J. K. Davis, and J. C. Spain Purification, Characterization, and Sequence Analysis of 2-Aminomuconic 6-Semialdehyde Dehydrogenase from Pseudomonas pseudoalcaligenes JS45 J. Bacteriol., September 1, 1998; 180(17): 4591 - 4595. [Abstract] [Full Text]