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Volume 272, Number 24, Issue of June 13, 1997 pp. 15426-15433
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

A Role for Protein Kinase CI in the Regulation of Ca²⁺ Entry in Jurkat T Cells

(Received for publication, December 30, 1996, and in revised form, March 24, 1997)

Doris M. Haverstick , Michael Dicus , Moira S. Resnick ^¶ , Julianne J. Sando ^¶ and Lloyd S. Gray

From the Departments of  Pathology and ^¶ Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia 22908

T cell activation leading to cytokine production and cellular proliferation involves a regulated increase and subsequent decrease in the intracellular concentration of Ca²⁺ ([Ca²⁺]_i). While much is understood about agonist-induced increases in [Ca²⁺]_i, less is known about down-regulation of this pathway. Understanding the mechanism of this down-regulation is critical to the prevention of cell death that can be the consequence of a sustained elevation in [Ca²⁺]_i. Protein kinase C (PKC), activated by the diacylglycerol produced as a consequence of T cell receptor engagement, has long been presumed to be involved in this down-regulation, although the precise mechanism is not wholly clear. In this report we demonstrate that activation of PKC by phorbol esters slightly decreases the rate of Ca²⁺ efflux from the cytosol of Jurkat T cells following stimulation through the T cell receptor or stimulation in a receptor-independent manner by thapsigargin. On the other hand, phorbol ester treatment dramatically reduces the rate of Ca²⁺ influx following stimulation. Phorbol ester treatment is without an effect on Ca²⁺ influx in a different T cell line, HSB. Down-regulation of PKCI expression by 18-h phorbol ester treatment is associated with a loss of the response to acute phorbol ester treatment in Jurkat cells, suggesting that PKCI may be the isozyme responsible for the effects on Ca²⁺ influx. Electroporation of an anti-PKCI antibody, but not antibodies against PKC or PKC, led to an increase in the rate of Ca²⁺ influx following stimulation. Taken together, these data suggest that PKCI may be a component of the down-regulation of increases in [Ca²⁺]_i associated with Jurkat T cell activation.

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