HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Uesono, Y. Articles by Kikuchi, Y. Search for Related Content PubMed PubMed Citation Articles by Uesono, Y. Articles by Kikuchi, Y. Social Bookmarking                      What's this?

Volume 272, Number 26, Issue of June 27, 1997 pp. 16103-16109
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Ssd1p of Saccharomyces cerevisiae Associates with RNA

(Received for publication, April 25, 1997)

From the Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan

The SSD1 gene has been isolated as a single copy suppressor of many mutants, such as sit4, slk1/bck1, pde2, and rpc31, in the yeast Saccharomyces cerevisiae. Ssd1p has domains showing weak but significant homology with RNase II-related proteins, Cyt4p, Dss1p, VacB, and RNase II, which are involved in the modification of RNA. We found that Ssd1p had the ability to bind RNA, preferably poly(rA), as well as single-stranded DNA. Interestingly, the most conserved domain among the RNase II-related proteins was not necessary for interaction with RNA. Indirect immunofluorescence staining with anti-Ssd1p antibody revealed that Ssd1p was detected mainly in the cytoplasm. Furthermore, sucrose gradient sedimentation analysis demonstrated that Ssd1p was not cofractionated with polyribosomes, suggesting that Ssd1p is not particularly bound to a translationally active subpopulation of mRNA in the cytoplasm.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Schneider, E. Leung, J. Brown, and D. Tollervey The N-terminal PIN domain of the exosome subunit Rrp44 harbors endonuclease activity and tethers Rrp44 to the yeast core exosome Nucleic Acids Res., March 1, 2009; 37(4): 1127 - 1140. [Abstract] [Full Text] [PDF]

				S. S. Mir, D. Fiedler, and A. G. Cashikar Ssd1 Is Required for Thermotolerance and Hsp104-Mediated Protein Disaggregation in Saccharomyces cerevisiae Mol. Cell. Biol., January 1, 2009; 29(1): 187 - 200. [Abstract] [Full Text] [PDF]

				K. D. Gank, M. R. Yeaman, S. Kojima, N. Y. Yount, H. Park, J. E. Edwards Jr., S. G. Filler, and Y. Fu SSD1 Is Integral to Host Defense Peptide Resistance in Candida albicans Eukaryot. Cell, August 1, 2008; 7(8): 1318 - 1327. [Abstract] [Full Text] [PDF]

				S. Seiler, N. Vogt, C. Ziv, R. Gorovits, and O. Yarden The STE20/Germinal Center Kinase POD6 Interacts with the NDR Kinase COT1 and Is Involved in Polar Tip Extension in Neurospora crassa Mol. Biol. Cell, September 1, 2006; 17(9): 4080 - 4092. [Abstract] [Full Text] [PDF]

				Y. Jiang Regulation of the Cell Cycle by Protein Phosphatase 2A in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 440 - 449. [Abstract] [Full Text] [PDF]

				N. Dephoure, R. W. Howson, J. D. Blethrow, K. M. Shokat, and E. K. O'Shea Combining chemical genetics and proteomics to identify protein kinase substrates PNAS, December 13, 2005; 102(50): 17940 - 17945. [Abstract] [Full Text] [PDF]

				C. Kurischko, G. Weiss, M. Ottey, and F. C. Luca A Role for the Saccharomyces cerevisiae Regulation of Ace2 and Polarized Morphogenesis Signaling Network in Cell Integrity Genetics, October 1, 2005; 171(2): 443 - 455. [Abstract] [Full Text] [PDF]

				A. Reinke, S. Anderson, J. M. McCaffery, J. Yates III, S. Aronova, S. Chu, S. Fairclough, C. Iverson, K. P. Wedaman, and T. Powers TOR Complex 1 Includes a Novel Component, Tco89p (YPL180w), and Cooperates with Ssd1p to Maintain Cellular Integrity in Saccharomyces cerevisiae J. Biol. Chem., April 9, 2004; 279(15): 14752 - 14762. [Abstract] [Full Text] [PDF]

				M. Kaeberlein, A. A. Andalis, G. B. Liszt, G. R. Fink, and L. Guarente Saccharomyces cerevisiae SSD1-V Confers Longevity by a Sir2p-Independent Mechanism Genetics, April 1, 2004; 166(4): 1661 - 1672. [Abstract] [Full Text] [PDF]

				K. Ohkuni, A. Okuda, and A. Kikuchi Yeast Nap1-Binding Protein Nbp2p Is Required for Mitotic Growth at High Temperatures and for Cell Wall Integrity Genetics, October 1, 2003; 165(2): 517 - 529. [Abstract] [Full Text] [PDF]

				T. Singer, S. Haefner, M. Hoffmann, M. Fischer, J. Ilyina, and W. Hilt Sit4 Phosphatase Is Functionally Linked to the Ubiquitin-Proteasome System Genetics, August 1, 2003; 164(4): 1305 - 1321. [Abstract] [Full Text] [PDF]

				K. Vincent, Q. Wang, S. Jay, K. Hobbs, and B. C. Rymond Genetic Interactions With CLF1 Identify Additional Pre-mRNA Splicing Factors and a Link Between Activators of Yeast Vesicular Transport and Splicing Genetics, July 1, 2003; 164(3): 895 - 907. [Abstract] [Full Text] [PDF]

				R. T. Wheeler, M. Kupiec, P. Magnelli, C. Abeijon, and G. R. Fink A Saccharomyces cerevisiae mutant with increased virulence PNAS, March 4, 2003; 100(5): 2766 - 2770. [Abstract] [Full Text] [PDF]

				P. Jorgensen, B. Nelson, M. D. Robinson, Y. Chen, B. Andrews, M. Tyers, and C. Boone High-Resolution Genetic Mapping With Ordered Arrays of Saccharomyces cerevisiae Deletion Mutants Genetics, November 1, 2002; 162(3): 1091 - 1099. [Abstract] [Full Text] [PDF]

				H. B. McDonald, A. H. Helfant, E. M. Mahony, S. K. Khosla, and L. Goetsch Mutational Analysis Reveals a Role for the C Terminus of the Proteasome Subunit Rpt4p in Spindle Pole Body Duplication in Saccharomyces cerevisiae Genetics, October 1, 2002; 162(2): 705 - 720. [Abstract] [Full Text] [PDF]

				Y. Uesono and A. Toh-e Transient Inhibition of Translation Initiation by Osmotic Stress J. Biol. Chem., April 12, 2002; 277(16): 13848 - 13855. [Abstract] [Full Text] [PDF]

				G. M. Euskirchen Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae Eukaryot. Cell, April 1, 2002; 1(2): 229 - 240. [Abstract] [Full Text] [PDF]

				M. Kaeberlein and L. Guarente Saccharomyces cerevisiae MPT5 and SSD1 Function in Parallel Pathways to Promote Cell Wall Integrity Genetics, January 1, 2002; 160(1): 83 - 95. [Abstract] [Full Text] [PDF]

				M. Bucheli, L. Lommel, and K. Sweder The Defect in Transcription-Coupled Repair Displayed by a Saccharomyces cerevisiae rad26 Mutant Is Dependent on Carbon Source and Is Not Associated With a Lack of Transcription Genetics, July 1, 2001; 158(3): 989 - 997. [Abstract] [Full Text] [PDF]

				Y. Zuo and M. P. Deutscher Exoribonuclease superfamilies: structural analysis and phylogenetic distribution Nucleic Acids Res., March 1, 2001; 29(5): 1017 - 1026. [Abstract] [Full Text] [PDF]

				P. Fortes, J. Kufel, M. Fornerod, M. Polycarpou-Schwarz, D. Lafontaine, D. Tollervey, and I. W. Mattaj Genetic and Physical Interactions Involving the Yeast Nuclear Cap-Binding Complex Mol. Cell. Biol., October 1, 1999; 19(10): 6543 - 6553. [Abstract] [Full Text] [PDF]

				J. Drgonova, T. Drgon, D.-H. Roh, and E. Cabib The GTP-binding Protein Rho1p Is Required for Cell Cycle Progression and Polarization of the Yeast Cell J. Cell Biol., July 26, 1999; 146(2): 373 - 388. [Abstract] [Full Text] [PDF]

				M. D. Mendenhall and A. E. Hodge Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1191 - 1243. [Abstract] [Full Text] [PDF]

				L.-L. Du and P. Novick Pag1p, a Novel Protein Associated with Protein Kinase Cbk1p, Is Required for Cell Morphogenesis and Proliferation in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2002; 13(2): 503 - 514. [Abstract] [Full Text] [PDF]