HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Marjamaki, A. Articles by Lanier, S. M. Search for Related Content PubMed PubMed Citation Articles by Marjamaki, A. Articles by Lanier, S. M. Social Bookmarking                      What's this?

Volume 272, Number 26, Issue of June 27, 1997 pp. 16466-16473
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Factors Determining the Specificity of Signal Transduction by Guanine Nucleotide-binding Protein-coupled Receptors
INTEGRATION OF STIMULATORY AND INHIBITORY INPUT TO THE EFFECTOR ADENYLYL CYCLASE

(Received for publication, December 31, 1996, and in revised form, March 5, 1997)

From the Department of Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29425 and ^§ Laboratorie de Physiologie de la Reproduction, CNRS URA 1449, Universite Pierre et Marie Curie, 75252 Paris, France

To define the integration of multiple signals by different types of adenylyl cyclase (AC) within the cell, we altered the population of enzymes expressed in the cell and determined the subsequent processing of stimulatory and inhibitory input. DDT₁-MF2 cells expressed AC VI-IX and were stably transfected with AC II, III, or IV. Enzyme expression was confirmed by RNA blot analysis and functional assays. Basal enzyme activity was only increased in AC II transfectants (6-fold). Maximum stimulation of enzyme activity was increased in each of the AC transfectants to varying extents. _2A/D-AR activation elicited enzyme type-specific responses. ₂-AR activation inhibited the effect of isoproterenol in control transfectants, and this action was magnified in AC III transfectants. In AC II and AC IV transfectants, ₂-AR activation initiated both positive (G) and negative signals (G_i) to the G_s-stimulated enzyme, and both types of signals were blocked by cell pretreatment with pertussis toxin. The negative input to AC II from the ₂-AR was blocked by protein kinase C activation in AC II transfectants, but it was the positive input to AC IV that was compromised by protein kinase C activation. These data indicate that the integration of multiple signals by adenylyl cyclases is a dynamic process depending upon the enzyme type and phosphorylation status.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. Yamada, T. Inagaki, R. Funahashi, Y. Yoshimura, and Y. Komatsu High-Frequency Stimulation Together with Adrenoceptor Activation Facilitates the Maintenance of Long-Term Potentiation at Visual Cortical Inhibitory Synapses Cereb Cortex, September 1, 2006; 16(9): 1239 - 1248. [Abstract] [Full Text] [PDF]

				T. Holmqvist, L. Johansson, M. Ostman, S. Ammoun, K. E. O. Akerman, and J. P. Kukkonen OX1 Orexin Receptors Couple to Adenylyl Cyclase Regulation via Multiple Mechanisms J. Biol. Chem., February 25, 2005; 280(8): 6570 - 6579. [Abstract] [Full Text] [PDF]

				M. Dupuis, A. Levy, and S. Mhaouty-Kodja Functional Coupling of Rat Myometrial {alpha}1-Adrenergic Receptors to Gh{alpha}/Tissue Transglutaminase 2 during Pregnancy J. Biol. Chem., April 30, 2004; 279(18): 19257 - 19263. [Abstract] [Full Text] [PDF]

				T. Abaffy, K. R. Trubey, and N. Chaudhari Adenylyl cyclase expression and modulation of cAMP in rat taste cells Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1420 - C1428. [Abstract] [Full Text] [PDF]

				P. Bianchi, M.-H. Seguelas, A. Parini, and C. Cambon Activation of Pro-Apoptotic Cascade by Dopamine in Renal Epithelial Cells is Fully Dependent on Hydrogen Peroxide Generation by Monoamine Oxidases J. Am. Soc. Nephrol., April 1, 2003; 14(4): 855 - 862. [Abstract] [Full Text] [PDF]

				C. Ribas, A. Takesono, M. Sato, J. D. Hildebrandt, and S. M. Lanier Pertussis Toxin-insensitive Activation of the Heterotrimeric G-proteins Gi/Go by the NG108-15 G-protein Activator J. Biol. Chem., December 20, 2002; 277(52): 50223 - 50225. [Abstract] [Full Text] [PDF]

				J. G. Webb, P. W. Yates, Q. Yang, Y. V. Mukhin, and S. M. Lanier Adenylyl cyclase isoforms and signal integration in models of vascular smooth muscle cells Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1545 - H1552. [Abstract] [Full Text] [PDF]

				M. Cote, G. Guillon, M. D. Payet, and N. Gallo-Payet Expression and Regulation of Adenylyl Cyclase Isoforms in the Human Adrenal Gland J. Clin. Endocrinol. Metab., September 1, 2001; 86(9): 4495 - 4503. [Abstract] [Full Text] [PDF]

				K. B. Jourdan, N. A. Mason, L. Long, P. G. Philips, M. R. Wilkins, and N. W. Morrell Characterization of adenylyl cyclase isoforms in rat peripheral pulmonary arteries Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1359 - L1369. [Abstract] [Full Text] [PDF]

				I. Limon-Boulez, R. Bouet-Alard, T. W. Gettys, S. M. Lanier, J.-P. Maltier, and C. Legrand Partial Agonist Clonidine Mediates {alpha}2-AR Subtypes Specific Regulation of cAMP Accumulation in Adenylyl Cyclase II Transfected DDT1-MF2 Cells Mol. Pharmacol., February 1, 2001; 59(2): 331 - 338. [Abstract] [Full Text]

				N. Defer, M. Best-Belpomme, and J. Hanoune Tissue specificity and physiological relevance of various isoforms of adenylyl cyclase Am J Physiol Renal Physiol, September 1, 2000; 279(3): F400 - F416. [Abstract] [Full Text] [PDF]

				Y. F. Liu, M. H. Ghahremani, M. M. Rasenick, K. H. Jakobs, and P. R. Albert Stimulation of cAMP Synthesis by Gi-coupled Receptors upon Ablation of Distinct Galpha i Protein Expression. Gi SUBTYPE SPECIFICITY OF THE 5-HT1A RECEPTOR J. Biol. Chem., June 4, 1999; 274(23): 16444 - 16450. [Abstract] [Full Text] [PDF]

				P.-Y. Law and H. H. Loh Regulation of Opioid Receptor Activities J. Pharmacol. Exp. Ther., May 1, 1999; 289(2): 607 - 624. [Abstract] [Full Text]

				J. J. Evans Modulation of Gonadotropin Levels by Peptides Acting at the Anterior Pituitary Gland Endocr. Rev., February 1, 1999; 20(1): 46 - 67. [Abstract] [Full Text]

				S. Mhaouty-Kodja, R. Bouet-Alard, I. Limon-Boulez, J. P. Maltier, and C. Legrand Molecular Diversity of Adenylyl Cyclases in Human and Rat Myometrium. CORRELATION WITH GLOBAL ADENYLYL CYCLASE ACTIVITY DURING MID- AND TERM PREGNANCY J. Biol. Chem., December 5, 1997; 272(49): 31100 - 31106. [Abstract] [Full Text] [PDF]