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(Received for publication, November 26, 1996, and in revised form, March 22, 1997)
From the The properties of duplex CTG·CAG and CGG·CCG,
which are involved in the etiology of several hereditary
neurodegenerative diseases, were investigated by a variety of methods,
including circularization kinetics, apparent helical repeat
determination, and polyacrylamide gel electrophoresis. The bending
moduli were 1.13 × 10
Volume 272, Number 27,
Issue of July 4, 1997
pp. 16783-16792
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
,
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,
,
,
,
,
and
Center for Genome Research, Institute of
Biosciences and Technology and the Department of Biochemistry and
Biophysics, Texas A & M University, Texas Medical Center, 2121 Holcombe
Blvd., Houston, Texas 77030, the ¶ Department of Biochemistry
and Molecular Genetics, University of Alabama at Birmingham, School of
Medicine, Birmingham, Alabama 35294-0005, and the 
Department of
Biochemistry, Tufts University, School of Medicine, 136 Harrison
Ave., Boston, Massachusetts 02111
19 erg·cm for CTG and
1.27 × 1019 erg·cm for CGG, ~40% less than for
random B-DNA. Also, the persistence lengths of the triplet repeat
sequences were ~60% the value for random B-DNA. However, the
torsional moduli and the helical repeats were 2.3 × 1019 erg·cm and 10.4 base pairs (bp)/turn for CTG and
2.4 × 1019 erg·cm and 10.3 bp/turn for CGG,
respectively, all within the range for random B-DNA. Determination of
the apparent helical repeat by the band shift assay indicated that the
writhe of the repeats was different from that of random B-DNA. In
addition, molecules of 224-245 bp in length (64-71 triplet repeats)
were able to form topological isomers upon cyclization. The low bending moduli are consistent with predictions from crystallographic variations in slide, roll, and tilt. No unpaired bases or non-B-DNA structures could be detected by chemical and enzymatic probe analyses,
two-dimensional agarose gel electrophoresis, and immunological studies.
Hence, CTG and CGG are more flexible and highly writhed than random
B-DNA and thus would be expected to act as sinks for the accumulation of superhelical density.
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