HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Boylan, M. O. Articles by Wolfe, M. M. Search for Related Content PubMed PubMed Citation Articles by Boylan, M. O. Articles by Wolfe, M. M. Social Bookmarking                      What's this?

Volume 272, Number 28, Issue of July 11, 1997 pp. 17438-17443
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Cell-specific Expression of the Glucose-dependent Insulinotropic Polypeptide Gene in a Mouse Neuroendocrine Tumor Cell Line

(Received for publication, February 19, 1997)

From the Section of Gastroenterology, Boston University School of Medicine and Boston Medical Center, Boston, Massachusetts 02118

Glucose-dependent insulinotropic polypeptide (GIP) is a 42-amino acid gastrointestinal regulatory peptide that, in the presence of glucose, stimulates insulin secretion. GIP is expressed in K cells of the small intestine and in cells of the submandibular salivary gland. Using a rat GIP cDNA as a specific probe, we screened a number of established cell lines for the expression of GIP mRNA. STC-1 cells, a cell line derived from a mouse neuroendocrine tumor, were found to express high levels of GIP mRNA. GIP-specific transcripts were not detected in other cell lines tested, which included cells of intestinal, salivary, and endocrine origin. Analysis of GIP-luciferase fusions identified two promoters, a distal and a proximal promoter, upstream of the translation initiation codon for GIP. The distal promoter, located upstream of position +1, corresponds to the principal promoter of the GIP gene and can promote cell-specific transcription. Sequential deletion and site-directed mutational analysis of the distal promoter demonstrated that the sequence between 193 and 182 determines cell-specific expression of GIP. Contained in this region is a consensus GATA motif, suggesting that a member of the GATA family of DNA-binding proteins is involved in the cell-specific regulation of the GIP gene.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				W. Kim and J. M. Egan The Role of Incretins in Glucose Homeostasis and Diabetes Treatment Pharmacol. Rev., December 1, 2008; 60(4): 470 - 512. [Abstract] [Full Text] [PDF]

				Y. Fujita, J. W. Y. Chui, D. S. King, T. Zhang, J. Seufert, S. Pownall, A. T. Cheung, and T. J. Kieffer Pax6 and Pdx1 are required for production of glucose-dependent insulinotropic polypeptide in proglucagon-expressing L cells Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E648 - E657. [Abstract] [Full Text] [PDF]

				L. I. Jepeal, Y. Fujitani, M. O. Boylan, C. N. Wilson, C. V. Wright, and M. M. Wolfe Cell-Specific Expression of Glucose-Dependent-Insulinotropic Polypeptide Is Regulated by the Transcription Factor PDX-1 Endocrinology, January 1, 2005; 146(1): 383 - 391. [Abstract] [Full Text] [PDF]

				C. H. Chang, W. Y. Chey, B. Erway, D. H. Coy, and T.-M. Chang Modulation of secretin release by neuropeptides in secretin-producing cells Am J Physiol Gastrointest Liver Physiol, August 1, 1998; 275(2): G192 - G202. [Abstract] [Full Text] [PDF]

				J. Lovshin, J. Estall, B. Yusta, T. J. Brown, and D. J. Drucker Glucagon-like Peptide (GLP)-2 Action in the Murine Central Nervous System Is Enhanced by Elimination of GLP-1 Receptor Signaling J. Biol. Chem., June 8, 2001; 276(24): 21489 - 21499. [Abstract] [Full Text] [PDF]