Volume 272, Number 29, Issue of July 18, 1997 pp. 18418-18424
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Syndecan-1 Expression Is Down-regulated during Myoblast Terminal Differentiation
MODULATION BY GROWTH FACTORS AND RETINOIC ACID

(Received for publication, January 31, 1997, and in revised form, May 15, 1997)

Juan Larraín , Gunay Cizmeci-Smith ^¶ , Victor Troncoso , Richard C. Stahl ^¶ , David J. Carey ^¶ and Enrique Brandan

From the  Department of Cell and Molecular Biology, Faculty of Biological Sciences, Catholic University of Chile, Casilla 114-D, Santiago, Chile, and the ^¶ Sigfried and Janet Weis Center for Research, Danville, Pennsylvania 17822

Syndecan-1 is an integral membrane proteoglycan involved in the interaction of cells with extracellular matrix proteins and growth factors. It is transiently expressed in several condensing mesenchymal tissues after epithelial induction. In this study we evaluated the expression of syndecan-1 during skeletal muscle differentiation. The expression of syndecan-1 as determined by Northern blot analyses and immunofluorescence microscopy is down-regulated during differentiation. The transcriptional activity of a syndecan-1 promoter construct is also down-regulated in differentiating muscle cells. The decrease in syndecan-1 gene expression is not dependent on the presence of E-boxes, binding sites for the MyoD family of transcription factors in the promoter region, or myogenin expression. Deletion of the region containing the E-boxes or treatment of differentiating cells with sodium butyrate, an inhibitor of myogenin expression, had no effect on syndecan-1 expression. Basic fibroblast growth factor and transforming growth factor type , which are inhibitors of myogenesis, had little effect on syndecan-1 expression. When added together, however, they induced syndecan-1 expression. Retinoic acid, an inducer of myogenesis, inhibited syndecan-1 expression and abolished the effect of the growth factors. These results indicate that syndecan-1 expression is down-regulated during myogenesis and that growth factors and retinoic acid modulate syndecan-1 expression by a mechanism that is independent of myogenin.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. G. Velleman, C. S. Coy, and D. C. McFarland Effect of Syndecan-1, Syndecan-4, and Glypican-1 on Turkey Muscle Satellite Cell Proliferation, Differentiation, and Responsiveness to Fibroblast Growth Factor 2 Poult. Sci., July 1, 2007; 86(7): 1406 - 1413. [Abstract] [Full Text] [PDF]

				S. G. Velleman Muscle Development in the Embryo and Hatchling Poult. Sci., May 1, 2007; 86(5): 1050 - 1054. [Abstract] [Full Text] [PDF]

				E. Brandan, C. Retamal, C. Cabello-Verrugio, and M.-P. Marzolo The Low Density Lipoprotein Receptor-related Protein Functions as an Endocytic Receptor for Decorin J. Biol. Chem., October 20, 2006; 281(42): 31562 - 31571. [Abstract] [Full Text] [PDF]

				D.D.W. Cornelison, S. A. Wilcox-Adelman, P. F. Goetinck, H. Rauvala, A. C. Rapraeger, and B. B. Olwin Essential and separable roles for Syndecan-3 and Syndecan-4 in skeletal muscle development and regeneration Genes & Dev., September 15, 2004; 18(18): 2231 - 2236. [Abstract] [Full Text] [PDF]

				J. C. Casar, C. Cabello-Verrugio, H. Olguin, R. Aldunate, N. C. Inestrosa, and E. Brandan Heparan sulfate proteoglycans are increased during skeletal muscle regeneration: requirement of syndecan-3 for successful fiber formation J. Cell Sci., January 1, 2004; 117(1): 73 - 84. [Abstract] [Full Text] [PDF]

				I. Barbosa, S. Garcia, V. Barbier-Chassefiere, J.-P. Caruelle, I. Martelly, and D. Papy-Garcia Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies Glycobiology, September 1, 2003; 13(9): 647 - 653. [Abstract] [Full Text] [PDF]

				F. Lopez-Casillas, C. Riquelme, Y. Perez-Kato, M. V. Ponce-Castaneda, N. Osses, J. Esparza-Lopez, G. Gonzalez-Nunez, C. Cabello-Verrugio, V. Mendoza, V. Troncoso, et al. Betaglycan Expression Is Transcriptionally Up-regulated during Skeletal Muscle Differentiation. CLONING OF MURINE BETAGLYCAN GENE PROMOTER AND ITS MODULATION BY MyoD, RETINOIC ACID, AND TRANSFORMING GROWTH FACTOR-beta J. Biol. Chem., January 3, 2003; 278(1): 382 - 390. [Abstract] [Full Text] [PDF]

				J. P. Henriquez, J. C. Casar, L. Fuentealba, D. J. Carey, and E. Brandan Extracellular matrix histone H1 binds to perlecan, is present in regenerating skeletal muscle and stimulates myoblast proliferation J. Cell Sci., May 15, 2002; 115(10): 2041 - 2051. [Abstract] [Full Text] [PDF]

				G. J. Jenniskens, A. Oosterhof, R. Brandwijk, J. H. Veerkamp, and T. H. van Kuppevelt Heparan Sulfate Heterogeneity in Skeletal Muscle Basal Lamina: Demonstration by Phage Display-Derived Antibodies J. Neurosci., June 1, 2000; 20(11): 4099 - 4111. [Abstract] [Full Text] [PDF]

				L. Fuentealba, D. J. Carey, and E. Brandan Antisense Inhibition of Syndecan-3 Expression during Skeletal Muscle Differentiation Accelerates Myogenesis through a Basic Fibroblast Growth Factor-dependent Mechanism J. Biol. Chem., December 31, 1999; 274(53): 37876 - 37884. [Abstract] [Full Text] [PDF]

				J. Larrain, D. J. Carey, and E. Brandan Syndecan-1 Expression Inhibits Myoblast Differentiation through a Basic Fibroblast Growth Factor-dependent Mechanism J. Biol. Chem., November 27, 1998; 273(48): 32288 - 32296. [Abstract] [Full Text] [PDF]

				C. Riquelme, J. Larrain, E. Schonherr, J. P. Henriquez, H. Kresse, and E. Brandan Antisense Inhibition of Decorin Expression in Myoblasts Decreases Cell Responsiveness to Transforming Growth Factor beta and Accelerates Skeletal Muscle Differentiation J. Biol. Chem., January 26, 2001; 276(5): 3589 - 3596. [Abstract] [Full Text] [PDF]

				N. Osses and E. Brandan ECM is required for skeletal muscle differentiation independently of muscle regulatory factor expression Am J Physiol Cell Physiol, February 1, 2002; 282(2): C383 - C394. [Abstract] [Full Text] [PDF]