Volume 272, Number 3, Issue of January 17, 1997 pp. 1421-1424
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

---

COMMUNICATION:
Spodoptera frugiperda Caspase-1, a Novel Insect Death Protease That Cleaves the Nuclear Immunophilin FKBP46, Is the Target of the Baculovirus Antiapoptotic Protein p35

(Received for publication, October 1, 1996, and in revised form, November 22, 1996)

From the Center for Apoptosis Research, the Department of Biochemistry and Molecular Pharmacology, and the Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, Pennsylvania 19107

Employing the degenerate primer-dependent polymerase chain reaction approach used recently to clone human Mch2, we have identified and cloned the insect Spodoptera frugiperda target of the baculovirus antiapoptotic protein p35. This protein named Sf caspase-1 belongs to the family of caspases and is highly related to human Mch3 and CPP32 in sequence and specific activity. The proenzyme of Sf caspase-1 is 299 amino acids in length and can undergo autocatalytic processing in Escherichia coli to an active enzyme heterocomplex. Autoprocessing occurs at Asp-28, Asp-184, and Asp-195 to generate the large p19/p18 and small p12 subunits. Sf caspase-1 is able to induce apoptosis in Sf9 cells and is capable of cleaving p35 to similar sized fragments as observed with extracts from p35 null mutant baculovirus-infected Sf9 cells. Sf caspase-1 activity is potently inhibited by p35, suggesting that it is an important target of this antiapoptotic protein. Finally, the Sf9 nuclear immunophilin FKBP46 was identified as a death-associated substrate for Sf caspase-1.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. Lapointe, R. Wilson, L. Vilaplana, D. R. O'Reilly, P. Falabella, V. Douris, M. Bernier-Cardou, F. Pennacchio, K. Iatrou, C. Malva, et al. Expression of a Toxoneuron nigriceps polydnavirus-encoded protein causes apoptosis-like programmed cell death in lepidopteran insect cells J. Gen. Virol., April 1, 2005; 86(4): 963 - 971. [Abstract] [Full Text] [PDF]

				R. Nathaniel, A. L. MacNeill, Y.-X. Wang, P. C. Turner, and R. W. Moyer Cowpox virus CrmA, Myxoma virus SERP2 and baculovirus P35 are not functionally interchangeable caspase inhibitors in poxvirus infections J. Gen. Virol., May 1, 2004; 85(5): 1267 - 1278. [Abstract] [Full Text] [PDF]

				C. M. Forsyth, D. Lemongello, D. J. LaCount, P. D. Friesen, and A. J. Fisher Crystal Structure of an Invertebrate Caspase J. Biol. Chem., February 20, 2004; 279(8): 7001 - 7008. [Abstract] [Full Text] [PDF]

				H. Ishikawa, M. Ikeda, K. Yanagimoto, C. A. F. Alves, Y. Katou, B. A. Lavina-Caoili, and M. Kobayashi Induction of apoptosis in an insect cell line, IPLB-Ld652Y, infected with nucleopolyhedroviruses J. Gen. Virol., March 1, 2003; 84(3): 705 - 714. [Abstract] [Full Text] [PDF]

				Z. Pei, G. Reske, Q. Huang, B. D. Hammock, Y. Qi, and N. Chejanovsky Characterization of the Apoptosis Suppressor Protein P49 from the Spodoptera littoralis Nucleopolyhedrovirus J. Biol. Chem., December 6, 2002; 277(50): 48677 - 48684. [Abstract] [Full Text] [PDF]

				M. Kuroyanagi, M. Nishimura, and I. Hara-Nishimura Activation of Arabidopsis Vacuolar Processing Enzyme by Self-Catalytic Removal of an Auto-Inhibitory Domain of the C-Terminal Propeptide Plant Cell Physiol., February 1, 2002; 43(2): 143 - 151. [Abstract] [Full Text] [PDF]

				S.-Y. Yu, S. J. Yoo, L. Yang, C. Zapata, A. Srinivasan, B. A. Hay, and N. E. Baker A pathway of signals regulating effector and initiator caspases in the developing Drosophila eye Development, January 7, 2002; 129(13): 3269 - 3278. [Abstract] [Full Text] [PDF]

				Q. Huang, Q. L. Deveraux, S. Maeda, G. S. Salvesen, H. R. Stennicke, B. D. Hammock, and J. C. Reed Evolutionary conservation of apoptosis mechanisms: Lepidopteran and baculoviral inhibitor of apoptosis proteins are inhibitors of mammalian caspase-9 PNAS, February 15, 2000; 97(4): 1427 - 1432. [Abstract] [Full Text] [PDF]

				S. J. Zoog, J. Bertin, and P. D. Friesen Caspase Inhibition by Baculovirus P35 Requires Interaction between the Reactive Site Loop and the beta -Sheet Core J. Biol. Chem., September 10, 1999; 274(37): 25995 - 26002. [Abstract] [Full Text] [PDF]

				E. A. Prikhod'ko and L. K. Miller The Baculovirus PE38 Protein Augments Apoptosis Induced by Transactivator IE1 J. Virol., August 1, 1999; 73(8): 6691 - 6699. [Abstract] [Full Text]

				N. K. Sah, T. K. Taneja, N. Pathak, R. Begum, M. Athar, and S. E. Hasnain The baculovirus antiapoptotic p35 gene also functions via an oxidant-dependent pathway PNAS, April 27, 1999; 96(9): 4838 - 4843. [Abstract] [Full Text] [PDF]

				G. G. Prikhod'ko, Y. Wang, E. Freulich, C. Prives, and L. K. Miller Baculovirus p33 Binds Human p53 and Enhances p53-Mediated Apoptosis J. Virol., February 1, 1999; 73(2): 1227 - 1234. [Abstract] [Full Text]

				Q. Du, D. Lehavi, O. Faktor, Y. Qi, and N. Chejanovsky Isolation of an Apoptosis Suppressor Gene of the Spodoptera littoralis Nucleopolyhedrovirus J. Virol., February 1, 1999; 73(2): 1278 - 1285. [Abstract] [Full Text]

				J.-C. Lee, H.-H. Chen, and Y.-C. Chao Persistent Baculovirus Infection Results from Deletion of the Apoptotic Suppressor Gene p35 J. Virol., November 1, 1998; 72(11): 9157 - 9165. [Abstract] [Full Text] [PDF]

				V. E. Nava, A. Rosen, M. A. Veliuona, R. J. Clem, B. Levine, and J. M. Hardwick Sindbis Virus Induces Apoptosis through a Caspase-Dependent, CrmA-Sensitive Pathway J. Virol., January 1, 1998; 72(1): 452 - 459. [Abstract] [Full Text] [PDF]

				S. Seshagiri and L. K. Miller Baculovirus inhibitors of apoptosis (IAPs) block activation of Sf-caspase-1 PNAS, December 9, 1997; 94(25): 13606 - 13611. [Abstract] [Full Text] [PDF]

				D. J. LaCount, S. F. Hanson, C. L. Schneider, and P. D. Friesen Caspase Inhibitor P35 and Inhibitor of Apoptosis Op-IAP Block in Vivo Proteolytic Activation of an Effector Caspase at Different Steps J. Biol. Chem., May 19, 2000; 275(21): 15657 - 15664. [Abstract] [Full Text] [PDF]

				C. J. Hawkins, S. J. Yoo, E. P. Peterson, S. L. Wang, S. Y. Vernooy, and B. A. Hay The Drosophila Caspase DRONC Cleaves following Glutamate or Aspartate and Is Regulated by DIAP1, HID, and GRIM J. Biol. Chem., August 25, 2000; 275(35): 27084 - 27093. [Abstract] [Full Text] [PDF]

				G. A. Manji and P. D. Friesen Apoptosis in Motion. AN APICAL, P35-INSENSITIVE CASPASE MEDIATES PROGRAMMED CELL DEATH IN INSECT CELLS J. Biol. Chem., May 11, 2001; 276(20): 16704 - 16710. [Abstract] [Full Text] [PDF]