HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sekine, T. Articles by Endou, H. Search for Related Content PubMed PubMed Citation Articles by Sekine, T. Articles by Endou, H. Social Bookmarking                      What's this?

Volume 272, Number 30, Issue of July 25, 1997 pp. 18526-18529
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

COMMUNICATION:
Expression Cloning and Characterization of a Novel Multispecific Organic Anion Transporter

(Received for publication, April 15, 1997, and in revised form, June 3, 1997)

From the Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181, Japan

Numerous drugs and endogenous compounds are efficiently excreted from the renal proximal tubule via carrier-mediated pathways. Transepithelial excretion of organic anions occurs via their accumulative transport from the blood into the proximal tubule cells across the basolateral membrane and subsequent secretion into the urine through the apical membrane. Here we report on the isolation of a novel complementary DNA from rat kidney that encodes a 551-amino acid residue protein (OAT1) with 12 putative membrane-spanning domains. When expressed in Xenopus laevis oocytes, OAT1 mediated sodium-independent para-aminohippurate (PAH) uptake (K_m = 14.3 ± 2.9 µM). The uptake rate of PAH was increased by the outwardly directed dicarboxylate gradient, consisting with the idea that OAT1 is an organic anion/dicarboxylate exchanger. OAT1 displayed remarkably wide substrate selectivity, covering endogenous substrates such as cyclic nucleotides, a prostaglandin and uric acid, and a variety of drugs with different structures (e.g. antibiotics, a nonsteroidal anti-inflammatory drug, diuretics, an antineoplastic drug, and a uricosuric drug). The Northern blot analysis and in situ hybridization revealed that OAT1 is exclusively expressed in the particular segment of the proximal tubule in the kidney. These data suggest that OAT1 is a multispecific organic anion transporter at the basolateral membrane of the proximal tubule. Isolation of OAT1 will facilitate elucidation of the molecular basis of drug kinetics and the development of new drugs lacking unwanted side effects.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. Duan and G. You Novobiocin Is a Potent Inhibitor for Human Organic Anion Transporters Drug Metab. Dispos., June 1, 2009; 37(6): 1203 - 1210. [Abstract] [Full Text] [PDF]

				K.-i. Hosoya, A. Makihara, Y. Tsujikawa, D. Yoneyama, S. Mori, T. Terasaki, S.-i. Akanuma, M. Tomi, and M. Tachikawa Roles of Inner Blood-Retinal Barrier Organic Anion Transporter 3 in the Vitreous/Retina-to-Blood Efflux Transport of p-Aminohippuric Acid, Benzylpenicillin, and 6-Mercaptopurine J. Pharmacol. Exp. Ther., April 1, 2009; 329(1): 87 - 93. [Abstract] [Full Text] [PDF]

				B. M. Schmitt, D. Gorbunov, P. Schlachtbauer, B. Egenberger, V. Gorboulev, E. Wischmeyer, T. Muller, and H. Koepsell Charge-to-substrate ratio during organic cation uptake by rat OCT2 is voltage dependent and altered by exchange of glutamate 448 with glutamine Am J Physiol Renal Physiol, April 1, 2009; 296(4): F709 - F722. [Abstract] [Full Text] [PDF]

				Q. Zhang, M. Hong, P. Duan, Z. Pan, J. Ma, and G. You Organic Anion Transporter OAT1 Undergoes Constitutive and Protein Kinase C-regulated Trafficking through a Dynamin- and Clathrin-dependent Pathway J. Biol. Chem., November 21, 2008; 283(47): 32570 - 32579. [Abstract] [Full Text] [PDF]

				A. Bahn, Y. Hagos, S. Reuter, D. Balen, H. Brzica, W. Krick, B. C. Burckhardt, I. Sabolic, and G. Burckhardt Identification of a New Urate and High Affinity Nicotinate Transporter, hOAT10 (SLC22A13) J. Biol. Chem., June 13, 2008; 283(24): 16332 - 16341. [Abstract] [Full Text] [PDF]

				T. Matsuzaki, T. Morisaki, W. Sugimoto, K. Yokoo, D. Sato, H. Nonoguchi, K. Tomita, T. Terada, K.-i. Inui, A. Hamada, et al. Altered Pharmacokinetics of Cationic Drugs Caused by Down-Regulation of Renal Rat Organic Cation Transporter 2 (Slc22a2) and Rat Multidrug and Toxin Extrusion 1 (Slc47a1) in Ischemia/Reperfusion-Induced Acute Kidney Injury Drug Metab. Dispos., April 1, 2008; 36(4): 649 - 654. [Abstract] [Full Text] [PDF]

				C. D. Cropp, T. Komori, J. E. Shima, T. J. Urban, S. W. Yee, S. S. More, and K. M. Giacomini Organic Anion Transporter 2 (SLC22A7) Is a Facilitative Transporter of cGMP Mol. Pharmacol., April 1, 2008; 73(4): 1151 - 1158. [Abstract] [Full Text] [PDF]

				S. A. Eraly, V. Vallon, T. Rieg, J. A. Gangoiti, W. R. Wikoff, G. Siuzdak, B. A. Barshop, and S. K. Nigam Multiple organic anion transporters contribute to net renal excretion of uric acid Physiol Genomics, April 1, 2008; 33(2): 180 - 192. [Abstract] [Full Text] [PDF]

				S.-i. Matsumoto, K. Yoshida, N. Ishiguro, T. Maeda, and I. Tamai Involvement of Rat and Human Organic Anion Transporter 3 in the Renal Tubular Secretion of Topotecan [(S)-9-Dimethylaminomethyl-10-hydroxy-camptothecin hydrochloride] J. Pharmacol. Exp. Ther., September 1, 2007; 322(3): 1246 - 1252. [Abstract] [Full Text] [PDF]

				Y. Nozaki, H. Kusuhara, T. Kondo, M. Iwaki, Y. Shiroyanagi, H. Nakayama, S. Horita, H. Nakazawa, T. Okano, and Y. Sugiyama Species Difference in the Inhibitory Effect of Nonsteroidal Anti-Inflammatory Drugs on the Uptake of Methotrexate by Human Kidney Slices J. Pharmacol. Exp. Ther., September 1, 2007; 322(3): 1162 - 1170. [Abstract] [Full Text] [PDF]

				A. S. Windass, S. Lowes, Y. Wang, and C. D. A. Brown The Contribution of Organic Anion Transporters OAT1 and OAT3 to the Renal Uptake of Rosuvastatin J. Pharmacol. Exp. Ther., September 1, 2007; 322(3): 1221 - 1227. [Abstract] [Full Text] [PDF]

				O. Kwon, S.-M. Hong, and K. Blouch Alteration in Renal Organic Anion Transporter 1 After Ischemia/Reperfusion in Cadaveric Renal Allografts J. Histochem. Cytochem., June 1, 2007; 55(6): 575 - 584. [Abstract] [Full Text] [PDF]

				A. N. Rizwan, W. Krick, and G. Burckhardt The Chloride Dependence of the Human Organic Anion Transporter 1 (hOAT1) Is Blunted by Mutation of a Single Amino Acid J. Biol. Chem., May 4, 2007; 282(18): 13402 - 13409. [Abstract] [Full Text] [PDF]

				M. Hong, F. Zhou, K. Lee, and G. You The Putative Transmembrane Segment 7 of Human Organic Anion Transporter hOAT1 Dictates Transporter Substrate Binding and Stability J. Pharmacol. Exp. Ther., March 1, 2007; 320(3): 1209 - 1215. [Abstract] [Full Text] [PDF]

				A. A. K. El-Sheikh, J. J. M. W. van den Heuvel, J. B. Koenderink, and F. G. M. Russel Interaction of Nonsteroidal Anti-Inflammatory Drugs with Multidrug Resistance Protein (MRP) 2/ABCC2- and MRP4/ABCC4-Mediated Methotrexate Transport J. Pharmacol. Exp. Ther., January 1, 2007; 320(1): 229 - 235. [Abstract] [Full Text] [PDF]

				E. K. Jackson, Z. Mi, and R. K. Dubey The Extracellular cAMP-Adenosine Pathway Significantly Contributes to the in Vivo Production of Adenosine J. Pharmacol. Exp. Ther., January 1, 2007; 320(1): 117 - 123. [Abstract] [Full Text] [PDF]

				A. G. Aslamkhan, D. M. Thompson, J. L. Perry, K. Bleasby, N. A. Wolff, S. Barros, D. S. Miller, and J. B. Pritchard The flounder organic anion transporter fOat has sequence, function, and substrate specificity similarity to both mammalian Oat1 and Oat3 Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1773 - R1780. [Abstract] [Full Text] [PDF]

				Y. Hagos, J. Steffgen, A. N. Rizwan, D. Langheit, A. Knoll, G. Burckhardt, and B. C. Burckhardt Functional roles of cationic amino acid residues in the sodium-dicarboxylate cotransporter 3 (NaDC-3) from winter flounder Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1224 - F1231. [Abstract] [Full Text] [PDF]

				W. Xu, K. Tanaka, A.-q. Sun, and G. You Functional Role of the C Terminus of Human Organic Anion Transporter hOAT1 J. Biol. Chem., October 20, 2006; 281(42): 31178 - 31183. [Abstract] [Full Text] [PDF]

				M. Hiasa, T. Matsumoto, T. Komatsu, and Y. Moriyama Wide variety of locations for rodent MATE1, a transporter protein that mediates the final excretion step for toxic organic cations Am J Physiol Cell Physiol, October 1, 2006; 291(4): C678 - C686. [Abstract] [Full Text] [PDF]

				G. W. Schnabolk, G. L. Youngblood, and D. H. Sweet Transport of estrone sulfate by the novel organic anion transporter Oat6 (Slc22a20) Am J Physiol Renal Physiol, August 1, 2006; 291(2): F314 - F321. [Abstract] [Full Text] [PDF]

				K. L. Price, Y. Y. Sautin, D. A. Long, L. Zhang, H. Miyazaki, W. Mu, H. Endou, and R. J. Johnson Human Vascular Smooth Muscle Cells Express a Urate Transporter J. Am. Soc. Nephrol., July 1, 2006; 17(7): 1791 - 1795. [Abstract] [Full Text] [PDF]

				E. K. Jackson, L. C. Zacharia, M. Zhang, D. G. Gillespie, C. Zhu, and R. K. Dubey cAMP-Adenosine Pathway in the Proximal Tubule J. Pharmacol. Exp. Ther., June 1, 2006; 317(3): 1219 - 1229. [Abstract] [Full Text] [PDF]

				N. Bakhiya, M. Stephani, A. Bahn, B. Ugele, A. Seidel, G. Burckhardt, and H. Glatt Uptake of Chemically Reactive, DNA-Damaging Sulfuric Acid Esters into Renal Cells by Human Organic Anion Transporters J. Am. Soc. Nephrol., May 1, 2006; 17(5): 1414 - 1421. [Abstract] [Full Text] [PDF]

				S. A. Eraly, V. Vallon, D. A. Vaughn, J. A. Gangoiti, K. Richter, M. Nagle, J. C. Monte, T. Rieg, D. M. Truong, J. M. Long, et al. Decreased Renal Organic Anion Secretion and Plasma Accumulation of Endogenous Organic Anions in OAT1 Knock-out Mice J. Biol. Chem., February 24, 2006; 281(8): 5072 - 5083. [Abstract] [Full Text] [PDF]

				T. Sekine, H. Miyazaki, and H. Endou Molecular physiology of renal organic anion transporters Am J Physiol Renal Physiol, February 1, 2006; 290(2): F251 - F261. [Abstract] [Full Text] [PDF]

				O. Q. P. Yin, B. Tomlinson, and M. S. S. Chow Variability in Renal Clearance of Substrates for Renal Transporters in Chinese Subjects J. Clin. Pharmacol., February 1, 2006; 46(2): 157 - 163. [Abstract] [Full Text] [PDF]

				A. Clarke, L. A. J. Mur, R. M. Darby, and P. Kenton Harpin modulates the accumulation of salicylic acid by Arabidopsis cells via apoplastic alkalization J. Exp. Bot., December 1, 2005; 56(422): 3129 - 3136. [Abstract] [Full Text] [PDF]

				N. Anzai, P. Jutabha, A. Enomoto, H. Yokoyama, H. Nonoguchi, T. Hirata, K. Shiraya, X. He, S. H. Cha, M. Takeda, et al. Functional Characterization of Rat Organic Anion Transporter 5 (Slc22a19) at the Apical Membrane of Renal Proximal Tubules J. Pharmacol. Exp. Ther., November 1, 2005; 315(2): 534 - 544. [Abstract] [Full Text] [PDF]

				S. Soodvilai, S. H. Wright, W. H. Dantzler, and V. Chatsudthipong Involvement of tyrosine kinase and PI3K in the regulation of OAT3-mediated estrone sulfate transport in isolated rabbit renal proximal tubules Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1057 - F1064. [Abstract] [Full Text] [PDF]

				C. E. Wood, R. Cousins, D. Zhang, and M. Keller-Wood Ontogeny of Expression of Organic Anion Transporters 1 and 3 in Ovine Fetal and Neonatal Kidney Experimental Biology and Medicine, October 1, 2005; 230(9): 668 - 673. [Abstract] [Full Text] [PDF]

				M. Hong, W. Xu, T. Yoshida, K. Tanaka, D. J. Wolff, F. Zhou, M. Inouye, and G. You Human Organic Anion Transporter hOAT1 Forms Homooligomers J. Biol. Chem., September 16, 2005; 280(37): 32285 - 32290. [Abstract] [Full Text] [PDF]

				Y. Kobayashi, A. Shibusawa, H. Saito, N. Ohshiro, M. Ohbayashi, N. Kohyama, and T. Yamamoto Isolation and Functional Characterization of a Novel Organic Solute Carrier Protein, hOSCP1 J. Biol. Chem., September 16, 2005; 280(37): 32332 - 32339. [Abstract] [Full Text] [PDF]

				N. Morita, H. Kusuhara, Y. Nozaki, H. Endou, and Y. Sugiyama FUNCTIONAL INVOLVEMENT OF RAT ORGANIC ANION TRANSPORTER 2 (SLC22A7) IN THE HEPATIC UPTAKE OF THE NONSTEROIDAL ANTI-INFLAMMATORY DRUG KETOPROFEN Drug Metab. Dispos., August 1, 2005; 33(8): 1151 - 1157. [Abstract] [Full Text] [PDF]

				K. Bleasby, L. A. Hall, J. L. Perry, H. W. Mohrenweiser, and J. B. Pritchard Functional Consequences of Single Nucleotide Polymorphisms in the Human Organic Anion Transporter hOAT1 (SLC22A6) J. Pharmacol. Exp. Ther., August 1, 2005; 314(2): 923 - 931. [Abstract] [Full Text] [PDF]

				B. M. Schmitt and H. Koepsell Alkali Cation Binding and Permeation in the Rat Organic Cation Transporter rOCT2 J. Biol. Chem., July 1, 2005; 280(26): 24481 - 24490. [Abstract] [Full Text] [PDF]

				B. C. Burckhardt, J. Lorenz, C. Kobbe, and G. Burckhardt Substrate specificity of the human renal sodium dicarboxylate cotransporter, hNaDC-3, under voltage-clamp conditions Am J Physiol Renal Physiol, April 1, 2005; 288(4): F792 - F799. [Abstract] [Full Text] [PDF]

				L. M. Augustine, R. J. Markelewicz Jr., K. Boekelheide, and N. J. Cherrington XENOBIOTIC AND ENDOBIOTIC TRANSPORTER MRNA EXPRESSION IN THE BLOOD-TESTIS BARRIER Drug Metab. Dispos., January 1, 2005; 33(1): 182 - 189. [Abstract] [Full Text] [PDF]

				P. H.E. Smeets, R. A.M.H. van Aubel, A. C. Wouterse, J. J.M.W. van den Heuvel, and F. G.M. Russel Contribution of Multidrug Resistance Protein 2 (MRP2/ABCC2) to the Renal Excretion of p-aminohippurate (PAH) and Identification of MRP4 (ABCC4) as a Novel PAH Transporter J. Am. Soc. Nephrol., November 1, 2004; 15(11): 2828 - 2835. [Abstract] [Full Text] [PDF]

				X. Zhang, C. E. Groves, A. Bahn, W. M. Barendt, M. D. Prado, M. Rodiger, V. Chatsudthipong, G. Burckhardt, and S. H. Wright Relative contribution of OAT and OCT transporters to organic electrolyte transport in rabbit proximal tubule Am J Physiol Renal Physiol, November 1, 2004; 287(5): F999 - F1010. [Abstract] [Full Text] [PDF]

				S. Soodvilai, V. Chatsudthipong, K. K. Evans, S. H. Wright, and W. H. Dantzler Acute regulation of OAT3-mediated estrone sulfate transport in isolated rabbit renal proximal tubules Am J Physiol Renal Physiol, November 1, 2004; 287(5): F1021 - F1029. [Abstract] [Full Text] [PDF]

				T. Imaoka, H. Kusuhara, S. Adachi-Akahane, M. Hasegawa, N. Morita, H. Endou, and Y. Sugiyama The Renal-Specific Transporter Mediates Facilitative Transport of Organic Anions at the Brush Border Membrane of Mouse Renal Tubules J. Am. Soc. Nephrol., August 1, 2004; 15(8): 2012 - 2022. [Abstract] [Full Text] [PDF]

				G. L. Youngblood and D. H. Sweet Identification and functional assessment of the novel murine organic anion transporter Oat5 (Slc22a19) expressed in kidney Am J Physiol Renal Physiol, August 1, 2004; 287(2): F236 - F244. [Abstract] [Full Text] [PDF]

				S. H. Wright and W. H. Dantzler Molecular and Cellular Physiology of Renal Organic Cation and Anion Transport Physiol Rev, July 1, 2004; 84(3): 987 - 1049. [Abstract] [Full Text] [PDF]

				S. Aruga, A. M. Pajor, K. Nakamura, L. Liu, O. W. Moe, P. A. Preisig, and R. J. Alpern OKP cells express the Na-dicarboxylate cotransporter NaDC-1 Am J Physiol Cell Physiol, July 1, 2004; 287(1): C64 - C72. [Abstract] [Full Text] [PDF]

				M. Ljubojevic, C. M. Herak-Kramberger, Y. Hagos, A. Bahn, H. Endou, G. Burckhardt, and I. Sabolic Rat renal cortical OAT1 and OAT3 exhibit gender differences determined by both androgen stimulation and estrogen inhibition Am J Physiol Renal Physiol, July 1, 2004; 287(1): F124 - F138. [Abstract] [Full Text] [PDF]

				S. A. Eraly, J. C. Monte, and S. K. Nigam Novel slc22 transporter homologs in fly, worm, and human clarify the phylogeny of organic anion and cation transporters Physiol Genomics, June 17, 2004; 18(1): 12 - 24. [Abstract] [Full Text] [PDF]

				S. C. N. Buist and C. D. Klaassen RAT AND MOUSE DIFFERENCES IN GENDER-PREDOMINANT EXPRESSION OF ORGANIC ANION TRANSPORTER (OAT1-3; SLC22A6-8) mRNA LEVELS Drug Metab. Dispos., June 1, 2004; 32(6): 620 - 625. [Abstract] [Full Text] [PDF]

				Y. Kobayashi, N. Ohshiro, A. Tsuchiya, N. Kohyama, M. Ohbayashi, and T. Yamamoto RENAL TRANSPORT OF ORGANIC COMPOUNDS MEDIATED BY MOUSE ORGANIC ANION TRANSPORTER 3 (MOAT3): FURTHER SUBSTRATE SPECIFICITY OF MOAT3 Drug Metab. Dispos., May 1, 2004; 32(5): 479 - 483. [Abstract] [Full Text] [PDF]

				A. Bahn, C. Ebbinghaus, D. Ebbinghaus, E. G. Ponimaskin, L. Fuzesi, G. Burckhardt, and Y. Hagos EXPRESSION STUDIES AND FUNCTIONAL CHARACTERIZATION OF RENAL HUMAN ORGANIC ANION TRANSPORTER 1 ISOFORMS Drug Metab. Dispos., April 1, 2004; 32(4): 424 - 430. [Abstract] [Full Text] [PDF]

				Y. Nozaki, H. Kusuhara, H. Endou, and Y. Sugiyama Quantitative Evaluation of the Drug-Drug Interactions between Methotrexate and Nonsteroidal Anti-Inflammatory Drugs in the Renal Uptake Process Based on the Contribution of Organic Anion Transporters and Reduced Folate Carrier J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 226 - 234. [Abstract] [Full Text]

				C. Sauvant, D. Hesse, H. Holzinger, K. K. Evans, W. H. Dantzler, and M. Gekle Action of EGF and PGE2 on basolateral organic anion uptake in rabbit proximal renal tubules and hOAT1 expressed in human kidney epithelial cells Am J Physiol Renal Physiol, April 1, 2004; 286(4): F774 - F783. [Abstract] [Full Text] [PDF]

				S. A. Eraly, K. T. Bush, R. V. Sampogna, V. Bhatnagar, and S. K. Nigam The Molecular Pharmacology of Organic Anion Transporters: from DNA to FDA? Mol. Pharmacol., March 1, 2004; 65(3): 479 - 487. [Abstract] [Full Text] [PDF]

				C. Kneuer, K. U. Honscha, and W. Honscha Sodium-dependent methotrexate carrier-1 is expressed in rat kidney: cloning and functional characterization Am J Physiol Renal Physiol, March 1, 2004; 286(3): F564 - F571. [Abstract] [Full Text]

				M. Hosoyamada, K. Ichida, A. Enomoto, T. Hosoya, and H. Endou Function and Localization of Urate Transporter 1 in Mouse Kidney J. Am. Soc. Nephrol., February 1, 2004; 15(2): 261 - 268. [Abstract] [Full Text] [PDF]

				N. Shikano, Y. Kanai, K. Kawai, N. Ishikawa, and H. Endou Transport of 99mTc-MAG3 via Rat Renal Organic Anion Transporter 1 J. Nucl. Med., January 1, 2004; 45(1): 80 - 85. [Abstract] [Full Text] [PDF]

				M. D. Blaufox Transport of 99mTc-MAG3 via Rat Renal Organic Anion J. Nucl. Med., January 1, 2004; 45(1): 86 - 88. [Full Text] [PDF]

				C. Sauvant, H. Holzinger, and M. Gekle Short-Term Regulation of Basolateral Organic Anion Uptake in Proximal Tubular Opossum Kidney Cells: Prostaglandin E2 Acts via Receptor-Mediated Activation of Protein Kinase A J. Am. Soc. Nephrol., December 1, 2003; 14(12): 3017 - 3026. [Abstract] [Full Text] [PDF]

				E. Babu, Y. Kanai, A. Chairoungdua, D. K. Kim, Y. Iribe, S. Tangtrongsup, P. Jutabha, Y. Li, N. Ahmed, S. Sakamoto, et al. Identification of a Novel System L Amino Acid Transporter Structurally Distinct from Heterodimeric Amino Acid Transporters J. Biol. Chem., October 31, 2003; 278(44): 43838 - 43845. [Abstract] [Full Text] [PDF]

				E. Beery, P. Middel, A. Bahn, H. S. Willenberg, Y. Hagos, H. Koepsell, S. R. Bornstein, G. A. Muller, G. Burckhardt, and J. Steffgen Molecular Evidence of Organic Ion Transporters in the Rat Adrenal Cortex with Adrenocorticotropin-Regulated Zonal Expression Endocrinology, October 1, 2003; 144(10): 4519 - 4526. [Abstract] [Full Text] [PDF]

				A. Aslamkhan, Y.-H. Han, R. Walden, D. H. Sweet, and J. B. Pritchard Stoichiometry of organic anion/dicarboxylate exchange in membrane vesicles from rat renal cortex and hOAT1-expressing cells Am J Physiol Renal Physiol, October 1, 2003; 285(4): F775 - F783. [Abstract] [Full Text] [PDF]

				N. Mizuno, T. Niwa, Y. Yotsumoto, and Y. Sugiyama Impact of Drug Transporter Studies on Drug Discovery and Development Pharmacol. Rev., September 1, 2003; 55(3): 425 - 461. [Abstract] [Full Text] [PDF]

				G.-H. Kim, K. Y. Na, S.-Y. Kim, K. W. Joo, Y. K. Oh, S.-W. Chae, H. Endou, and J. S. Han Up-regulation of organic anion transporter 1 protein is induced by chronic furosemide or hydrochlorothiazide infusion in rat kidney Nephrol. Dial. Transplant., August 1, 2003; 18(8): 1505 - 1511. [Abstract] [Full Text] [PDF]

				G.-H. Kim, K. Y. Na, S.-Y. Kim, K. W. Joo, Y. K. Oh, S.-W. Chae, H. Endou, and J. S. Han Up-regulation of organic anion transporter 1 protein is induced by chronic furosemide or hydrochlorothiazide infusion in rat kidney Nephrol. Dial. Transplant., August 1, 2003; 18(88): 1505 - 1511. [Abstract] [Full Text]

				P. Jutabha, Y. Kanai, M. Hosoyamada, A. Chairoungdua, D. K. Kim, Y. Iribe, E. Babu, J. Y. Kim, N. Anzai, V. Chatsudthipong, et al. Identification of a Novel Voltage-driven Organic Anion Transporter Present at Apical Membrane of Renal Proximal Tubule J. Biol. Chem., July 18, 2003; 278(30): 27930 - 27938. [Abstract] [Full Text] [PDF]

				R. Kikuchi, H. Kusuhara, D. Sugiyama, and Y. Sugiyama Contribution of Organic Anion Transporter 3 (Slc22a8) to the Elimination of p-Aminohippuric Acid and Benzylpenicillin across the Blood-Brain Barrier J. Pharmacol. Exp. Ther., July 1, 2003; 306(1): 51 - 58. [Abstract] [Full Text] [PDF]

				M. Hasegawa, H. Kusuhara, H. Endou, and Y. Sugiyama Contribution of Organic Anion Transporters to the Renal Uptake of Anionic Compounds and Nucleoside Derivatives in Rat J. Pharmacol. Exp. Ther., June 1, 2003; 305(3): 1087 - 1097. [Abstract] [Full Text] [PDF]

				S. C. N. Buist, N. J. Cherrington, and C. D. Klaassen Endocrine Regulation of Rat Organic Anion Transporters Drug Metab. Dispos., May 1, 2003; 31(5): 559 - 564. [Abstract] [Full Text] [PDF]

				F. Van Bambeke, J.-M. Michot, and P. M. Tulkens Antibiotic efflux pumps in eukaryotic cells: occurrence and impact on antibiotic cellular pharmacokinetics, pharmacodynamics and toxicodynamics J. Antimicrob. Chemother., May 1, 2003; 51(5): 1067 - 1077. [Full Text] [PDF]

				D. H. Sweet, L. M. S. Chan, R. Walden, X.-P. Yang, D. S. Miller, and J. B. Pritchard Organic anion transporter 3 (Slc22a8) is a dicarboxylate exchanger indirectly coupled to the Na+ gradient Am J Physiol Renal Physiol, April 1, 2003; 284(4): F763 - F769. [Abstract] [Full Text] [PDF]

				S. Ohtsuki, T. Takizawa, H. Takanaga, N. Terasaki, T. Kitazawa, M. Sasaki, T. Abe, K.-i. Hosoya, and T. Terasaki In Vitro Study of the Functional Expression of Organic Anion Transporting Polypeptide 3 at Rat Choroid Plexus Epithelial Cells and Its Involvement in the Cerebrospinal Fluid-to-Blood Transport of Estrone-3-Sulfate Mol. Pharmacol., March 1, 2003; 63(3): 532 - 537. [Abstract] [Full Text] [PDF]

				A. G. Aslamkhan, Y.-H. Han, X.-P. Yang, R. K. Zalups, and J. B. Pritchard Human Renal Organic Anion Transporter 1-Dependent Uptake and Toxicity of Mercuric-Thiol Conjugates in Madin-Darby Canine Kidney Cells Mol. Pharmacol., March 1, 2003; 63(3): 590 - 596. [Abstract] [Full Text] [PDF]

				C. E. Groves, L. Munoz, A. Bahn, G. Burckhardt, and S. H. Wright Interaction of Cysteine Conjugates with Human and Rabbit Organic Anion Transporter 1 J. Pharmacol. Exp. Ther., February 1, 2003; 304(2): 560 - 566. [Abstract] [Full Text] [PDF]

				P. T. Ronaldson and R. Bendayan Renal Drug Transport and Drug-Drug Interactions Journal of Pharmacy Practice, December 1, 2002; 15(6): 490 - 503. [Abstract] [PDF]

				Y. Tsutsumi, T. Deguchi, M. Takano, A. Takadate, W. E. Lindup, and M. Otagiri Renal Disposition of a Furan Dicarboxylic Acid and Other Uremic Toxins in the Rat J. Pharmacol. Exp. Ther., November 1, 2002; 303(2): 880 - 887. [Abstract] [Full Text] [PDF]

				A. Bahn, M. Knabe, Y. Hagos, M. Rodiger, S. Godehardt, D. S. Graber-Neufeld, K. K. Evans, G. Burckhardt, and S. H. Wright Interaction of the Metal Chelator 2,3-Dimercapto-1-propanesulfonate with the Rabbit Multispecific Organic Anion Transporter 1 (rbOAT1) Mol. Pharmacol., November 1, 2002; 62(5): 1128 - 1136. [Abstract] [Full Text] [PDF]

				F. Knauf, B. Rogina, Z. Jiang, P. S. Aronson, and S. L. Helfand Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy PNAS, October 29, 2002; 99(22): 14315 - 14319. [Abstract] [Full Text] [PDF]

				A. S. Koh, T. A. Simmons-Willis, J. B. Pritchard, S. M. Grassl, and N. Ballatori Identification of a Mechanism by Which the Methylmercury Antidotes N-Acetylcysteine and Dimercaptopropanesulfonate Enhance Urinary Metal Excretion: Transport by the Renal Organic Anion Transporter-1 Mol. Pharmacol., October 1, 2002; 62(4): 921 - 926. [Abstract] [Full Text] [PDF]

				F. Pizzagalli, B. Hagenbuch, B. Stieger, U. Klenk, G. Folkers, and P. J. Meier Identification of a Novel Human Organic Anion Transporting Polypeptide as a High Affinity Thyroxine Transporter Mol. Endocrinol., October 1, 2002; 16(10): 2283 - 2296. [Abstract] [Full Text] [PDF]

				S. M. Grassl Urate/alpha -ketoglutarate exchange in avian basolateral membrane vesicles Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1144 - C1154. [Abstract] [Full Text] [PDF]

				A. Enomoto, M. F. Wempe, H. Tsuchida, H. J. Shin, S. H. Cha, N. Anzai, A. Goto, A. Sakamoto, T. Niwa, Y. Kanai, et al. Molecular Identification of a Novel Carnitine Transporter Specific to Human Testis. INSIGHTS INTO THE MECHANISM OF CARNITINE RECOGNITION J. Biol. Chem., September 20, 2002; 277(39): 36262 - 36271. [Abstract] [Full Text] [PDF]

				Y. Kato, K. Kuge, H. Kusuhara, P. J. Meier, and Y. Sugiyama Gender Difference in the Urinary Excretion of Organic Anions in Rats J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 483 - 489. [Abstract] [Full Text] [PDF]

				M. Takeda, S. Khamdang, S. Narikawa, H. Kimura, M. Hosoyamada, S. H. Cha, T. Sekine, and H. Endou Characterization of Methotrexate Transport and Its Drug Interactions with Human Organic Anion Transporters J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 666 - 671. [Abstract] [Full Text] [PDF]

				C. Sauvant, H. Holzinger, and M. Gekle Short-Term Regulation of Basolateral Organic Anion Uptake in Proximal Tubular OK cells: EGF Acts via MAPK, PLA2, and COX1 J. Am. Soc. Nephrol., August 1, 2002; 13(8): 1981 - 1991. [Abstract] [Full Text] [PDF]

				D. H. Sweet, D. S. Miller, J. B. Pritchard, Y. Fujiwara, D. R. Beier, and S. K. Nigam Impaired Organic Anion Transport in Kidney and Choroid Plexus of Organic Anion Transporter 3 (Oat3 (Slc22a8)) Knockout Mice J. Biol. Chem., July 19, 2002; 277(30): 26934 - 26943. [Abstract] [Full Text] [PDF]

				Y. Kobayashi, N. Ohshiro, A. Shibusawa, T. Sasaki, S. Tokuyama, T. Sekine, H. Endou, and T. Yamamoto Isolation, Characterization and Differential Gene Expression of Multispecific Organic Anion Transporter 2 in Mice Mol. Pharmacol., July 1, 2002; 62(1): 7 - 14. [Abstract] [Full Text] [PDF]

				A. Enomoto, M. Takeda, A. Tojo, T. Sekine, S. H. Cha, S. Khamdang, F. Takayama, I. Aoyama, S. Nakamura, H. Endou, et al. Role of Organic Anion Transporters in the Tubular Transport of Indoxyl Sulfate and the Induction of its Nephrotoxicity J. Am. Soc. Nephrol., July 1, 2002; 13(7): 1711 - 1720. [Abstract] [Full Text] [PDF]

				J. Alcorn and P. J. McNamara Acyclovir, Ganciclovir, and Zidovudine Transfer into Rat Milk Antimicrob. Agents Chemother., June 1, 2002; 46(6): 1831 - 1836. [Abstract] [Full Text] [PDF]

				A. Enomoto, M. Takeda, M. Shimoda, S. Narikawa, Y. Kobayashi, Y. Kobayashi, T. Yamamoto, T. Sekine, S. H. Cha, T. Niwa, et al. Interaction of Human Organic Anion Transporters 2 and 4 with Organic Anion Transport Inhibitors J. Pharmacol. Exp. Ther., June 1, 2002; 301(3): 797 - 802. [Abstract] [Full Text] [PDF]

				Y. L. Zhao, X. B. Cen, M. Ito, K. Yokoyama, K. Takagi, K. Kitaichi, M. Nadai, M. Ohta, K. Takagi, and T. Hasegawa Shiga-Like Toxin II Derived from Escherichia coli O157:H7 Modifies Renal Handling of Levofloxacin in Rats Antimicrob. Agents Chemother., May 1, 2002; 46(5): 1522 - 1528. [Abstract] [Full Text] [PDF]

				Y. Nagata, H. Kusuhara, H. Endou, and Y. Sugiyama Expression and Functional Characterization of Rat Organic Anion Transporter 3 (rOat3) in the Choroid Plexus Mol. Pharmacol., May 1, 2002; 61(5): 982 - 988. [Abstract] [Full Text] [PDF]

				S. C. N. Buist, N. J. Cherrington, S. Choudhuri, D. P. Hartley, and C. D. Klaassen Gender-Specific and Developmental Influences on the Expression of Rat Organic Anion Transporters J. Pharmacol. Exp. Ther., April 1, 2002; 301(1): 145 - 151. [Abstract] [Full Text] [PDF]

				H. Kimura, M. Takeda, S. Narikawa, A. Enomoto, K. Ichida, and H. Endou Human Organic Anion Transporters and Human Organic Cation Transporters Mediate Renal Transport of Prostaglandins J. Pharmacol. Exp. Ther., April 1, 2002; 301(1): 293 - 298. [Abstract] [Full Text] [PDF]

				R. Kojima, T. Sekine, M. Kawachi, S. H. Cha, Y. Suzuki, and H. Endou Immunolocalization of Multispecific Organic Anion Transporters, OAT1, OAT2, and OAT3, in Rat Kidney J. Am. Soc. Nephrol., April 1, 2002; 13(4): 848 - 857. [Abstract] [Full Text] [PDF]

				H. Motohashi, Y. Sakurai, H. Saito, S. Masuda, Y. Urakami, M. Goto, A. Fukatsu, O. Ogawa, and K.-i. Inui Gene Expression Levels and Immunolocalization of Organic Ion Transporters in the Human Kidney J. Am. Soc. Nephrol., April 1, 2002; 13(4): 866 - 874. [Abstract] [Full Text] [PDF]

				M. Hasegawa, H. Kusuhara, D. Sugiyama, K. Ito, S. Ueda, H. Endou, and Y. Sugiyama Functional Involvement of Rat Organic Anion Transporter 3 (rOat3; Slc22a8) in the Renal Uptake of Organic Anions J. Pharmacol. Exp. Ther., March 1, 2002; 300(3): 746 - 753. [Abstract] [Full Text] [PDF]

				M. Takeda, S. Khamdang, S. Narikawa, H. Kimura, Y. Kobayashi, T. Yamamoto, S. H. Cha, T. Sekine, and H. Endou Human Organic Anion Transporters and Human Organic Cation Transporters Mediate Renal Antiviral Transport J. Pharmacol. Exp. Ther., March 1, 2002; 300(3): 918 - 924. [Abstract] [Full Text] [PDF]

				R. A. M. H. van Aubel, P. H. E. Smeets, J. G. P. Peters, R. J. M. Bindels, and F. G. M. Russel The MRP4/ABCC4 Gene Encodes a Novel Apical Organic Anion Transporter in Human Kidney Proximal Tubules: Putative Efflux Pump for Urinary cAMP and cGMP J. Am. Soc. Nephrol., March 1, 2002; 13(3): 595 - 603. [Abstract] [Full Text] [PDF]

				C. S. Wilcox New Insights into Diuretic Use in Patients with Chronic Renal Disease J. Am. Soc. Nephrol., March 1, 2002; 13(3): 798 - 805. [Abstract] [Full Text] [PDF]

				G. Hill, T. Cihlar, C. Oo, E. S. Ho, K. Prior, H. Wiltshire, J. Barrett, B. Liu, and P. Ward The Anti-Influenza Drug Oseltamivir Exhibits Low Potential to Induce Pharmacokinetic Drug Interactions via Renal Secretion---Correlation of in Vivo and in Vitro Studies Drug Metab. Dispos., January 1, 2002; 30(1): 13 - 19. [Abstract] [Full Text] [PDF]