HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martini, J.-F. Articles by Kelly, P. A. Search for Related Content PubMed PubMed Citation Articles by Martini, J.-F. Articles by Kelly, P. A. Social Bookmarking                      What's this?

Volume 272, Number 30, Issue of July 25, 1997 pp. 18951-18958
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Monkey Growth Hormone (GH) Receptor Gene Expression
EVIDENCE FOR TWO MECHANISMS FOR THE GENERATION OF THE GH BINDING PROTEIN

(Received for publication, January 24, 1997, and in revised form, April 7, 1997)

From INSERM Unité 344, Endocrinologie Moléculaire, Faculté de Médecine Necker-Enfants Malades, 156 rue de Vaugirard, 75730 Paris Cedex 15, France and the ^¶ Département de Physiologie Systémique, CERMA, Base d'Essais en Vol, 91228 Brétigny-sur-Orge Cedex, France

The growth hormone receptor (GHR) cDNA was cloned from the liver of Rhesus macaque using polymerase chain reaction. As deduced from the nucleotide sequence, the mature GHR is a protein of 620 amino acids which presents 94.1% identity with the human receptor. The monkey GHR (mkGHR) expressed in 293 cells presented the expected specificity for a primate GHR and was able to transduce a transcriptional effect of GH. Human GH was able to activate tyrosine phosphorylation of both the tyrosine kinase JAK2 and the receptor in 293 cells co-transfected with mkGHR and JAK2 cDNAs. The GH binding protein (GHBP), the soluble short form of the GHR, was also present in monkey serum. Expression of the GHR cDNA in eucaryotic cells indicated that the GHBP can be produced by proteolytic cleavage of the membrane receptor. Northern blot analysis of GHR gene expression in different tissues allowed us to identify three different transcripts of 5.0 and 2.8 kilobase pairs and a smaller one of 1.7 kilobase pairs which could encode a GHBP. Rapid amplification of cDNA extremities (3-RACE-polymerase chain reaction) was used to identify a cDNA encoding a protein in which the transmembrane and cytoplasmic domains of the receptor are substituted by a short sequence of 9 amino acids. This transcript was present in various tissues and could encode a GHBP as well, suggesting for the first time that two different mechanisms can coexist for the generation of the GHBP: proteolytic cleavage of the membrane receptor and a specific mRNA produced by alternative splicing.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. T.C.C. Patrao, D. B.C. Queiroz, G. Grossman, P. Petrusz, M. d. F. M. Lazari, and M. C. W. Avellar Cloning, expression and immunolocalization of {alpha}1-adrenoceptor in different tissues from rhesus monkey and human male reproductive tract Mol. Hum. Reprod., February 1, 2008; 14(2): 85 - 96. [Abstract] [Full Text] [PDF]

				J. W. Cowan, X. Wang, R. Guan, K. He, J. Jiang, G. Baumann, R. A. Black, M. S. Wolfe, and S. J. Frank Growth Hormone Receptor Is a Target for Presenilin-dependent {gamma}-Secretase Cleavage J. Biol. Chem., May 13, 2005; 280(19): 19331 - 19342. [Abstract] [Full Text] [PDF]

				M. Maamra, M. Bidlingmaier, M.-C. Postel-Vinay, Z. Wu, C. J. Strasburger, and R. J. M. Ross Generation of Human Soluble Leptin Receptor by Proteolytic Cleavage of Membrane-Anchored Receptors Endocrinology, October 1, 2001; 142(10): 4389 - 4393. [Abstract] [Full Text] [PDF]

				R. Guan, Y. Zhang, J. Jiang, C. A. Baumann, R. A. Black, G. Baumann, and S. J. Frank Phorbol Ester- and Growth Factor-Induced Growth Hormone (GH) Receptor Proteolysis and GH-Binding Protein Shedding: Relationship to GH Receptor Down-Regulation Endocrinology, March 1, 2001; 142(3): 1137 - 1147. [Abstract] [Full Text] [PDF]

				H. Favre-Young, F. Dif, F. Roussille, B. A. Demeneix, P. A. Kelly, M. Edery, and A. de Luze Cross-Talk between Signal Transducer and Activator of Transcription (Stat5) and Thyroid Hormone Receptor-{beta} 1 (TR{beta}1) Signaling Pathways Mol. Endocrinol., September 1, 2000; 14(9): 1411 - 1424. [Abstract] [Full Text]

				T. Amit, M. B. H. Youdim, and Z.'e. Hochberg Does Serum Growth Hormone (GH) Binding Protein Reflect Human GH Receptor Function? J. Clin. Endocrinol. Metab., March 1, 2000; 85(3): 927 - 932. [Abstract] [Full Text]

				A. Pezet, H. Favre, P. A. Kelly, and M. Edery Inhibition and Restoration of Prolactin Signal Transduction by Suppressors of Cytokine Signaling J. Biol. Chem., August 27, 1999; 274(35): 24497 - 24502. [Abstract] [Full Text] [PDF]

				S. Ji, R. Guan, S. J. Frank, and J. L. Messina Insulin Inhibits Growth Hormone Signaling via the Growth Hormone Receptor/JAK2/STAT5B Pathway J. Biol. Chem., May 7, 1999; 274(19): 13434 - 13442. [Abstract] [Full Text] [PDF]

				H. Jiang, C. S. Okamura, and M. C. Lucy Isolation and Characterization of a Novel Promoter for the Bovine Growth Hormone Receptor Gene J. Biol. Chem., March 19, 1999; 274(12): 7893 - 7900. [Abstract] [Full Text] [PDF]

				J. LI, R. S. GILMOUR, J. C. SAUNDERS, M. J. DAUNCEY, and A. L. FOWDEN Activation of the adult mode of ovine growth hormone receptor gene expression by cortisol during late fetal development FASEB J, March 1, 1999; 13(3): 545 - 552. [Abstract] [Full Text]

				A. Edens and F. Talamantes Alternative Processing of Growth Hormone Receptor Transcripts Endocr. Rev., October 1, 1998; 19(5): 559 - 582. [Abstract] [Full Text]