| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, April 9, 1997, and in revised form, May 22, 1997)
From the Two NADPH-cytochrome P450 reductase-encoding
cDNAs were isolated from an Arabidopsis cDNA
library by metabolic interference in a Saccharomyces
cerevisiae mutant disrupted for its endogenous cpr1
gene. ATR1 encodes a protein of 692 amino acids, while
ATR2 encodes either a 712-residue protein (ATR2-1), or a
702-residue protein (ATR2-2) depending on the choice of the initiation
codon. Comparative analysis of ATR1 and ATR2-1 indicates 64% amino
acid sequence identity and the absence of conservation in the third base of conserved amino acid codons. The two Arabidopsis
reductases are encoded by distinct genes whose divergence is expected
an early event in angiosperms evolution. A poly(Ser/Thr) stretch reminiscent of a plant chloroplastic targeting signal is present at the
ATR2-1 N-terminal end but absent in ATR1. The cDNA open reading
frames were expressed in yeast. The recombinant polypeptides were found
present in the yeast endoplasmic reticulum membrane and exhibited a
high specific NADPH-cytochrome c reductase activity. To
gain more insight into the respective functions of the two reductases,
the Arabidopsis cDNA encoding cinnamate 4-hydroxylase (CYP73A5) was cloned and co-expressed with ATR1 or ATR2 in yeast. Biochemical characterization of the Arabidopsis
ATR1/CYP73A5 and ATR2-1/CYP73A5 systems demonstrates that the two
distantly related Arabidopsis reductases similarly support
the first oxidative step of the phenylpropanoid general pathway.
Volume 272, Number 31,
Issue of August 1, 1997
pp. 19176-19186
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
,§
,
and
Centre de Génétique
Moléculaire du CNRS, 91198 Gif-sur-Yvette, France, the
§ Centre Orsan de Recherches en Biotechnologies, 91953 Les Ulis, France, and the ¶ Service de Radiophysiologie
Végétale du CEA,
13108 St. Paul-Lès-Durance, France
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Rontein, S. Onillon, G. Herbette, A. Lesot, D. Werck-Reichhart, C. Sallaud, and A. Tissier CYP725A4 from Yew Catalyzes Complex Structural Rearrangement of Taxa-4(5),11(12)-diene into the Cyclic Ether 5(12)-Oxa-3(11)-cyclotaxane J. Biol. Chem., March 7, 2008; 283(10): 6067 - 6075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takahashi, Y.-S. Yeo, Y. Zhao, P. E. O'Maille, B. T. Greenhagen, J. P. Noel, R. M. Coates, and J. Chappell Functional Characterization of Premnaspirodiene Oxygenase, a Cytochrome P450 Catalyzing Regio- and Stereo-specific Hydroxylations of Diverse Sesquiterpene Substrates J. Biol. Chem., October 26, 2007; 282(43): 31744 - 31754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, S. Subramanian, Y. Zhang, and O. Yu Flavone Synthases from Medicago truncatula Are Flavanone-2-Hydroxylases and Are Important for Nodulation Plant Physiology, June 1, 2007; 144(2): 741 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nagai, M. Koide, S. Takahashi, A. Kikuta, M. Aono, Y. Sasaki-Sekimoto, H. Ohta, K.-i. Takamiya, and T. Masuda Induction of Isoforms of Tetrapyrrole Biosynthetic Enzymes, AtHEMA2 and AtFC1, under Stress Conditions and Their Physiological Functions in Arabidopsis Plant Physiology, June 1, 2007; 144(2): 1039 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-W. Kim, J.-Y. Hwang, Y.-S. Kim, S.-H. Joo, S. C. Chang, J. S. Lee, S. Takatsuto, and S.-K. Kim Arabidopsis CYP85A2, a Cytochrome P450, Mediates the Baeyer-Villiger Oxidation of Castasterone to Brassinolide in Brassinosteroid Biosynthesis PLANT CELL, August 1, 2005; 17(8): 2397 - 2412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ralston, S. Subramanian, M. Matsuno, and O. Yu Partial Reconstruction of Flavonoid and Isoflavonoid Biosynthesis in Yeast Using Soybean Type I and Type II Chalcone Isomerases Plant Physiology, April 1, 2005; 137(4): 1375 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takahashi, Y. Zhao, P. E. O'Maille, B. T. Greenhagen, J. P. Noel, R. M. Coates, and J. Chappell Kinetic and Molecular Analysis of 5-Epiaristolochene 1,3-Dihydroxylase, a Cytochrome P450 Enzyme Catalyzing Successive Hydroxylations of Sesquiterpenes J. Biol. Chem., February 4, 2005; 280(5): 3686 - 3696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aharoni, A. P. Giri, F. W.A. Verstappen, C. M. Bertea, R. Sevenier, Z. Sun, M. A. Jongsma, W. Schwab, and H. J. Bouwmeester Gain and Loss of Fruit Flavor Compounds Produced by Wild and Cultivated Strawberry Species PLANT CELL, November 1, 2004; 16(11): 3110 - 3131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takei, T. Yamaya, and H. Sakakibara Arabidopsis CYP735A1 and CYP735A2 Encode Cytokinin Hydroxylases That Catalyze the Biosynthesis of trans-Zeatin J. Biol. Chem., October 1, 2004; 279(40): 41866 - 41872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rohde, K. Morreel, J. Ralph, G. Goeminne, V. Hostyn, R. De Rycke, S. Kushnir, J. Van Doorsselaere, J.-P. Joseleau, M. Vuylsteke, et al. Molecular Phenotyping of the pal1 and pal2 Mutants of Arabidopsis thaliana Reveals Far-Reaching Consequences on Phenylpropanoid, Amino Acid, and Carbohydrate Metabolism PLANT CELL, October 1, 2004; 16(10): 2749 - 2771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-W. Kim, S. C. Chang, J. S. Lee, S. Takatsuto, T. Yokota, and S.-K. Kim Novel Biosynthetic Pathway of Castasterone from Cholesterol in Tomato Plant Physiology, July 1, 2004; 135(3): 1231 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Malonek, M. C. Rojas, P. Hedden, P. Gaskin, P. Hopkins, and B. Tudzynski The NADPH-cytochrome P450 Reductase Gene from Gibberella fujikuroi Is Essential for Gibberellin Biosynthesis J. Biol. Chem., June 11, 2004; 279(24): 25075 - 25084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Davidson, J. J. Smith, C. A. Helliwell, A. T. Poole, and J. B. Reid The Pea Gene LH Encodes ent-Kaurene Oxidase Plant Physiology, March 1, 2004; 134(3): 1123 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Hong, M. Ueguchi-Tanaka, K. Umemura, S. Uozu, S. Fujioka, S. Takatsuto, S. Yoshida, M. Ashikari, H. Kitano, and M. Matsuoka A Rice Brassinosteroid-Deficient Mutant, ebisu dwarf (d2), Is Caused by a Loss of Function of a New Member of Cytochrome P450 PLANT CELL, December 1, 2003; 15(12): 2900 - 2910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Raes, A. Rohde, J. H. Christensen, Y. Van de Peer, and W. Boerjan Genome-Wide Characterization of the Lignification Toolbox in Arabidopsis Plant Physiology, November 1, 2003; 133(3): 1051 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rupasinghe, J. Baudry, and M. A. Schuler Common active site architecture and binding strategy of four phenylpropanoid P450s from Arabidopsis thaliana as revealed by molecular modeling Protein Eng. Des. Sel., October 1, 2003; 16(10): 721 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shimada, H. Goda, A. Nakamura, S. Takatsuto, S. Fujioka, and S. Yoshida Organ-Specific Expression of Brassinosteroid-Biosynthetic Genes and Distribution of Endogenous Brassinosteroids in Arabidopsis Plant Physiology, January 1, 2003; 131(1): 287 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Davidson, R. C. Elliott, C. A. Helliwell, A. T. Poole, and J. B. Reid The Pea Gene NA Encodes ent-Kaurenoic Acid Oxidase Plant Physiology, January 1, 2003; 131(1): 335 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-K. Ro, J. Ehlting, and C. J. Douglas Cloning, Functional Expression, and Subcellular Localization of Multiple NADPH-Cytochrome P450 Reductases from Hybrid Poplar Plant Physiology, December 1, 2002; 130(4): 1837 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Nair, Q. Xia, C. J. Kartha, E. Kurylo, R. N. Hirji, R. Datla, and G. Selvaraj Arabidopsis CYP98A3 Mediating Aromatic 3-Hydroxylation. Developmental Regulation of the Gene, and Expression in Yeast Plant Physiology, September 1, 2002; 130(1): 210 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Vranova, D. Inze, and F. Van Breusegem Signal transduction during oxidative stress J. Exp. Bot., May 15, 2002; 53(372): 1227 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. W. Watson, J. E. Froehlich, C. A. Josefsson, C. Chapple, F. Durst, I. Benveniste, and R. C. Coolbaugh Localization of CYP86B1 in the Outer Envelope of Chloroplasts Plant Cell Physiol., August 1, 2001; 42(8): 873 - 878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Wellesen, F. Durst, F. Pinot, I. Benveniste, K. Nettesheim, E. Wisman, S. Steiner-Lange, H. Saedler, and A. Yephremov Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid omega -hydroxylation in development PNAS, August 1, 2001; (2001) 171285998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shimada, S. Fujioka, N. Miyauchi, M. Kushiro, S. Takatsuto, T. Nomura, T. Yokota, Y. Kamiya, G. J. Bishop, and S. Yoshida Brassinosteroid-6-Oxidases from Arabidopsis and Tomato Catalyze Multiple C-6 Oxidations in Brassinosteroid Biosynthesis Plant Physiology, June 1, 2001; 126(2): 770 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Ro, N. Mah, B. E. Ellis, and C. J. Douglas Functional Characterization and Subcellular Localization of Poplar (Populus trichocarpa x Populus deltoides) Cinnamate 4-Hydroxylase Plant Physiology, May 1, 2001; 126(1): 317 - 329. [Abstract] [Full Text]
|
|
|
|
|
|
C. A. Helliwell, P. M. Chandler, A. Poole, E. S. Dennis, and W. J. Peacock The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway PNAS, February 1, 2001; (2001) 41588998. [Abstract] [Full Text]
|
|
|
|
|
|
S. Bak, C. E. Olsen, B. A. Halkier, and B. L. Møller Transgenic Tobacco and Arabidopsis Plants Expressing the Two Multifunctional Sorghum Cytochrome P450 Enzymes, CYP79A1 and CYP71E1, Are Cyanogenic and Accumulate Metabolites Derived from Intermediates in Dhurrin Biosynthesis Plant Physiology, August 1, 2000; 123(4): 1437 - 1448. [Abstract] [Full Text]
|
|
|
|
|
|
E. Koopmann, E. Logemann, and K. Hahlbrock Regulation and Functional Expression of Cinnamate 4-Hydroxylase from Parsley Plant Physiology, January 1, 1999; 119(1): 49 - 56. [Abstract] [Full Text]
|
|
|
|
|
|
T. Robineau, Y. Batard, S. Nedelkina, F. Cabello-Hurtado, M. LeRet, O. Sorokine, L. Didierjean, and D. Werck-Reichhart The Chemically Inducible Plant Cytochrome P450 CYP76B1 Actively Metabolizes Phenylureas and Other Xenobiotics Plant Physiology, November 1, 1998; 118(3): 1049 - 1056. [Abstract] [Full Text]
|
|
|
|
|
|
V. Lagree, I. Pellerin, J.-F. Hubert, F. Tacnet, F. Le Caherec, N. Roudier, D. Thomas, J. Gouranton, and S. Deschamps A Yeast Recombinant Aquaporin Mutant That Is Not Expressed or Mistargeted in Xenopus Oocyte Can Be Functionally Analyzed in Reconstituted Proteoliposomes J. Biol. Chem., May 15, 1998; 273(20): 12422 - 12426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Cabello-Hurtado, Y. Batard, J.-P. Salaun, F. Durst, F. Pinot, and D. Werck-Reichhart Cloning, Expression in Yeast, and Functional Characterization of CYP81B1, a Plant Cytochrome P450 That Catalyzes In-chain Hydroxylation of Fatty Acids J. Biol. Chem., March 27, 1998; 273(13): 7260 - 7267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Koopmann and K. Hahlbrock Differentially regulated NADPH:cytochrome P450 oxidoreductases in parsley PNAS, December 23, 1997; 94(26): 14954 - 14959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kitazume, N. Takaya, N. Nakayama, and H. Shoun Fusarium oxysporum Fatty-acid Subterminal Hydroxylase (CYP505) Is a Membrane-bound Eukaryotic Counterpart of Bacillus megaterium Cytochrome P450BM3 J. Biol. Chem., December 8, 2000; 275(50): 39734 - 39740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. O. Latunde-Dada, F. Cabello-Hurtado, N. Czittrich, L. Didierjean, C. Schopfer, N. Hertkorn, D. Werck-Reichhart, and J. Ebel Flavonoid 6-Hydroxylase from Soybean (Glycine max L.), a Novel Plant P-450 Monooxygenase J. Biol. Chem., January 12, 2001; 276(3): 1688 - 1695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Helliwell, P. M. Chandler, A. Poole, E. S. Dennis, and W. J. Peacock The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway PNAS, February 13, 2001; 98(4): 2065 - 2070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wellesen, F. Durst, F. Pinot, I. Benveniste, K. Nettesheim, E. Wisman, S. Steiner-Lange, H. Saedler, and A. Yephremov Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid omega -hydroxylation in development PNAS, August 14, 2001; 98(17): 9694 - 9699. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
