JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, C.
Right arrow Articles by Zhan, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, C.
Right arrow Articles by Zhan, X.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 272, Number 31, Issue of August 1, 1997 pp. 19248-19252
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Proteolysis of Platelet Cortactin by Calpain

(Received for publication, March 4, 1997, and in revised form, May 5, 1997)

Cai Huang Dagger , Narendra N. Tandon § , Nicholas J. Greco § , Yansong Ni Dagger , Tony Wang and Xi Zhan Dagger

From the Dagger  Department of Experimental Pathology and the § Department of Platelet Biology, The Holland Laboratory, American Red Cross, Rockville, MD 20855, the  Division of Biology, Glaxo and Wellcome Research Institute, Research Triangle Park, North Carolina 27709, and the  Department of Anatomy and Cell Biology, George Washington University, Washington, D. C. 20037

Cortactin, a substrate of pp60c-src and a potent filamentous actin binding and cross-linking protein, is abundant in circulating platelets. After stimulation of platelet aggregation with collagen, cortactin undergoes a dramatic increase in tyrosine phosphorylation followed by a rapid degradation. The cleavage of platelet cortactin was detected in lysates prepared using either Triton-containing buffer or SDS-sample buffer. However, the degradation of cortactin was not observed in platelets derived from a Glanzmann's patient, who lacked functional integrin alpha IIbbeta 3 (GPIIb-IIIa). In addition, the proteolysis of cortactin was abolished by treating platelets before but not after collagen stimulation with EGTA or calpeptin. Furthermore, recombinant cortactin was digested by µ-calpain in vitro in a dose-dependent manner, indicating that cortactin is a substrate for calpain. We also observed that the calpain-mediated digestion in vitro is dependent on the presence of a sequence containing a proline-rich region and multiple tyrosine residues that are phosphorylated by pp60c-src. Tyrosine phosphorylation by pp60c-src up-regulates the activity of calpain toward cortactin. Our data suggest that the calpain-mediated proteolysis of tyrosine-phosphorylated cortactin may provide a mechanism to remodel irreversibly the cytoskeleton in response to platelet agonists.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
C. Badowski, G. Pawlak, A. Grichine, A. Chabadel, C. Oddou, P. Jurdic, M. Pfaff, C. Albiges-Rizo, and M. R. Block
Paxillin Phosphorylation Controls Invadopodia/Podosomes Spatiotemporal Organization
Mol. Biol. Cell, February 1, 2008; 19(2): 633 - 645.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
O. Destaing, A. Sanjay, C. Itzstein, W. C. Horne, D. Toomre, P. De Camilli, and R. Baron
The Tyrosine Kinase Activity of c-Src Regulates Actin Dynamics and Organization of Podosomes in Osteoclasts
Mol. Biol. Cell, January 1, 2008; 19(1): 394 - 404.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Luxenburg, J. T. Parsons, L. Addadi, and B. Geiger
Involvement of the Src-cortactin pathway in podosome formation and turnover during polarization of cultured osteoclasts
J. Cell Sci., December 1, 2006; 119(23): 4878 - 4888.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
L. I. Cosen-Binker and A. Kapus
Cortactin: The Gray Eminence of the Cytoskeleton.
Physiology, October 1, 2006; 21(5): 352 - 361.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
B. J. Perrin, K. J. Amann, and A. Huttenlocher
Proteolysis of Cortactin by Calpain Regulates Membrane Protrusion during Cell Migration
Mol. Biol. Cell, January 1, 2006; 17(1): 239 - 250.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. J. Franco and A. Huttenlocher
Regulating cell migration: calpains make the cut
J. Cell Sci., September 1, 2005; 118(17): 3829 - 3838.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Heiska and O. Carpen
Src Phosphorylates Ezrin at Tyrosine 477 and Induces a Phosphospecific Association between Ezrin and a Kelch-Repeat Protein Family Member
J. Biol. Chem., March 18, 2005; 280(11): 10244 - 10252.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Zamorano, M. D. Rivas, F. Setien, and M. Perez-G
Proteolytic Regulation of Activated STAT6 by Calpains
J. Immunol., March 1, 2005; 174(5): 2843 - 2848.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. A. Westhoff, B. Serrels, V. J. Fincham, M. C. Frame, and N. O. Carragher
Src-Mediated Phosphorylation of Focal Adhesion Kinase Couples Actin and Adhesion Dynamics to Survival Signaling
Mol. Cell. Biol., September 15, 2004; 24(18): 8113 - 8133.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kulkarni and S. P. Jackson
Platelet Factor XIII and Calpain Negatively Regulate Integrin {alpha}IIb{beta}3 Adhesive Function and Thrombus Growth
J. Biol. Chem., July 16, 2004; 279(29): 30697 - 30706.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. Knoll and U. Drescher
Src Family Kinases Are Involved in EphA Receptor-Mediated Retinal Axon Guidance
J. Neurosci., July 14, 2004; 24(28): 6248 - 6257.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Martinez-Quiles, H.-Y. H. Ho, M. W. Kirschner, N. Ramesh, and R. S. Geha
Erk/Src Phosphorylation of Cortactin Acts as a Switch On-Switch Off Mechanism That Controls Its Ability To Activate N-WASP
Mol. Cell. Biol., June 15, 2004; 24(12): 5269 - 5280.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Forsythe and A. D. Befus
Inhibition of Calpain Is a Component of Nitric Oxide-Induced Down-Regulation of Human Mast Cell Adhesion
J. Immunol., January 1, 2003; 170(1): 287 - 293.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Hattan, E. Nesti, T. G. Cachero, and A. D. Morielli
Tyrosine Phosphorylation of Kv1.2 Modulates Its Interaction with the Actin-binding Protein Cortactin
J. Biol. Chem., October 4, 2002; 277(41): 38596 - 38606.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
A. Robert, M.-J. Miron, C. Champagne, M.-C. Gingras, P. E. Branton, and J. N. Lavoie
Distinct cell death pathways triggered by the adenovirus early region 4 ORF 4 protein
J. Cell Biol., August 5, 2002; 158(3): 519 - 528.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. S. Lombardi, A. Kavelaars, P. Penela, E. J. Scholtens, M. Roccio, R. E. Schmidt, M. Schedlowski, F. Mayor Jr., and C. J. Heijnen
Oxidative Stress Decreases G Protein-Coupled Receptor Kinase 2 in Lymphocytes via a Calpain-Dependent Mechanism
Mol. Pharmacol., August 1, 2002; 62(2): 379 - 388.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Nicolas, C. M. Fournier, C. Galand, L. Malbert-Colas, O. Bournier, Y. Kroviarski, M. Bourgeois, J. H. Camonis, D. Dhermy, B. Grandchamp, et al.
Tyrosine Phosphorylation Regulates Alpha II Spectrin Cleavage by Calpain
Mol. Cell. Biol., May 15, 2002; 22(10): 3527 - 3536.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. M. Gilligan, R. Sarid, and J. Weese
Adducin in platelets: activation-induced phosphorylation by PKC and proteolysis by calpain
Blood, April 1, 2002; 99(7): 2418 - 2426.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
Bialkowska, Kulkarni, Du, D. Goll, T. Saido, and J. Fox
Evidence that {beta}3 Integrin-induced Rac Activation Involves the Calpain-dependent Formation of Integrin Clusters that Are Distinct from the Focal Complexes and Focal Adhesions that Form as Rac and RhoA become Active
J. Cell Biol., October 30, 2000; 151(3): 685 - 696.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kulkarni, T. C. Saido, K. Suzuki, and J. E. B. Fox
Calpain Mediates Integrin-induced Signaling at a Point Upstream of Rho Family Members
J. Biol. Chem., July 23, 1999; 274(30): 21265 - 21275.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Wu, D. Dowbenko, and L. A. Lasky
PSTPIP 2, a Second Tyrosine Phosphorylated, Cytoskeletal-associated Protein That Binds a PEST-type Protein-tyrosine Phosphatase
J. Biol. Chem., November 13, 1998; 273(46): 30487 - 30496.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Huang, J. Liu, C. C. Haudenschild, and X. Zhan
The Role of Tyrosine Phosphorylation of Cortactin in the Locomotion of Endothelial Cells
J. Biol. Chem., October 2, 1998; 273(40): 25770 - 25776.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kapus, C. Di Ciano, J. Sun, X. Zhan, L. Kim, T. W. Wong, and O. D. Rotstein
Cell Volume-dependent Phosphorylation of Proteins of the Cortical Cytoskeleton and Cell-Cell Contact Sites. THE ROLE OF Fyn AND FER KINASES
J. Biol. Chem., October 6, 2000; 275(41): 32289 - 32298.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. O. Carragher, V. J. Fincham, D. Riley, and M. C. Frame
Cleavage of Focal Adhesion Kinase by Different Proteases during Src-regulated Transformation and Apoptosis. DISTINCT ROLES FOR CALPAIN AND CASPASES
J. Biol. Chem., February 2, 2001; 276(6): 4270 - 4275.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Xu, R. Zhao, Y. Peng, A. Guerrah, and Z. J. Zhao
Association of Tyrosine Phosphatase SHP-2 with F-actin at Low Cell Densities
J. Biol. Chem., July 27, 2001; 276(31): 29479 - 29484.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.