|
Volume 272, Number 31,
Issue of August 1, 1997
pp. 19502-19508
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
Functional Analysis of Glycosyltransferases Encoded by the
Capsular Polysaccharide Biosynthesis Locus of Streptococcus
pneumoniae Serotype 14
(Received for publication, April 21, 1997)
Marc A. B.
Kolkman
,
Bernard A. M.
van der Zeijst
and
Piet J. M.
Nuijten
From the Department of Bacteriology, Institute of Infectious
Diseases and Immunology, School of Veterinary Medicine, University of
Utrecht, 3508 TD Utrecht, The Netherlands.
Bacteria belonging to the species
Streptococcus pneumoniae vary in their capsule. Presently,
90 capsular serotypes are known, all possessing their own specific
polysaccharide structure. Little is known about the biosynthesis of
these capsular polysaccharides. The cps locus of S. pneumoniae serotype 14 was cloned. So far, 7 open reading frames
have been sequenced, cps14B to cps14H. The gene
products are similar to proteins involved in bacterial polysaccharide biosynthesis, both of Gram-negative and -positive micro-organisms. Gene-specific mutants were created for cps14D to
cps14H by insertional mutagenesis. All mutants no longer
agglutinated with a monoclonal antibody against type 14 capsule
polysaccharides. The biosynthetic function of cps14E and
cps14G was determined by analysis of the intermediates in
the synthesis of the oligosaccharide subunit, formed in membrane
preparations of the wild-type and mutant strains and in membrane
preparations of Escherichia coli expressing the pneumococcal glycosyltransferases. The enzyme encoded by
cps14E is a glucosyl-1-phosphate transferase that links
glucose to a lipid carrier, the first step in the biosynthesis of the
type 14 repeating unit. The gene product of cps14G encodes
a -1,4-galactosyltransferase, the enzyme responsible for the second
step in the subunit synthesis, the transfer of galactose to
lipid-linked glucose.

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
Y. Yoshida, J. Yang, P.-E. Peaker, H. Kato, C. A. Bush, and J. O. Cisar
Molecular and Antigenic Characterization of a Streptococcus oralis Coaggregation Receptor Polysaccharide by Carbohydrate Engineering in Streptococcus gordonii
J. Biol. Chem.,
May 2, 2008;
283(18):
12654 - 12664.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Mavroidi, D. M. Aanensen, D. Godoy, I. C. Skovsted, M. S. Kaltoft, P. R. Reeves, S. D. Bentley, and B. G. Spratt
Genetic Relatedness of the Streptococcus pneumoniae Capsular Biosynthetic Loci
J. Bacteriol.,
November 1, 2007;
189(21):
7841 - 7855.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. M. Aanensen, A. Mavroidi, S. D. Bentley, P. R. Reeves, and B. G. Spratt
Predicted Functions and Linkage Specificities of the Products of the Streptococcus pneumoniae Capsular Biosynthetic Loci
J. Bacteriol.,
November 1, 2007;
189(21):
7856 - 7876.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Steiner, R. Novotny, K. Patel, E. Vinogradov, C. Whitfield, M. A. Valvano, P. Messner, and C. Schaffer
Functional Characterization of the Initiation Enzyme of S-Layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus NRS 2004/3a
J. Bacteriol.,
April 1, 2007;
189(7):
2590 - 2598.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Z. Minic, C. Marie, C. Delorme, J.-M. Faurie, G. Mercier, D. Ehrlich, and P. Renault
Control of EpsE, the Phosphoglycosyltransferase Initiating Exopolysaccharide Synthesis in Streptococcus thermophilus, by EpsD Tyrosine Kinase
J. Bacteriol.,
February 15, 2007;
189(4):
1351 - 1357.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. T. Cartee, W. T. Forsee, M. H. Bender, K. D. Ambrose, and J. Yother
CpsE from Type 2 Streptococcus pneumoniae Catalyzes the Reversible Addition of Glucose-1-Phosphate to a Polyprenyl Phosphate Acceptor, Initiating Type 2 Capsule Repeat Unit Formation
J. Bacteriol.,
November 1, 2005;
187(21):
7425 - 7433.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Bickel, L. Lehle, M. Schwarz, M. Aebi, and C. A. Jakob
Biosynthesis of Lipid-linked Oligosaccharides in Saccharomyces cerevisiae: Alg13p AND Alg14p FORM A COMPLEX REQUIRED FOR THE FORMATION OF GlcNAc2-PP-DOLICHOL
J. Biol. Chem.,
October 14, 2005;
280(41):
34500 - 34506.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. A. Videira, A. P. Garcia, and I. Sa-Correia
Functional and Topological Analysis of the Burkholderia cenocepacia Priming Glucosyltransferase BceB, Involved in the Biosynthesis of the Cepacian Exopolysaccharide
J. Bacteriol.,
July 15, 2005;
187(14):
5013 - 5018.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. J. Cieslewicz, D. Chaffin, G. Glusman, D. Kasper, A. Madan, S. Rodrigues, J. Fahey, M. R. Wessels, and C. E. Rubens
Structural and Genetic Diversity of Group B Streptococcus Capsular Polysaccharides
Infect. Immun.,
May 1, 2005;
73(5):
3096 - 3103.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
I. Chantret, J. Dancourt, A. Barbat, and S. E. H. Moore
Two Proteins Homologous to the N- and C-terminal Domains of the Bacterial Glycosyltransferase Murg Are Required for the Second Step of Dolichyl-linked Oligosaccharide Synthesis in Saccharomyces cerevisiae
J. Biol. Chem.,
March 11, 2005;
280(10):
9236 - 9242.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D.-Q. Xu, J. Thompson, and J. O. Cisar
Genetic Loci for Coaggregation Receptor Polysaccharide Biosynthesis in Streptococcus gordonii 38
J. Bacteriol.,
September 15, 2003;
185(18):
5419 - 5430.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. D. Waite, D. W. Penfold, J. K. Struthers, and C. G. Dowson
Spontaneous sequence duplications within capsule genes cap8E and tts control phase variation in Streptococcus pneumoniae serotypes 8 and 37
Microbiology,
February 1, 2003;
149(2):
497 - 504.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. R. Broadbent, D. J. McMahon, D. L. Welker, C. J. Oberg, and S. Moineau
Biochemistry, Genetics, and Applications of Exopolysaccharide Production in Streptococcus thermophilus: A Review
J Dairy Sci,
February 1, 2003;
86(2):
407 - 423.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. van Selm, M. A. B. Kolkman, B. A. M. van der Zeijst, K. A. Zwaagstra, W. Gaastra, and J. P. M. van Putten
Organization and characterization of the capsule biosynthesis locus of Streptococcus pneumoniae serotype 9V
Microbiology,
June 1, 2002;
148(6):
1747 - 1755.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Jolly, J. Newell, I. Porcelli, S. J.F. Vincent, and F. Stingele
Lactobacillus helveticus glycosyltransferases: from genes to carbohydrate synthesis
Glycobiology,
May 1, 2002;
12(5):
319 - 327.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Wang, W. Qu, and P. R. Reeves
Sequence Analysis of Four Shigella boydii O-Antigen Loci: Implication for Escherichia coli and Shigella Relationships
Infect. Immun.,
November 1, 2001;
69(11):
6923 - 6930.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S.-M. Jiang, L. Wang, and P. R. Reeves
Molecular Characterization of Streptococcus pneumoniae Type 4, 6B, 8, and 18C Capsular Polysaccharide Gene Clusters
Infect. Immun.,
March 1, 2001;
69(3):
1244 - 1255.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
E. Almirón-Roig, F. Mulholland, M. J. Gasson, and A. M. Griffin
The complete cps gene cluster from Streptococcus thermophilus NCFB 2393 involved in the biosynthesis of a new exopolysaccharide
Microbiology,
November 1, 2000;
146(11):
2793 - 2802.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
D. O. Chaffin, S. B. Beres, H. H. Yim, and C. E. Rubens
The Serotype of Type Ia and III Group B Streptococci Is Determined by the Polymerase Gene within the Polycistronic Capsule Operon
J. Bacteriol.,
August 15, 2000;
182(16):
4466 - 4477.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
C. Gilbert, K. Robinson, R. W. F. Le Page, and J. M. Wells
Heterologous Expression of an Immunogenic Pneumococcal Type 3 Capsular Polysaccharide in Lactococcus lactis
Infect. Immun.,
June 1, 2000;
68(6):
3251 - 3260.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. G. Hardy, M. J. Caimano, and J. Yother
Capsule Biosynthesis and Basic Metabolism in Streptococcus pneumoniae Are Linked through the Cellular Phosphoglucomutase
J. Bacteriol.,
April 1, 2000;
182(7):
1854 - 1863.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
R. T. Cartee, W. T. Forsee, J. S. Schutzbach, and J. Yother
Mechanism of Type 3 Capsular Polysaccharide Synthesis in Streptococcus pneumoniae
J. Biol. Chem.,
February 11, 2000;
275(6):
3907 - 3914.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. van Kranenburg, H. R. Vos, I. I. van Swam, M. Kleerebezem, and W. M. de Vos
Functional Analysis of Glycosyltransferase Genes from Lactococcus lactis and Other Gram-Positive Cocci: Complementation, Expression, and Diversity
J. Bacteriol.,
October 15, 1999;
181(20):
6347 - 6353.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
R. Muñoz, M. Mollerach, R. López, and E. García
Characterization of the Type 8 Capsular Gene Cluster of Streptococcus pneumoniae
J. Bacteriol.,
October 1, 1999;
181(19):
6214 - 6219.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
H. E. Smith, V. Veenbergen, J. van der Velde, M. Damman, H. J. Wisselink, and M. A. Smits
The cps Genes of Streptococcus suis Serotypes 1, 2, and 9: Development of Rapid Serotype-Specific PCR Assays
J. Clin. Microbiol.,
October 1, 1999;
37(10):
3146 - 3152.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
S. Yamamoto, K. Miyake, Y. Koike, M. Watanabe, Y. Machida, M. Ohta, and S. Iijima
Molecular Characterization of Type-Specific Capsular Polysaccharide Biosynthesis Genes of Streptococcus agalactiae Type Ia
J. Bacteriol.,
September 1, 1999;
181(17):
5176 - 5184.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
J. K. Morona, R. Morona, and J. C. Paton
Analysis of the 5' Portion of the Type 19A Capsule Locus Identifies Two Classes of cpsC, cpsD, and cpsE Genes in Streptococcus pneumoniae
J. Bacteriol.,
June 1, 1999;
181(11):
3599 - 3605.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
F. Iannelli, B. J. Pearce, and G. Pozzi
The Type 2 Capsule Locus of Streptococcus pneumoniae
J. Bacteriol.,
April 15, 1999;
181(8):
2652 - 2654.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
H. E. Smith, M. Damman, J. van der Velde, F. Wagenaar, H. J. Wisselink, N. Stockhofe-Zurwieden, and M. A. Smits
Identification and Characterization of the cps Locus of Streptococcus suis Serotype 2: the Capsule Protects against Phagocytosis and Is an Important Virulence Factor
Infect. Immun.,
April 1, 1999;
67(4):
1750 - 1756.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. van Kranenburg, I. I. van Swam, J. D. Marugg, M. Kleerebezem, and W. M. de Vos
Exopolysaccharide Biosynthesis in Lactococcus lactis NIZO B40: Functional Analysis of the Glycosyltransferase Genes Involved in Synthesis of the Polysaccharide Backbone
J. Bacteriol.,
January 1, 1999;
181(1):
338 - 340.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
M. J. Cieslewicz, D. L. Kasper, Y. Wang, and M. R. Wessels
Functional Analysis in Type Ia Group B Streptococcus of a Cluster of Genes Involved in Extracellular Polysaccharide Production by Diverse Species of Streptococci
J. Biol. Chem.,
January 5, 2001;
276(1):
139 - 146.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|