HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pfister, M. F. Articles by Murer, H. Search for Related Content PubMed PubMed Citation Articles by Pfister, M. F. Articles by Murer, H. Social Bookmarking                      What's this?

Volume 272, Number 32, Issue of August 8, 1997 pp. 20125-20130
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Parathyroid Hormone-dependent Degradation of Type II Na⁺/P_i Cotransporters

(Received for publication, September 12, 1996, and in revised form, February 28, 1997)

Markus F. Pfister , Eleanor Lederer ^§ , Judith Forgo , Urs Ziegler ^¶ , Marius Lötscher , Elgar S. Quabius , Jürg Biber and Heini Murer

From the Institute of  Physiology and ^¶ Anatomy, University of Zurich, CH-8057 Zurich, Switzerland and the ^§ Department of Medicine, Division of Nephrology, University of Louisville, Louisville, Kentucky 40292

Parathyroid hormone (PTH) inhibits proximal tubular brush border membrane Na⁺/P_i cotransport activity; this decrease in the transport activity was found to be associated with a decrease in type II Na⁺/P_i cotransporter protein content in rat brush border membranes. In the present study we investigated the PTH-dependent regulation of the type II Na⁺/P_i cotransporter in opossum kidney cells, a previously established model to study cellular mechanisms involved in the regulation of proximal tubular Na⁺/P_i cotransport. We transfected opossum kidney cells with a cDNA coding for NaP_i-2 (rat renal type II Na⁺/P_i cotransporter). This allowed the study of PTH-dependent regulation of the transfected NaP_i-2 and of the corresponding intrinsic cotransporter (NaP_i-4). The results show (i) that the intrinsic and the transfected cotransporters are functionally (transport) and morphologically (immunofluorescence) localized at the apical membrane, (ii) that the intrinsic as well as the transfected Na⁺/P_i cotransport activities are inhibited by PTH, (iii) that PTH leads to a retrieval of both cotransporters from the apical membrane, (iv) that both cotransporters are rapidly degraded in response to PTH, and (v) that the reappearance/recovery of type II Na⁺/P_i cotransporter protein and function from PTH inhibition requires de novo protein synthesis. These results document that PTH leads to a removal of type II Na⁺/P_i cotransporters from the apical membrane and to their subsequent degradation.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. J. Mahon Ezrin promotes functional expression and parathyroid hormone-mediated regulation of the sodium-phosphate cotransporter 2a in LLC-PK1 cells Am J Physiol Renal Physiol, March 1, 2008; 294(3): F667 - F675. [Abstract] [Full Text] [PDF]

				P. Stratta, C. Canavese, M. Quaglia, E. Lazzarich, V. Morellini, M. Brustia, B. Bardone, and G. Bellomo A patient with unexplained hyperphosphataemia Nephrol. Dial. Transplant., September 1, 2006; 21(9): 2664 - 2666. [Full Text] [PDF]

				Z. Ristic, S. M. R. Camargo, E. Romeo, S. Bodoy, J. Bertran, M. Palacin, V. Makrides, E. M. Furrer, and F. Verrey Neutral amino acid transport mediated by ortholog of imino acid transporter SIT1/SLC6A20 in opossum kidney cells Am J Physiol Renal Physiol, April 1, 2006; 290(4): F880 - F887. [Abstract] [Full Text] [PDF]

				M. Klapper, H. Daniel, and F. Doring Cytosolic COOH terminus of the peptide transporter PEPT2 is involved in apical membrane localization of the protein Am J Physiol Cell Physiol, February 1, 2006; 290(2): C472 - C483. [Abstract] [Full Text] [PDF]

				K. J. Honegger, P. Capuano, C. Winter, D. Bacic, G. Stange, C. A. Wagner, J. Biber, H. Murer, and N. Hernando Regulation of sodium-proton exchanger isoform 3 (NHE3) by PKA and exchange protein directly activated by cAMP (EPAC) PNAS, January 17, 2006; 103(3): 803 - 808. [Abstract] [Full Text] [PDF]

				T. J. Berndt, S. Schiavi, and R. Kumar "Phosphatonins" and the regulation of phosphorus homeostasis Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1170 - F1182. [Abstract] [Full Text] [PDF]

				T. Berndt and R. Kumar The Phosphatonins and the Regulation of Phosphorus Homeostasis IBMS BoneKEy, June 1, 2005; 2(6): 5 - 16. [Full Text] [PDF]

				H. Xu, J. K. Uno, M. Inouye, J. F. Collins, and F. K. Ghishan NF1 transcriptional factor(s) is required for basal promoter activation of the human intestinal NaPi-IIb cotransporter gene Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G175 - G181. [Abstract] [Full Text] [PDF]

				M. J. Mahon and G. V. Segre Stimulation by Parathyroid Hormone of a NHERF-1-assembled Complex Consisting of the Parathyroid Hormone I Receptor, Phospholipase C{beta}, and Actin Increases Intracellular Calcium in Opossum Kidney Cells J. Biol. Chem., May 28, 2004; 279(22): 23550 - 23558. [Abstract] [Full Text] [PDF]

				H. S. Tenenhouse, C. Gauthier, H. Chau, and R. St.-Arnaud 1{alpha}-Hydroxylase gene ablation and Pi supplementation inhibit renal calcification in mice homozygous for the disrupted Npt2a gene Am J Physiol Renal Physiol, April 1, 2004; 286(4): F675 - F681. [Abstract] [Full Text] [PDF]

				W. Wang, C. Li, T.-H. Kwon, R. T. Miller, M. A. Knepper, J. Frokiaer, and S. Nielsen Reduced expression of renal Na+ transporters in rats with PTH-induced hypercalcemia Am J Physiol Renal Physiol, March 1, 2004; 286(3): F534 - F545. [Abstract] [Full Text]

				M. J. Mahon, J. A. Cole, E. D. Lederer, and G. V. Segre Na+/H+ Exchanger-Regulatory Factor 1 Mediates Inhibition of Phosphate Transport by Parathyroid Hormone and Second Messengers by Acting at Multiple Sites in Opossum Kidney Cells Mol. Endocrinol., November 1, 2003; 17(11): 2355 - 2364. [Abstract] [Full Text] [PDF]

				S. Pribanic, S. M. Gisler, D. Bacic, C. Madjdpour, N. Hernando, V. Sorribas, A. Gantenbein, J. Biber, and H. Murer Interactions of MAP17 with the NaPi-IIa/PDZK1 protein complex in renal proximal tubular cells Am J Physiol Renal Physiol, October 1, 2003; 285(4): F784 - F791. [Abstract] [Full Text] [PDF]

				Y. Moz, J. Silver, and T. Naveh-Many Characterization of cis-acting element in renal NaPi-2 cotransporter mRNA that determines mRNA stability Am J Physiol Renal Physiol, April 1, 2003; 284(4): F663 - F670. [Abstract] [Full Text] [PDF]

				S. J. Khundmiri, M. J. Rane, and E. D. Lederer Parathyroid Hormone Regulation of Type II Sodium-Phosphate Cotransporters Is Dependent on an A Kinase Anchoring Protein J. Biol. Chem., March 14, 2003; 278(12): 10134 - 10141. [Abstract] [Full Text] [PDF]

				N. Hernando, N. Deliot, S. M. Gisler, E. Lederer, E. J. Weinman, J. Biber, and H. Murer PDZ-domain interactions and apical expression of type IIa Na/Pi cotransporters PNAS, September 3, 2002; 99(18): 11957 - 11962. [Abstract] [Full Text] [PDF]

				M. E. Cavet, S. Akhter, R. Murtazina, F. Sanchez de Medina, C.-M. Tse, and M. Donowitz Half-lives of plasma membrane Na+/H+ exchangers NHE1-3: plasma membrane NHE2 has a rapid rate of degradation Am J Physiol Cell Physiol, December 1, 2001; 281(6): C2039 - C2048. [Abstract] [Full Text] [PDF]

				N. HERNANDO, J. FORGO, J. BIBER, and H. MURER PTH-Induced Downregulation of the Type IIa Na/Pi-Cotransporter Is Independent of Known Endocytic Motifs J. Am. Soc. Nephrol., November 1, 2000; 11(11): 1961 - 1968. [Abstract] [Full Text]

				Z. Karim-Jimenez, N. Hernando, J. Biber, and H. Murer A dibasic motif involved in parathyroid hormone-induced down-regulation of the type IIa NaPi cotransporter PNAS, October 23, 2000; (2000) 220394197. [Abstract] [Full Text]

				H. Murer, N. Hernando, I. Forster, and J. Biber Proximal Tubular Phosphate Reabsorption: Molecular Mechanisms Physiol Rev, October 1, 2000; 80(4): 1373 - 1409. [Abstract] [Full Text] [PDF]

				N. Zhao and H. S. Tenenhouse Npt2 Gene Disruption Confers Resistance to the Inhibitory Action of Parathyroid Hormone on Renal Sodium-Phosphate Cotransport Endocrinology, June 1, 2000; 141(6): 2159 - 2165. [Abstract] [Full Text] [PDF]

				M. Traebert, H. Volkl, J. Biber, H. Murer, and B. Kaissling Luminal and contraluminal action of 1-34 and 3-34 PTH peptides on renal type IIa Na-Pi cotransporter Am J Physiol Renal Physiol, May 1, 2000; 278(5): F792 - F798. [Abstract] [Full Text] [PDF]

				Z. Karim-Jimenez, N. Hernando, J. Biber, and H. Murer Requirement of a leucine residue for (apical) membrane expression of type IIb NaPi cotransporters PNAS, March 14, 2000; 97(6): 2916 - 2921. [Abstract] [Full Text] [PDF]

				N. Hernando, S. Sheikh, Z. Karim-Jimenez, H. Galliker, J. Forgo, J. Biber, and H. Murer Asymmetrical targeting of type II Na-Pi cotransporters in renal and intestinal epithelial cell lines Am J Physiol Renal Physiol, March 1, 2000; 278(3): F361 - F368. [Abstract] [Full Text] [PDF]

				E. D. LEDERER, S. S. SOHI, and K. R. MCLEISH Parathyroid Hormone Stimulates Extracellular Signal-Regulated Kinase (ERK) Activity Through Two Independent Signal Transduction Pathways: Roleof ERK in Sodium-Phosphate Cotransport J. Am. Soc. Nephrol., February 1, 2000; 11(2): 222 - 231. [Abstract] [Full Text] [PDF]

				M. Traebert, J. Roth, J. Biber, H. Murer, and B. Kaissling Internalization of proximal tubular type II Na-Pi cotransporter by PTH: immunogold electron microscopy Am J Physiol Renal Physiol, January 1, 2000; 278(1): F148 - F154. [Abstract] [Full Text] [PDF]

				Y. S. Guner, P. R. Kiela, H. Xu, J. F. Collins, and F. K. Ghishan Differential regulation of renal sodium-phosphate transporter by glucocorticoids during rat ontogeny Am J Physiol Cell Physiol, November 1, 1999; 277(5): C884 - C890. [Abstract] [Full Text] [PDF]

				H. Murer, I. Forster, N. Hernando, G. Lambert, M. Traebert, and J. Biber Posttranscriptional regulation of the proximal tubule NaPi-II transporter in response to PTH and dietary Pi Am J Physiol Renal Physiol, November 1, 1999; 277(5): F676 - F684. [Abstract] [Full Text] [PDF]

				M. TRAEBERT, M. LÖTSCHER, R. ASCHWANDEN, T. RITTHALER, J. BIBER, H. MURER, and B. KAISSLING Distribution of the Sodium/Phosphate Transporter during Postnatal Ontogeny of the Rat Kidney J. Am. Soc. Nephrol., July 1, 1999; 10(7): 1407 - 1415. [Abstract] [Full Text]

				I. C Forster, M. Traebert, M. Jankowski, G. Stange, J. Biber, and H. Murer Protein kinase C activators induce membrane retrieval of type II Na+-phosphate cotransporters expressed in Xenopus oocytes J. Physiol., June 1, 1999; 517(2): 327 - 340. [Abstract] [Full Text] [PDF]

				Y. Zhang, J. M. Norian, C. E. Magyar, N.-H. Holstein-Rathlou, A. K. Mircheff, and A. A. McDonough In vivo PTH provokes apical NHE3 and NaPi2 redistribution and Na-K-ATPase inhibition Am J Physiol Renal Physiol, May 1, 1999; 276(5): F711 - F719. [Abstract] [Full Text] [PDF]

				M. F. Pfister, J. Forgo, U. Ziegler, J. Biber, and H. Murer cAMP-dependent and -independent downregulation of type II Na-Pi cotransporters by PTH Am J Physiol Renal Physiol, May 1, 1999; 276(5): F720 - F725. [Abstract] [Full Text] [PDF]

				A. W. Jehle, H. Hilfiker, M. F. Pfister, J. Biber, E. Lederer, R. Krapf, and H. Murer Type II Na-Pi cotransport is regulated transcriptionally by ambient bicarbonate/carbon dioxide tension in OK cells Am J Physiol Renal Physiol, January 1, 1999; 276(1): F46 - F53. [Abstract] [Full Text] [PDF]

				E. D. Lederer, S. S. Sohi, J. M. Mathiesen, and J. B. Klein Regulation of expression of type II sodium-phosphate cotransporters by protein kinases A and C Am J Physiol Renal Physiol, August 1, 1998; 275(2): F270 - F277. [Abstract] [Full Text] [PDF]

				M. F. Pfister, I. Ruf, G. Stange, U. Ziegler, E. Lederer, J. Biber, and H. Murer Parathyroid hormone leads to the lysosomal degradation of the renal type II Na/Pi cotransporter PNAS, February 17, 1998; 95(4): 1909 - 1914. [Abstract] [Full Text] [PDF]

				H. Hilfiker, C. M. Hartmann, G. Stange, and H. Murer Characterization of the 5'-flanking region of OK cell type II Na-Pi cotransporter gene Am J Physiol Renal Physiol, January 1, 1998; 274(1): F197 - F204. [Abstract] [Full Text] [PDF]

				Z. Karim-Jimenez, N. Hernando, J. Biber, and H. Murer A dibasic motif involved in parathyroid hormone-induced down-regulation of the type IIa NaPi cotransporter PNAS, November 7, 2000; 97(23): 12896 - 12901. [Abstract] [Full Text] [PDF]

				W. Wang, T.-H. Kwon, C. Li, J. Frokiar, M. A. Knepper, and S. Nielsen Reduced expression of Na-K-2Cl cotransporter in medullary TAL in vitamin D-induced hypercalcemia in rats Am J Physiol Renal Physiol, January 1, 2002; 282(1): F34 - F44. [Abstract] [Full Text] [PDF]