HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Watanabe, M. Articles by Okada, N. Search for Related Content PubMed PubMed Citation Articles by Watanabe, M. Articles by Okada, N. Social Bookmarking                      What's this?

Volume 272, Number 32, Issue of August 8, 1997 pp. 20146-20151
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Biosynthesis of Archaeosine, a Novel Derivative of 7-Deazaguanosine Specific to Archaeal tRNA, Proceeds via a Pathway Involving Base Replacement on the tRNA Polynucleotide Chain

(Received for publication, April 7, 1997, and in revised form, May 28, 1997)

Masakatsu Watanabe , Mami Matsuo , Sonoko Tanaka , Hiroshi Akimoto ^¶ , Shuichi Asahi , Susumu Nishimura , Jon R. Katze ^** , Takeshi Hashizume , Pamela F. Crain , James A. McCloskey ^§§ and Norihiro Okada

From the  Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226, Japan, ^¶ Takeda Chemical Industries Ltd., Juso, Osaka 532, Japan,  Banyu Tsukuba Research Institute (Merck), Tsukuba 300-26, Japan, ^** Department of Microbiology and Immunology, University of Tennessee Memphis, Memphis, Tennessee 38163, and Departments of  Medicinal Chemistry and ^§§ Biochemistry, University of Utah, Salt Lake City, Utah 84112

Archaeosine is a novel derivative of 7-deazaguanosine found in transfer RNAs of most organisms exclusively in the archaeal phylogenetic lineage and is present in the D-loop at position 15. We show that this modification is formed by a posttranscriptional base replacement reaction, catalyzed by a new tRNA-guanine transglycosylase (TGT), which has been isolated from Haloferax volcanii and purified nearly to homogeneity. The molecular weight of the enzyme was estimated to be 78 kDa by SDS-gel electrophoresis. The enzyme can insert free 7-cyano-7-deazaguanine (preQ₀ base) in vitro at position 15 of an H. volcanii tRNA T7 transcript, replacing the guanine originally located at that position without breakage of the phosphodiester backbone. Since archaeosine base and 7-aminomethyl-7-deazaguanine (preQ₁ base) were not incorporated into tRNA by this enzyme, preQ₀ base appears to be the actual substrate for the TGT of H. volcanii, a conclusion supported by characterization of preQ₀ base in an acid-soluble extract of H. volcanii cells. Thus, this novel TGT in H. volcanii is a key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Phillips, B. El Yacoubi, B. Lyons, S. Alvarez, D. Iwata-Reuyl, and V. de Crecy-Lagard Biosynthesis of 7-Deazaguanosine-Modified tRNA Nucleosides: a New Role for GTP Cyclohydrolase I J. Bacteriol., December 15, 2008; 190(24): 7876 - 7884. [Abstract] [Full Text] [PDF]

				J. Sabina and D. Soll The RNA-binding PUA Domain of Archaeal tRNA-Guanine Transglycosylase Is Not Required for Archaeosine Formation J. Biol. Chem., March 17, 2006; 281(11): 6993 - 7001. [Abstract] [Full Text] [PDF]

				J. S. Reader, D. Metzgar, P. Schimmel, and V. de Crecy-Lagard Identification of Four Genes Necessary for Biosynthesis of the Modified Nucleoside Queuosine J. Biol. Chem., February 20, 2004; 279(8): 6280 - 6285. [Abstract] [Full Text] [PDF]

				J. D. Kittendorf, T. Sgraja, K. Reuter, G. Klebe, and G. A. Garcia An Essential Role for Aspartate 264 in Catalysis by tRNA-Guanine Transglycosylase from Escherichia coli J. Biol. Chem., October 24, 2003; 278(43): 42369 - 42376. [Abstract] [Full Text] [PDF]

				V. Anantharaman, E. V. Koonin, and L. Aravind Comparative genomics and evolution of proteins involved in RNA metabolism Nucleic Acids Res., April 1, 2002; 30(7): 1427 - 1464. [Abstract] [Full Text] [PDF]

				Y. Bai, D. T. Fox, J. A. Lacy, S. G. Van Lanen, and D. Iwata-Reuyl Hypermodification of tRNA in Thermophilic Archaea. CLONING, OVEREXPRESSION, AND CHARACTERIZATION OF tRNA-GUANINE TRANSGLYCOSYLASE FROM METHANOCOCCUS JANNASCHII J. Biol. Chem., September 8, 2000; 275(37): 28731 - 28738. [Abstract] [Full Text] [PDF]

				M. Watanabe, N. Nameki, M. Matsuo-Takasaki, S. Nishimura, and N. Okada tRNA Recognition of tRNA-guanine Transglycosylase from a Hyperthermophilic Archaeon, Pyrococcus horikoshii J. Biol. Chem., January 19, 2001; 276(4): 2387 - 2394. [Abstract] [Full Text] [PDF]