HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smith, M. D. Articles by Latchman, D. S. Search for Related Content PubMed PubMed Citation Articles by Smith, M. D. Articles by Latchman, D. S. Social Bookmarking                      What's this?

Volume 272, Number 34, Issue of August 22, 1997 pp. 21325-21333
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Coordinate Induction of the Three Neurofilament Genes by the Brn-3a Transcription Factor

(Received for publication, December 30, 1996, and in revised form, March 4, 1997)

Martin D. Smith , Peter J. Morris , Sally J. Dawson , Michael L. Schwartz ^§ , William W. Schlaepfer ^§ and David S. Latchman

From the  Department of Molecular Pathology, University College London Medical School, London W1P 6DB, United Kingdom and the ^§ Division of Neuropathology, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19104

The POU domain transcription factor Brn-3a is able to stimulate neurite outgrowth when overexpressed in the neuronal ND7 cell line, whereas the closely related Brn-3b factor does not have this effect. We show that Brn-3a overexpression also enhances the expression of the three neurofilament genes at both the mRNA and protein levels, whereas Brn-3b overexpression has no effect. In addition Brn-3a activates the three neurofilament gene promoters in co-transfection assays in both neuronal and non-neuronal cells. As observed for enhanced neurite outgrowth, the stimulation of neurofilament gene expression and activation of the neurofilament gene promoters is observed with the isolated POU domain of Brn-3a. A single amino acid change in the POU homeodomain of Brn-3a to the equivalent amino acid in Brn-3b abolishes its ability to activate the neurofilament promoters, whereas the reciprocal change converts Brn-3b to an activator of these promoters.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				V. S. Budhram-Mahadeo, S. Bowen, S. Lee, C. Perez-Sanchez, E. Ensor, P. J. Morris, and D. S. Latchman Brn-3b enhances the pro-apoptotic effects of p53 but not its induction of cell cycle arrest by cooperating in trans-activation of bax expression Nucleic Acids Res., December 2, 2006; 34(22): 6640 - 6652. [Abstract] [Full Text] [PDF]

				C. D. Hudson, P. J. Morris, D. S. Latchman, and V. S. Budhram-Mahadeo Brn-3a Transcription Factor Blocks p53-mediated Activation of Proapoptotic Target Genes Noxa and Bax in Vitro and in Vivo to Determine Cell Fate J. Biol. Chem., March 25, 2005; 280(12): 11851 - 11858. [Abstract] [Full Text] [PDF]

				S. R. Eng, J. Lanier, N. Fedtsova, and E. E. Turner Coordinated regulation of gene expression by Brn3a in developing sensory ganglia Development, August 15, 2004; 131(16): 3859 - 3870. [Abstract] [Full Text] [PDF]

				S. Irshad, R. B. Pedley, J. Anderson, D. S. Latchman, and V. Budhram-Mahadeo The Brn-3b Transcription Factor Regulates the Growth, Behavior, and Invasiveness of Human Neuroblastoma Cells in Vitro and in Vivo J. Biol. Chem., May 14, 2004; 279(20): 21617 - 21627. [Abstract] [Full Text] [PDF]

				L. Ma, L. Lei, S. R. Eng, E. Turner, and L. F. Parada Brn3a regulation of TrkA/NGF receptor expression in developing sensory neurons Development, August 1, 2003; 130(15): 3525 - 3534. [Abstract] [Full Text] [PDF]

				J. Y. Sze, S. Zhang, J. Li, and G. Ruvkun The C. elegans POU-domain transcription factor UNC-86 regulates the tph-1 tryptophan hydroxylase gene and neurite outgrowth in specific serotonergic neurons Development, March 10, 2003; 129(16): 3901 - 3911. [Abstract] [Full Text] [PDF]

				C. Perez-Sanchez, V. S. Budhram-Mahadeo, and D. S. Latchman Distinct promoter elements mediate the co-operative effect of Brn-3a and p53 on the p21 promoter and their antagonism on the Bax promoter Nucleic Acids Res., November 15, 2002; 30(22): 4872 - 4880. [Abstract] [Full Text] [PDF]

				K. L. Sugars, V. Budhram-Mahadeo, G. Packham, and D. S. Latchman A minimal Bcl-x promoter is activated by Brn-3a and repressed by p53 Nucleic Acids Res., November 15, 2001; 29(22): 4530 - 4540. [Abstract] [Full Text] [PDF]

				F. K. Bedford, D. Julius, and H. A. Ingraham Neuronal Expression of the 5HT3 Serotonin Receptor Gene Requires Nuclear Factor 1 Complexes J. Neurosci., August 15, 1998; 18(16): 6186 - 6194. [Abstract] [Full Text] [PDF]

				E. Ensor, M. D. Smith, and D. S. Latchman The BRN-3A Transcription Factor Protects Sensory but Not Sympathetic Neurons from Programmed Cell Death/Apoptosis J. Biol. Chem., February 9, 2001; 276(7): 5204 - 5212. [Abstract] [Full Text] [PDF]