|
|
|
|||||||
|
|||||||||
(Received for publication, April 9, 1997)
From the Desmosomes are cell junctions that
act as sites of strong intercellular adhesion and also serve to anchor
the intermediate filament (IF) cytoskeleton to the plasma membrane of a
variety of cell types. Previous studies demonstrated that the COOH
terminus of the desmosomal plaque protein, desmoplakin (DP), is
required for the association of DP with IF networks in cultured cells
and that this domain interacts directly with type II epidermal keratin polypeptides in vitro. However, these studies left open the
question of how desmosomes might anchor other IF types known to
associate with these junctions. In this report we used yeast two-hybrid and in vitro dot blot assays to further examine the
requirements for direct interactions between desmoplakin and various IF
types.
Our results confirm the ability of the DP COOH terminus (DPCT) to
interact with at least two regions of the head domain of the type II
epidermal keratin K1 and also demonstrate that DPCT can interact with
the type III IF family members, vimentin and desmin, as well as simple
epithelial keratins. Unlike the situation for type II epidermal
keratins, the interaction between DPCT and simple epithelial keratins
appears to depend on heterodimerization of the type I and II keratin
polypeptides, since both are required to detect an interaction.
Furthermore, although the interaction between DPCT and K1 requires the
keratin head domain, deletion of this domain from the simple epithelial
keratins does not compromise interaction with DPCT. The interaction
between DPCT and type III or simple epithelial keratins also appeared
to be less robust than that between DPCT and K1. In the case of K8/K18,
however, the interaction as assessed by yeast two-hybrid assays
increased 9-fold when a serine located in a protein kinase A consensus
phosphorylation site 23 residues from the end of DP was altered to a
glycine. Taken together, these data indicate that DP interacts directly with different IF types in specific ways.
Volume 272, Number 34,
Issue of August 22, 1997
pp. 21495-21503
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
,
Department of Cell Biology, Neurobiology and
Anatomy, University of Cincinnati College of Medicine, Cincinnati, Ohio
45267-0521, the § Departments of Pathology and Dermatology,
Northwestern University Medical School, Chicago, Illinois 60611, and the ¶ Laboratory of Skin Biology, NIAMS, National Institutes
of Health, Bethesda, Maryland 20892
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. E. Bass-Zubek, R. P. Hobbs, E. V. Amargo, N. J. Garcia, S. N. Hsieh, X. Chen, J. K. Wahl III, M. F. Denning, and K. J. Green Plakophilin 2: a critical scaffold for PKC{alpha} that regulates intercellular junction assembly J. Cell Biol., October 17, 2008; 181(4): 605 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Spazierer, J. Raberger, K. Gross, P. Fuchs, and G. Wiche Stress-induced recruitment of epiplakin to keratin networks increases their resistance to hyperphosphorylation-induced disruption J. Cell Sci., March 15, 2008; 121(6): 825 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lapouge, L. Fontao, M.-F. Champliaud, F. Jaunin, M. A. Frias, B. Favre, D. Paulin, K. J. Green, and L. Borradori New insights into the molecular basis of desmoplakinand desmin-related cardiomyopathies J. Cell Sci., December 1, 2006; 119(23): 4974 - 4985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Kottke, E. Delva, and A. P. Kowalczyk The desmosome: cell science lessons from human diseases. J. Cell Sci., March 1, 2006; 119(Pt 5): 797 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Dusek, S. Getsios, F. Chen, J. K. Park, E. V. Amargo, V. L. Cryns, and K. J. Green The Differentiation-dependent Desmosomal Cadherin Desmoglein 1 Is a Novel Caspase-3 Target That Regulates Apoptosis in Keratinocytes J. Biol. Chem., February 10, 2006; 281(6): 3614 - 3624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Goto, H. Sumiyoshi, T. Sakai, R. Fassler, S. Ohashi, E. Adachi, H. Yoshioka, and S. Fujiwara Elimination of Epiplakin by Gene Targeting Results in Acceleration of Keratinocyte Migration in Mice Mol. Cell. Biol., January 15, 2006; 26(2): 548 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Godsel, S. N. Hsieh, E. V. Amargo, A. E. Bass, L. T. Pascoe-McGillicuddy, A. C. Huen, M. E. Thorne, C. A. Gaudry, J. K. Park, K. Myung, et al. Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin J. Cell Biol., December 19, 2005; 171(6): 1045 - 1059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-I. Jang, A. Kalinin, K. Takahashi, L. N. Marekov, and P. M. Steinert Characterization of human epiplakin: RNAi-mediated epiplakin depletion leads to the disruption of keratin and vimentin IF networks J. Cell Sci., February 15, 2005; 118(4): 781 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Owens, N. J. Wilson, A. J. M. Hill, E. L. Rugg, R. M. Porter, A. M. Hutcheson, R. A. Quinlan, D. van Heel, M. Parkes, D. P. Jewell, et al. Human keratin 8 mutations that disturb filament assembly observed in inflammatory bowel disease patients J. Cell Sci., April 15, 2004; 117(10): 1989 - 1999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Spazierer, P. Fuchs, V. Proll, L. Janda, S. Oehler, I. Fischer, R. Hauptmann, and G. Wiche Epiplakin Gene Analysis in Mouse Reveals a Single Exon Encoding a 725-kDa Protein with Expression Restricted to Epithelial Tissues J. Biol. Chem., August 22, 2003; 278(34): 31657 - 31666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fontao, B. Favre, S. Riou, D. Geerts, F. Jaunin, J.-H. Saurat, K. J. Green, A. Sonnenberg, and L. Borradori Interaction of the Bullous Pemphigoid Antigen 1 (BP230) and Desmoplakin with Intermediate Filaments Is Mediated by Distinct Sequences within Their COOH Terminus Mol. Biol. Cell, May 1, 2003; 14(5): 1978 - 1992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Karashima and F. M. Watt Interaction of periplakin and envoplakin with intermediate filaments J. Cell Sci., March 14, 2003; 115(24): 5027 - 5037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Koster, D. Geerts, B. Favre, L. Borradori, and A. Sonnenberg Analysis of the interactions between BP180, BP230, plectin and the integrin {alpha}6{beta}4 important for hemidesmosome assembly J. Cell Sci., January 15, 2003; 116(2): 387 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Calkins, B. L. Hoepner, C. M. Law, M. R. Novak, S. V. Setzer, M. Hatzfeld, and A. P. Kowalczyk The Armadillo Family Protein p0071 Is a VE-cadherin- and Desmoplakin-binding Protein J. Biol. Chem., January 10, 2003; 278(3): 1774 - 1783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Huen, J. K. Park, L. M. Godsel, X. Chen, L. J. Bannon, E. V. Amargo, T. Y. Hudson, A. K. Mongiu, I. M. Leigh, D. P. Kelsell, et al. Intermediate filament-membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength J. Cell Biol., December 23, 2002; 159(6): 1005 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Beil, J. Leser, M. P. Lutz, A. Gukovskaya, T. Seufferlein, G. Lynch, S. J. Pandol, and G. Adler Caspase 8-mediated cleavage of plectin precedes F-actin breakdown in acinar cells during pancreatitis Am J Physiol Gastrointest Liver Physiol, March 1, 2002; 282(3): G450 - G460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reichelt, H. Bussow, C. Grund, and T. M. Magin Formation of a Normal Epidermis Supported by Increased Stability of Keratins 5 and 14 in Keratin 10 Null Mice Mol. Biol. Cell, June 1, 2001; 12(6): 1557 - 1568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Peters, J. Kirfel, H. Bussow, M. Vidal, and T. M. Magin Complete Cytolysis and Neonatal Lethality in Keratin 5 Knockout Mice Reveal Its Fundamental Role in Skin Integrity and in Epidermolysis Bullosa Simplex Mol. Biol. Cell, June 1, 2001; 12(6): 1775 - 1789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bornslaeger, L. Godsel, C. Corcoran, J. Park, M Hatzfeld, A. Kowalczyk, and K. Green Plakophilin 1 interferes with plakoglobin binding to desmoplakin, yet together with plakoglobin promotes clustering of desmosomal plaque complexes at cell-cell borders J. Cell Sci., January 2, 2001; 114(4): 727 - 738. [Abstract] [PDF]
|
|
|
|
|
|
DiColandrea, Karashima, Maata, and F. Watt Subcellular Distribution of Envoplakin and Periplakin: Insights into Their Role as Precursors of the Epidermal Cornified Envelope J. Cell Biol., October 30, 2000; 151(3): 573 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shi and S. P. Sugrue Dissection of Protein Linkage between Keratins and Pinin, a Protein with Dual Location at Desmosome-Intermediate Filament Complex and in the Nucleus J. Biol. Chem., May 12, 2000; 275(20): 14910 - 14915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hatzfeld, C. Haffner, K. Schulze, and U. Vinzens The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization J. Cell Biol., April 3, 2000; 149(1): 209 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Magin, H. Kaiser, S Leitgeb, C Grund, I. Leigh, S. Morley, and E. Lane Supplementation of a mutant keratin by stable expression of desmin in cultured human EBS keratinocytes J. Cell Sci., January 12, 2000; 113(23): 4231 - 4239. [Abstract] [PDF]
|
|
|
|
|
|
I Hofmann, C Mertens, M Brettel, V Nimmrich, M Schnolzer, and H Herrmann Interaction of plakophilins with desmoplakin and intermediate filament proteins: an in vitro analysis J. Cell Sci., January 7, 2000; 113(13): 2471 - 2483. [Abstract] [PDF]
|
|
|
|
 |
|
 R. B. Presland and B. A. Dale Epithelial Structural Proteins of the Skin and Oral Cavity: Function in Health and Disease Critical Reviews in Oral Biology & Medicine, January 1, 2000; 11(4): 383 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Geerts, L. Fontao, M. G. Nievers, R. Q.J. Schaapveld, P. E. Purkis, G. N. Wheeler, E. B. Lane, I. M. Leigh, and A. Sonnenberg Binding of Integrin {alpha}6{beta}4 to Plectin Prevents Plectin Association with F-Actin but Does Not Interfere with Intermediate Filament Binding J. Cell Biol., October 18, 1999; 147(2): 417 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Kowalczyk, M. Hatzfeld, E. A. Bornslaeger, D. S. Kopp, J. E. Borgwardt, C. M. Corcoran, A. Settler, and K. J. Green The Head Domain of Plakophilin-1 Binds to Desmoplakin and Enhances Its Recruitment to Desmosomes. IMPLICATIONS FOR CUTANEOUS DISEASE J. Biol. Chem., June 25, 1999; 274(26): 18145 - 18148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Steinert, Y.-H. Chou, V. Prahlad, D. A. D. Parry, L. N. Marekov, K. C. Wu, S.-I. Jang, and R. D. Goldman A High Molecular Weight Intermediate Filament-associated Protein in BHK-21 Cells Is Nestin, a Type VI Intermediate Filament Protein. LIMITED CO-ASSEMBLY IN VITRO TO FORM HETEROPOLYMERS WITH TYPE III VIMENTIN AND TYPE IV alpha -INTERNEXIN J. Biol. Chem., April 2, 1999; 274(14): 9881 - 9890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Leung, D. Sun, and R. K.H. Liem The Intermediate Filament Protein Peripherin Is the Specific Interaction Partner of Mouse BPAG1-n (Dystonin) in Neurons J. Cell Biol., February 8, 1999; 144(3): 435 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. North, W. Bardsley, J Hyam, E. Bornslaeger, H. Cordingley, B Trinnaman, M Hatzfeld, K. Green, A. Magee, and D. Garrod Molecular map of the desmosomal plaque J. Cell Sci., January 12, 1999; 112(23): 4325 - 4336. [Abstract] [PDF]
|
|
|
|
|
|
R. D. Paladini and P. A. Coulombe Directed Expression of Keratin 16 to the Progenitor Basal Cells of Transgenic Mouse Skin Delays Skin Maturation J. Cell Biol., August 24, 1998; 142(4): 1035 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Smith and E. Fuchs Defining the Interactions Between Intermediate Filaments and Desmosomes J. Cell Biol., June 1, 1998; 141(5): 1229 - 1241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Candi, E. Tarcsa, J. J. Digiovanna, J. G. Compton, P. M. Elias, L. N. Marekov, and P. M. Steinert A highly conserved lysine residue on the head domain of type II keratins is essential for the attachment of keratin intermediate filaments to the cornified cell envelope through isopeptide crosslinking by transglutaminases PNAS, March 3, 1998; 95(5): 2067 - 2072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kowalczyk, P Navarro, E Dejana, E. Bornslaeger, K. Green, D. Kopp, and J. Borgwardt VE-cadherin and desmoplakin are assembled into dermal microvascular endothelial intercellular junctions: a pivotal role for plakoglobin in the recruitment of desmoplakin to intercellular junctions J. Cell Sci., January 10, 1998; 111(20): 3045 - 3057. [Abstract] [PDF]
|
|
|
|
|
|
S. Norvell and K. Green Contributions of extracellular and intracellular domains of full length and chimeric cadherin molecules to junction assembly in epithelial cells J. Cell Sci., January 5, 1998; 111(9): 1305 - 1318. [Abstract] [PDF]
|
|
|
|
|
|
A. P. Kowalczyk, E. A. Bornslaeger, J. E. Borgwardt, H. L. Palka, A. S. Dhaliwal, C. M. Corcoran, M. F. Denning, and K. J. Green The Amino-terminal Domain of Desmoplakin Binds to Plakoglobin and Clusters Desmosomal Cadherin-Plakoglobin Complexes J. Cell Biol., November 3, 1997; 139(3): 773 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ilan, L. Cheung, E. Pinter, and J. A. Madri Platelet-Endothelial Cell Adhesion Molecule-1 (CD31), a Scaffolding Molecule for Selected Catenin Family Members Whose Binding Is Mediated by Different Tyrosine and Serine/Threonine Phosphorylation J. Biol. Chem., July 7, 2000; 275(28): 21435 - 21443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Bellin, T. W. Huiatt, D. R. Critchley, and R. M. Robson Synemin May Function to Directly Link Muscle Cell Intermediate Filaments to Both Myofibrillar Z-lines and Costameres J. Biol. Chem., August 17, 2001; 276(34): 32330 - 32337. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |