| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, June 2, 1997, and in revised form, June 24, 1997)
From the
Volume 272, Number 34,
Issue of August 22, 1997
pp. 21504-21508
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
-Latrotoxin Receptor, Latrophilin, Is a Novel Member of the
Secretin Family of G Protein-coupled Receptors
,
,
,
Department of Biochemistry,
-Latrotoxin (LTX) stimulates massive
exocytosis of synaptic vesicles and may help to elucidate the mechanism
of regulation of neurosecretion. We have recently isolated latrophilin,
the synaptic Ca2+-independent LTX receptor. Now we
demonstrate that latrophilin is a novel member of the secretin family
of G protein-coupled receptors that are involved in secretion. Northern
blot analysis shows that latrophilin message is present only in
neuronal tissue. Upon expression in COS cells, the cloned protein is
indistinguishable from brain latrophilin and binds LTX with high
affinity. Latrophilin physically interacts with a Go
subunit of heterotrimeric G proteins, because the two proteins
co-purify in a two-step affinity chromatography. Interestingly,
extracellular domain of latrophilin is homologous to olfactomedin, a
soluble neuronal protein thought to participate in odorant binding. Our
findings suggest that latrophilin may bind unidentified endogenous
ligands and transduce signals into nerve terminals, thus implicating G
proteins in the control of synaptic vesicle exocytosis.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Q. Davies, G.-W. Chang, S. Yona, S. Gordon, M. Stacey, and H.-H. Lin The Role of Receptor Oligomerization in Modulating the Expression and Function of Leukocyte Adhesion-G Protein-coupled Receptors J. Biol. Chem., September 14, 2007; 282(37): 27343 - 27353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Jin, I. Tietjen, L. Bu, L. Liu-Yesucevitz, S. K. Gaur, C. A. Walsh, and X. Piao Disease-associated mutations affect GPR56 protein trafficking and cell surface expression Hum. Mol. Genet., August 15, 2007; 16(16): 1972 - 1985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lajus, P. Vacher, D. Huber, M. Dubois, M.-N. Benassy, Y. Ushkaryov, and J. Lang {alpha}-Latrotoxin Induces Exocytosis by Inhibition of Voltage-dependent K+ Channels and by Stimulation of L-type Ca2+ Channels via Latrophilin in beta-Cells J. Biol. Chem., March 3, 2006; 281(9): 5522 - 5531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li, D. Lee, L. Wang, M. Khvotchev, S. K. Chiew, L. Arunachalam, T. Collins, Z.-P. Feng, and S. Sugita N-Terminal Insertion and C-Terminal Ankyrin-Like Repeats of {alpha}-Latrotoxin Are Critical for Ca2+-Dependent Exocytosis J. Neurosci., November 2, 2005; 25(44): 10188 - 10197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Grinchuk, Z. Kozmik, X. Wu, and S. Tomarev The Optimedin Gene Is a Downstream Target of Pax6 J. Biol. Chem., October 21, 2005; 280(42): 35228 - 35237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Hardy, E. A. Hoffman, P. Gonzalez, B. S. McKay, and W. D. Stamer Extracellular Trafficking of Myocilin in Human Trabecular Meshwork Cells J. Biol. Chem., August 12, 2005; 280(32): 28917 - 28926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ahmed, M. Torrado, R. D. Zinovieva, V. V. Senatorov, G. Wistow, and S. I. Tomarev Gene Expression Profile of the Rat Eye Iridocorneal Angle: NEIBank Expressed Sequence Tag Analysis Invest. Ophthalmol. Vis. Sci., September 1, 2004; 45(9): 3081 - 3090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Loria, J. Hodgkin, and O. Hobert A Conserved Postsynaptic Transmembrane Protein Affecting Neuromuscular Signaling in Caenorhabditis elegans J. Neurosci., March 3, 2004; 24(9): 2191 - 2201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Volynski, M. Capogna, A. C. Ashton, D. Thomson, E. V. Orlova, C. F. Manser, R. R. Ribchester, and Y. A. Ushkaryov Mutant {alpha}-Latrotoxin (LTXN4C) Does Not Form Pores and Causes Secretion by Receptor Stimulation: THIS ACTION DOES NOT REQUIRE NEUREXINS J. Biol. Chem., August 15, 2003; 278(33): 31058 - 31066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Capogna, K. E. Volynski, N. J. Emptage, and Y. A. Ushkaryov The {alpha}-Latrotoxin Mutant LTXN4C Enhances Spontaneous and Evoked Transmitter Release in CA3 Pyramidal Neurons J. Neurosci., May 15, 2003; 23(10): 4044 - 4053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Krasnoperov, Y. Lu, L. Buryanovsky, T. A. Neubert, K. Ichtchenko, and A. G. Petrenko Post-translational Proteolytic Processing of the Calcium-independent Receptor of alpha -Latrotoxin (CIRL), a Natural Chimera of the Cell Adhesion Protein and the G Protein-coupled Receptor. ROLE OF THE G PROTEIN-COUPLED RECEPTOR PROTEOLYSIS SITE (GPS) MOTIF J. Biol. Chem., November 22, 2002; 277(48): 46518 - 46526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ouanounou, M. Malo, J. Stinnakre, A. S. Kreger, and J. Molgo Trachynilysin, a Neurosecretory Protein Isolated from Stonefish (Synanceia trachynis) Venom, Forms Nonselective Pores in the Membrane of NG108-15 Cells J. Biol. Chem., October 11, 2002; 277(42): 39119 - 39127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Krasnoperov, M. A. Bittner, W. Mo, L. Buryanovsky, T. A. Neubert, R. W. Holz, K. Ichtchenko, and A. G. Petrenko Protein-tyrosine Phosphatase-sigma Is a Novel Member of the Functional Family of alpha -Latrotoxin Receptors J. Biol. Chem., September 20, 2002; 277(39): 35887 - 35895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Stacey, G.-W. Chang, S. L. Sanos, L. R. Chittenden, L. Stubbs, S. Gordon, and H.-H. Lin EMR4, a Novel Epidermal Growth Factor (EGF)-TM7 Molecule Up-regulated in Activated Mouse Macrophages, Binds to a Putative Cellular Ligand on B Lymphoma Cell Line A20 J. Biol. Chem., August 2, 2002; 277(32): 29283 - 29293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Abe, T. Fukuzawa, and S. Hirose Cleavage of Ig-Hepta at a "SEA" Module and at a Conserved G Protein-coupled Receptor Proteolytic Site J. Biol. Chem., June 21, 2002; 277(26): 23391 - 23398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tobaben, T. C. Sudhof, and B. Stahl Genetic Analysis of alpha -Latrotoxin Receptors Reveals Functional Interdependence of CIRL/Latrophilin 1 and Neurexin 1alpha J. Biol. Chem., February 15, 2002; 277(8): 6359 - 6365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Terskikh, M. C. Easterday, L. Li, L. Hood, H. I. Kornblum, D. H. Geschwind, and I. L. Weissman From the Cover: From hematopoiesis to neuropoiesis: Evidence of overlapping genetic programs PNAS, July 3, 2001; 98(14): 7934 - 7939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Tsang, D. B. Elrick, and M. P. Charlton alpha -Latrotoxin Releases Calcium in Frog Motor Nerve Terminals J. Neurosci., December 1, 2000; 20(23): 8685 - 8692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nikkila, D. R. McMillan, B. S. Nunez, L. Pascoe, K. M. Curnow, and P. C. White Sequence Similarities between a Novel Putative G Protein-Coupled Receptor and Na+/Ca2+ Exchangers Define a Cation Binding Domain Mol. Endocrinol., September 1, 2000; 14(9): 1351 - 1364. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Hamann, C. van Zeventer, A. Bijl, C. Molenaar, K. Tesselaar, and R. A. W. van Lier Molecular cloning and characterization of mouse CD97 Int. Immunol., April 1, 2000; 12(4): 439 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Schiavo, M. Matteoli, and C. Montecucco Neurotoxins Affecting Neuroexocytosis Physiol Rev, April 1, 2000; 80(2): 717 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Hlubek, E. L. Stuenkel, V. G. Krasnoperov, A. G. Petrenko, and R. W. Holz Calcium-Independent Receptor for alpha -Latrotoxin and Neurexin H Facilitate Toxin-Induced Channel Formation: Evidence That Channel Formation Results from Tethering of Toxin to Membrane Mol. Pharmacol., March 1, 2000; 57(3): 519 - 528. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. B. Elrick and M. P. Charlton alpha -Latrocrustatoxin Increases Neurotransmitter Release by Activating a Calcium Influx Pathway at Crayfish Neuromuscular Junction J Neurophysiol, December 1, 1999; 82(6): 3550 - 3562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Abe, H. Suzuki, M. Notoya, T. Yamamoto, and S. Hirose Ig-Hepta, a Novel Member of the G Protein-coupled Hepta-helical Receptor (GPCR) Family That Has Immunoglobulin-like Repeats in a Long N-terminal Extracellular Domain and Defines a New Subfamily of GPCRs J. Biol. Chem., July 9, 1999; 274(28): 19957 - 19964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. W. Tse and A. Tse {alpha}-Latrotoxin Stimulates Inward Current, Rise in Cytosolic Calcium Concentration, and Exocytosis in at Pituitary Gonadotropes Endocrinology, July 1, 1999; 140(7): 3025 - 3033. [Abstract] [Full Text]
|
|
|
|
|
|
K. Ichtchenko, M. A. Bittner, V. Krasnoperov, A. R. Little, O. Chepurny, R. W. Holz, and A. G. Petrenko A Novel Ubiquitously Expressed alpha -Latrotoxin Receptor Is a Member of the CIRL Family of G-protein-coupled Receptors J. Biol. Chem., February 26, 1999; 274(9): 5491 - 5498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Krasnoperov, M. A. Bittner, R. W. Holz, O. Chepurny, and A. G. Petrenko Structural Requirements for alpha -Latrotoxin Binding and alpha -Latrotoxin-stimulated Secretion. A STUDY WITH CALCIUM-INDEPENDENT RECEPTOR OF alpha -LATROTOXIN (CIRL) DELETION MUTANTS J. Biol. Chem., February 5, 1999; 274(6): 3590 - 3596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Piétu, R. Mariage-Samson, N.-A. Fayein, C. Matingou, E. Eveno, R. Houlgatte, C. Decraene, Y. Vandenbrouck, F. Tahi, M.-D. Devignes, et al. The Genexpress IMAGE Knowledge Base of the Human Brain Transcriptome: A Prototype Integrated Resource for Functional and Computational Genomics Genome Res., February 1, 1999; 9(2): 195 - 209. [Abstract] [Full Text]
|
|
|
|
|
|
M. Missler, R. E. Hammer, and T. C. Sudhof Neurexophilin Binding to alpha -Neurexins. A SINGLE LNS DOMAIN FUNCTIONS AS AN INDEPENDENTLY FOLDING LIGAND-BINDING UNIT J. Biol. Chem., December 25, 1998; 273(52): 34716 - 34723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sugita, K. Ichtchenko, M. Khvotchev, and T. C. Sudhof alpha -Latrotoxin Receptor CIRL/Latrophilin 1 (CL1) Defines an Unusual Family of Ubiquitous G-protein-linked Receptors. G-PROTEIN COUPLING NOT REQUIRED FOR TRIGGERING EXOCYTOSIS J. Biol. Chem., December 4, 1998; 273(49): 32715 - 32724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Thomas and L. A. Elferink Functional Analysis of the C2A Domain of Synaptotagmin 1: Implications for Calcium-Regulated Secretion J. Neurosci., May 15, 1998; 18(10): 3511 - 3520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Bittner, V. G. Krasnoperov, E. L. Stuenkel, A. G. Petrenko, and R. W. Holz A Ca2+-Independent Receptor for alpha -Latrotoxin, CIRL, Mediates Effects on Secretion via Multiple Mechanisms J. Neurosci., April 15, 1998; 18(8): 2914 - 2922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Geppert, M. Khvotchev, V. Krasnoperov, Y. Goda, M. Missler, R. E. Hammer, K. Ichtchenko, A. G. Petrenko, and T. C. Sudhof Neurexin Ialpha Is a Major alpha -Latrotoxin Receptor That Cooperates in alpha -Latrotoxin Action J. Biol. Chem., January 16, 1998; 273(3): 1705 - 1710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sugita, F. Saito, J. Tang, J. Satz, K. Campbell, and T. C. Sudhof A stoichiometric complex of neurexins and dystroglycan in brain J. Cell Biol., July 23, 2001; 154(2): 435 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Bittner and R. W. Holz Latrotoxin Stimulates Secretion in Permeabilized Cells by Regulating an Intracellular Ca2+- and ATP-dependent Event. A ROLE FOR PROTEIN KINASE C J. Biol. Chem., August 11, 2000; 275(33): 25351 - 25357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Volynski, F. A. Meunier, V. G. Lelianova, E. E. Dudina, T. M. Volkova, M. A. Rahman, C. Manser, E. V. Grishin, J. O. Dolly, R. H. Ashley, et al. Latrophilin, Neurexin, and Their Signaling-deficient Mutants Facilitate alpha -Latrotoxin Insertion into Membranes but Are Not Involved in Pore Formation J. Biol. Chem., December 22, 2000; 275(52): 41175 - 41183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Stacey, H.-H. Lin, K. L. Hilyard, S. Gordon, and A. J. McKnight Human Epidermal Growth Factor (EGF) Module-containing Mucin-like Hormone Receptor 3 Is a New Member of the EGF-TM7 Family That Recognizes a Ligand on Human Macrophages and Activated Neutrophils J. Biol. Chem., May 25, 2001; 276(22): 18863 - 18870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Ashton, K. E. Volynski, V. G. Lelianova, E. V. Orlova, C. Van Renterghem, M. Canepari, M. Seagar, and Y. A. Ushkaryov alpha -Latrotoxin, Acting via Two Ca2+-dependent Pathways, Triggers Exocytosis of Two Pools of Synaptic Vesicles J. Biol. Chem., November 21, 2001; 276(48): 44695 - 44703. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
