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Volume 272, Number 35, Issue of August 29, 1997 pp. 21927-21931
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Xer Recombination in Escherichia coli
SITE-SPECIFIC DNA TOPOISOMERASE ACTIVITY OF THE XerC and XerD RECOMBINASES

(Received for publication, April 30, 1997, and in revised form, June 25, 1997)

François Cornet , Bernard Hallet and David J. Sherratt

From the Microbiology Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom

Xer site-specific recombination functions in maintaining circular replicons in the monomeric state in Escherichia coli. Two recombinases of the bacteriophage lambda  integrase family, XerC and XerD, are required for recombination at the chromosomal site, dif, and at a range of plasmid-borne sites. Xer recombination core sites contain the 11-base pair binding sites for each recombinase separated by a 6 to 8-base pair central region. We report that both XerC and XerD act as site-specific type I topoisomerases by relaxing supercoiled plasmids containing a dif site. Relaxation by either XerC or XerD occurs in the absence of the partner recombinase and requires only a single recombination core site. XerC or XerD relaxation activities are completely inhibited by the addition of the partner recombinase, providing that the DNA recognition sequence for the inhibiting partner is present.


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Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.