HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sudbeck, B. D. Articles by Parks, W. C. Search for Related Content PubMed PubMed Citation Articles by Sudbeck, B. D. Articles by Parks, W. C. Social Bookmarking                      What's this?

Volume 272, Number 35, Issue of August 29, 1997 pp. 22103-22110
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Induction and Repression of Collagenase-1 by Keratinocytes Is Controlled by Distinct Components of Different Extracellular Matrix Compartments

(Received for publication, April 18, 1997, and in revised form, June 5, 1997)

Barry D. Sudbeck , Brian K. Pilcher , Howard G. Welgus and William C. Parks ^¶

From the Departments of  Medicine (Dermatology) and ^¶ Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110

In all forms of cutaneous wounds, collagenase-1 (matrix metalloproteinase-1 (MMP-1)) is invariably expressed by basal keratinocytes migrating over the dermal matrix. We report that native type I collagen mediates induction of MMP-1 by primary human keratinocytes. Collagen-mediated induction of MMP-1 was rapid, being detected 2 h after plating, and was transcriptionally regulated. As demonstrated by in situ hybridization, only migrating keratinocytes expressed MMP-1, suggesting that contact with collagen is not sufficient to induce MMP-1 expression in keratinocytes; the cells must also be migrating. Upon denaturation, type I collagen lost its ability to induce MMP-1 expression but still supported cell adhesion. Other dermal or wound matrix proteins, such as type III collagen, fibrin, and fibronectin, and a mixture of basement membrane proteins did not induce MMP-1 production. In the presence of collagen, laminin-1 inhibited induction of MMP-1 but laminin-5 did not. Taken together, these observations suggest that as basal keratinocytes migrate from the basal lamina onto the dermal matrix contact with native type I collagen induces MMP-1 expression. In addition, our findings suggest that re-establishment of the basement membrane and, in particular, contact with laminin-1 provides a potent signal to down-regulate MMP-1 production as the epithelium is repaired.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. Zigrino, J. Steiger, J. W. Fox, S. Loffek, A. Schild, R. Nischt, and C. Mauch Role of ADAM-9 Disintegrin-Cysteine-rich Domains in Human Keratinocyte Migration J. Biol. Chem., October 19, 2007; 282(42): 30785 - 30793. [Abstract] [Full Text] [PDF]

				E. E. Gabison, S. Mourah, E. Steinfels, L. Yan, T. Hoang-Xuan, M. A. Watsky, B. De Wever, F. Calvo, A. Mauviel, and S. Menashi Differential Expression of Extracellular Matrix Metalloproteinase Inducer (CD147) in Normal and Ulcerated Corneas: Role in Epithelio-Stromal Interactions and Matrix Metalloproteinase Induction Am. J. Pathol., January 1, 2005; 166(1): 209 - 219. [Abstract] [Full Text] [PDF]

				C.-C. Chen, F.-E Mo, and L. F. Lau The Angiogenic Factor Cyr61 Activates a Genetic Program for Wound Healing in Human Skin Fibroblasts J. Biol. Chem., December 7, 2001; 276(50): 47329 - 47337. [Abstract] [Full Text] [PDF]

				N. O. Carragher, B. Levkau, R. Ross, and E. W. Raines Degraded Collagen Fragments Promote Rapid Disassembly of Smooth Muscle Focal Adhesions That Correlates with Cleavage of pp125FAK, Paxillin, and Talin J. Cell Biol., November 1, 1999; 147(3): 619 - 630. [Abstract] [Full Text] [PDF]

				M. Zhang, R. A. Pierce, H. Wachi, R. P. Mecham, and W. C. Parks An Open Reading Frame Element Mediates Posttranscriptional Regulation of Tropoelastin and Responsiveness to Transforming Growth Factor beta 1 Mol. Cell. Biol., November 1, 1999; 19(11): 7314 - 7326. [Abstract] [Full Text] [PDF]

				Y. Sun, Y. Sun, L. Wenger, J. L. Rutter, C. E. Brinckerhoff, and H. S. Cheung p53 Down-regulates Human Matrix Metalloproteinase-1 (Collagenase-1) Gene Expression J. Biol. Chem., April 23, 1999; 274(17): 11535 - 11540. [Abstract] [Full Text] [PDF]

				B. K. Pilcher, J. Dumin, M. J. Schwartz, B. A. Mast, G. S. Schultz, W. C. Parks, and H. G. Welgus Keratinocyte Collagenase-1 Expression Requires an Epidermal Growth Factor Receptor Autocrine Mechanism J. Biol. Chem., April 9, 1999; 274(15): 10372 - 10381. [Abstract] [Full Text] [PDF]

				Q. Yu and I. Stamenkovic Localization of matrix metalloproteinase 9 to the cell surface provides a mechanism for CD44-mediated tumor invasion Genes & Dev., January 1, 1999; 13(1): 35 - 48. [Abstract] [Full Text]

				J. Lohi, C. L. Wilson, J. D. Roby, and W. C. Parks Epilysin, a Novel Human Matrix Metalloproteinase (MMP-28) Expressed in Testis and Keratinocytes and in Response to Injury J. Biol. Chem., March 23, 2001; 276(13): 10134 - 10144. [Abstract] [Full Text] [PDF]

				S. L. Raza, L. C. Nehring, S. D. Shapiro, and L. A. Cornelius Proteinase-activated Receptor-1 Regulation of Macrophage Elastase (MMP-12) Secretion by Serine Proteinases J. Biol. Chem., December 22, 2000; 275(52): 41243 - 41250. [Abstract] [Full Text] [PDF]

				B. P. Nguyen, S. G. Gil, and W. G. Carter Deposition of Laminin 5 by Keratinocytes Regulates Integrin Adhesion and Signaling J. Biol. Chem., October 6, 2000; 275(41): 31896 - 31907. [Abstract] [Full Text] [PDF]

				C.-C. Chen, N. Chen, and L. F. Lau The Angiogenic Factors Cyr61 and Connective Tissue Growth Factor Induce Adhesive Signaling in Primary Human Skin Fibroblasts J. Biol. Chem., March 23, 2001; 276(13): 10443 - 10452. [Abstract] [Full Text] [PDF]

				K. D. Spradling, A. E. McDaniel, J. Lohi, and B. K. Pilcher Epsin 3 Is a Novel Extracellular Matrix-induced Transcript Specific to Wounded Epithelia J. Biol. Chem., July 27, 2001; 276(31): 29257 - 29267. [Abstract] [Full Text] [PDF]

				J. A. Dumin, S. K. Dickeson, T. P. Stricker, M. Bhattacharyya-Pakrasi, J. D. Roby, S. A. Santoro, and W. C. Parks Pro-collagenase-1 (Matrix Metalloproteinase-1) Binds the alpha 2beta 1 Integrin upon Release from Keratinocytes Migrating on Type I Collagen J. Biol. Chem., July 27, 2001; 276(31): 29368 - 29374. [Abstract] [Full Text] [PDF]