JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McKinsey, T. A.
Right arrow Articles by Ballard, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McKinsey, T. A.
Right arrow Articles by Ballard, D. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 272, Number 36, Issue of September 5, 1997 pp. 22377-22380
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

COMMUNICATION:
Phosphorylation of the PEST Domain of Ikappa Bbeta Regulates the Function of NF-kappa B/Ikappa Bbeta Complexes

(Received for publication, June 13, 1997, and in revised form, July 8, 1997)

Timothy A. McKinsey , Zhi-Liang Chu and Dean W. Ballard

From the Howard Hughes Medical Institute, Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

Activation of transcription factor NF-kappa B involves the signal-dependent degradation of basally phosphorylated inhibitors such as Ikappa Balpha and Ikappa Bbeta . The gene encoding Ikappa Balpha is under NF-kappa B control, which provides a negative feedback loop to terminate the induced NF-kappa B response. However, recent studies have identified a hypophosphorylated pool of Ikappa Bbeta that shields nuclear NF-kappa B from inhibition by newly synthesized Ikappa Balpha . In the present work, we provide three lines of evidence indicating that this protection mechanism is regulated by the C-terminal PEST domain of Ikappa Bbeta . First, disruption of two basal phosphoacceptors present in the Ikappa Bbeta PEST domain (Ser-313 and Ser-315) yields a mutant that forms ternary complexes with NF-kappa B and its target DNA-binding site. Second, based on in vitro mixing experiments, these ternary complexes are resistant to the inhibitory action of Ikappa Balpha . Third, mutants of Ikappa Bbeta that are defective for phosphorylation at Ser-313 and Ser-315 fail to efficiently block NF-kappa B-directed transcription in vivo, whereas replacement of these two Ikappa Bbeta residues with a phosphoserine mimetic generates a fully functional repressor. Taken together, our findings suggest that the functional fate of NF-kappa B when bound to Ikappa Bbeta is critically dependent on the phosphorylation status of the Ikappa Bbeta PEST domain.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
E. Hertlein, J. Wang, K. J. Ladner, N. Bakkar, and D. C. Guttridge
RelA/p65 Regulation of I{kappa}B{beta}
Mol. Cell. Biol., June 15, 2005; 25(12): 4956 - 4968.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Chen, J. Wu, and G. Ghosh
{kappa}B-Ras Binds to the Unique Insert within the Ankyrin Repeat Domain of I{kappa}B{beta} and Regulates Cytoplasmic Retention of I{kappa}B{beta}{middle dot}NF-{kappa}B Complexes
J. Biol. Chem., June 13, 2003; 278(25): 23101 - 23106.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
G. Biswas, H. K. Anandatheerthavarada, M. Zaidi, and N. G. Avadhani
Mitochondria to nucleus stress signaling: a distinctive mechanism of NF{kappa}B/Rel activation through calcineurin-mediated inactivation of I{kappa}B{beta}
J. Cell Biol., May 12, 2003; 161(3): 507 - 519.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. Basheer, E. Arrigoni, H. S. Thatte, R. W. Greene, I. S. Ambudkar, and R. W. McCarley
Adenosine Induces Inositol 1,4,5-Trisphosphate Receptor-Mediated Mobilization of Intracellular Calcium Stores in Basal Forebrain Cholinergic Neurons
J. Neurosci., September 1, 2002; 22(17): 7680 - 7686.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-H. Lee and M. Hannink
Characterization of the Nuclear Import and Export Functions of Ikappa Bepsilon
J. Biol. Chem., June 21, 2002; 277(26): 23358 - 23366.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. W. Finn, J. R. Stone, M. R. Boothby, and D. L. Perkins
Inhibition of NF-{kappa}B-Dependent T Cell Activation Abrogates Acute Allograft Rejection
J. Immunol., November 15, 2001; 167(10): 5994 - 6001.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. D. Wallace and J. A. Cidlowski
Proteasome-mediated Glucocorticoid Receptor Degradation Restricts Transcriptional Signaling by Glucocorticoids
J. Biol. Chem., November 9, 2001; 276(46): 42714 - 42721.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
D. M. Rothwarf and M. Karin
The NF-{kappa}B Activation Pathway: A Paradigm in Information Transfer from Membrane to Nucleus
Sci. Signal., October 26, 1999; 1999(5): re1 - re1.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. R. Newton, N. M. Patel, P. Bhat-Nakshatri, C. R. Stauss, R. J. Goulet Jr., and H. Nakshatri
Negative Regulation of Transactivation Function but Not DNA Binding of NF-kappa B and AP-1 by Ikappa Bbeta 1 in Breast Cancer Cells
J. Biol. Chem., June 25, 1999; 274(26): 18827 - 18835.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. DeLuca, L. Petropoulos, D. Zmeureanu, and J. Hiscott
Nuclear Ikappa Bbeta Maintains Persistent NF-kappa B Activation in HIV-1-infected Myeloid Cells
J. Biol. Chem., May 7, 1999; 274(19): 13010 - 13016.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Feinman, J. Koury, M. Thames, B. Barlogie, J. Epstein, and D. S. Siegel
Role of NF-kappa B in the Rescue of Multiple Myeloma Cells From Glucocorticoid-Induced Apoptosis by Bcl-2
Blood, May 1, 1999; 93(9): 3044 - 3052.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. M. Poppers, P. Schwenger, and J. Vilcek
Persistent Tumor Necrosis Factor Signaling in Normal Human Fibroblasts Prevents the Complete Resynthesis of Ikappa B-alpha
J. Biol. Chem., September 15, 2000; 275(38): 29587 - 29593.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Huxford, S. Malek, and G. Ghosh
Preparation and Crystallization of Dynamic NF-kappa B{middle dot}Ikappa B Complexes
J. Biol. Chem., October 13, 2000; 275(42): 32800 - 32806.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Bourke, E. J. Kennedy, and P. N. Moynagh
Loss of Ikappa B-beta Is Associated with Prolonged NF-kappa B Activity in Human Glial Cells
J. Biol. Chem., December 15, 2000; 275(51): 39996 - 40002.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Malek, Y. Chen, T. Huxford, and G. Ghosh
Ikappa Bbeta , but Not Ikappa Balpha , Functions as a Classical Cytoplasmic Inhibitor of NF-kappa B Dimers by Masking Both NF-kappa B Nuclear Localization Sequences in Resting Cells
J. Biol. Chem., November 21, 2001; 276(48): 45225 - 45235.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.