JBC Connect with Cosmo for Collagen Detection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Falkenberg, M.
Right arrow Articles by Lehman, I. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Falkenberg, M.
Right arrow Articles by Lehman, I. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 272, Number 36, Issue of September 5, 1997 pp. 22766-22770
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

The UL8 Subunit of the Heterotrimeric Herpes Simplex Virus Type 1 Helicase-Primase Is Required for the Unwinding of Single Strand DNA-binding Protein (ICP8)-coated DNA Substrates

(Received for publication, March 31, 1997, and in revised form, June 25, 1997)

Maria Falkenberg Dagger , David A. Bushnell par , Per Elias and I. R. Lehman Dagger

From the Dagger  Departments of Biochemistry and par  Structural Biology, Stanford University School of Medicine, Stanford, California 94305-5307 and  Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9, S-413 90 Göteborg, Sweden

The Herpes simplex virus type 1 primosome consists of three subunits that are the products of the UL5, UL8, and UL52 genes. The heterotrimeric enzyme has DNA-dependent ATPase, helicase, and primase activities. Earlier studies show that a subassembly consisting of the UL5 and UL52 gene products was indistinguishable from the heterotrimeric enzyme in its helicase and primase activities. We demonstrate here that the UL8 protein is required for the helicase activity of the UL5/52 subassembly on long duplex DNA substrates (>30 nucleotides) with a single-stranded DNA loading site fully coated with the virus-encoded single strand DNA binding protein, ICP8. The Escherichia coli single strand DNA binding protein cannot substitute for ICP8, suggesting a specific physical interaction between ICP8 and the UL8 protein. Surface plasmon resonance measurements demonstrated an interaction between ICP8 and the UL5/52/8 heterotrimer but not with the UL5/52 subassembly or the UL8 protein alone. At a subsaturating level of ICP8, the UL5/52 subassembly does show helicase activity, suggesting that the subassembly can bind to single-stranded DNA but not to ICP8-coated DNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
Y. Chen, C. M. Livingston, S. D. Carrington-Lawrence, P. Bai, and S. K. Weller
A Mutation in the Human Herpes Simplex Virus Type 1 UL52 Zinc Finger Motif Results in Defective Primase Activity but Can Recruit Viral Polymerase and Support Viral Replication Efficiently
J. Virol., August 15, 2007; 81(16): 8742 - 8751.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y. Chen, S. D. Carrington-Lawrence, P. Bai, and S. K. Weller
Mutations in the Putative Zinc-Binding Motif of UL52 Demonstrate a Complex Interdependence between the UL5 and UL52 Subunits of the Human Herpes Simplex Virus Type 1 Helicase/Primase Complex
J. Virol., July 15, 2005; 79(14): 9088 - 9096.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. V. Nimonkar and P. E. Boehmer
Role of Protein-Protein Interactions during Herpes Simplex Virus Type 1 Recombination-dependent Replication
J. Biol. Chem., May 21, 2004; 279(21): 21957 - 21965.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Korhonen, M. Gaspari, and M. Falkenberg
TWINKLE Has 5' -> 3' DNA Helicase Activity and Is Specifically Stimulated by Mitochondrial Single-stranded DNA-binding Protein
J. Biol. Chem., December 5, 2003; 278(49): 48627 - 48632.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. Ward, P. Elias, and R. M. Linden
Rescue of the Adeno-Associated Virus Genome from a Plasmid Vector: Evidence for Rescue by Replication
J. Virol., November 1, 2003; 77(21): 11480 - 11490.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. V. Nimonkar and P. E. Boehmer
Reconstitution of recombination-dependent DNA synthesis in herpes simplex virus 1
PNAS, September 2, 2003; 100(18): 10201 - 10206.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. D. Carrington-Lawrence and S. K. Weller
Recruitment of Polymerase to Herpes Simplex Virus Type 1 Replication Foci in Cells Expressing Mutant Primase (UL52) Proteins
J. Virol., April 1, 2003; 77(7): 4237 - 4247.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Gascon, J. L. Carrascosa, L. Villar, J. M. Lazaro, and M. Salas
Importance of the N-terminal Region of the Phage GA-1 Single-stranded DNA-binding Protein for Its Self-interaction Ability and Functionality
J. Biol. Chem., June 14, 2002; 277(25): 22534 - 22540.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. V. Nimonkar and P. E. Boehmer
In Vitro Strand Exchange Promoted by the Herpes Simplex Virus Type-1 Single Strand DNA-binding Protein (ICP8) and DNA Helicase-Primase
J. Biol. Chem., April 19, 2002; 277(17): 15182 - 15189.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. Ward, M. Falkenberg, P. Elias, M. Weitzman, and R. M. Linden
Rep-Dependent Initiation of Adeno-Associated Virus Type 2 DNA Replication by a Herpes Simplex Virus Type 1 Replication Complex in a Reconstituted System
J. Virol., November 1, 2001; 75(21): 10250 - 10258.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Falkenberg, I. R. Lehman, and P. Elias
Leading and lagging strand DNA synthesis in vitro by a reconstituted herpes simplex virus type 1 replisome
PNAS, April 11, 2000; 97(8): 3896 - 3900.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. R. Lehman and P. E. Boehmer
Replication of Herpes Simplex Virus DNA
J. Biol. Chem., October 1, 1999; 274(40): 28059 - 28062.
[Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
N. Constantin and M. S. Dodson
Two-hybrid analysis of the interaction between the UL52 and UL8 subunits of the herpes simplex virus type 1 helicase-primase
J. Gen. Virol., September 1, 1999; 80(9): 2411 - 2415.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N. Biswas and S. K. Weller
A Mutation in the C-terminal Putative Zn2+ Finger Motif of UL52 Severely Affects the Biochemical Activities of the HSV-1 Helicase-Primase Subcomplex
J. Biol. Chem., March 19, 1999; 274(12): 8068 - 8076.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Falkenberg, P. Elias, and I. R. Lehman
The Herpes Simplex Virus Type 1 Helicase-primase. ANALYSIS OF HELICASE ACTIVITY
J. Biol. Chem., November 27, 1998; 273(48): 32154 - 32157.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
F. C. Spector, L. Liang, H. Giordano, M. Sivaraja, and M. G. Peterson
Inhibition of Herpes Simplex Virus Replication by a 2-Amino Thiazole via Interactions with the Helicase Component of the UL5-UL8-UL52 Complex
J. Virol., September 1, 1998; 72(9): 6979 - 6987.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. T. Le Gac, G. Villani, and P. E. Boehmer
Herpes Simplex Virus Type-1 Single-strand DNA-binding Protein (ICP8) Enhances the Ability of the Viral DNA Helicase-primase to Unwind Cisplatin-modified DNA
J. Biol. Chem., May 29, 1998; 273(22): 13801 - 13807.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. E. Boehmer
The Herpes Simplex Virus Type-1 Single-strand DNA-binding Protein, ICP8, Increases the Processivity of the UL9 Protein DNA Helicase
J. Biol. Chem., January 30, 1998; 273(5): 2676 - 2683.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.