Volume 272, Number 37, Issue of September 12, 1997 pp. 22991-22994
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

COMMUNICATION:
DNA Damage Recognition by XPA Protein Promotes Efficient Recruitment of Transcription Factor II H

(Received for publication, June 2, 1997, and in revised form, July 20, 1997)

Silvano Nocentini , Frédéric Coin ^¶ , Masafumi Saijo , Kiyoji Tanaka and Jean-Marc Egly ^¶

From the  CNRS UMR 218 et LRC no. 1 du CEA, Institut Curie, Section de Recherche, 26 rue d'Ulm, 75248 Paris Cedex 05, France, the ^¶ Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM, 1 rue Laurent Fries, B. P. 163, 67404 Illkirch Cedex, CU de Strasbourg, France, and the  Division of Cellular Genetics, Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, Japan

The human basal transcription factor IIH (TFIIH) is an essential component of the nucleotide excision repair machinery. TFIIH is required for reaction steps concomitant with or prior to the formation of dual incisions in the damaged DNA strand. To understand the mechanism underlying the recruitment of TFIIH to DNA damage sites we have analyzed i) the direct affinity of TFIIH for damaged or undamaged DNA and ii) the interaction of TFIIH with XPA·DNA complexes, formed using unirradiated or UV-irradiated DNA.

Filter binding assays showed that TFIIH has some affinity for the DNA, but in contrast to XPA, does not show any preference for UV-irradiated DNA. Pull-down experiments demonstrated that TFIIH binds to XPA·DNA complexes in an UV damage-dependent manner by a direct protein-protein interaction with XPA. We propose that an enhancement of the affinity of XPA protein for TFIIH could arise from conformational changes of XPA when it binds to UV lesions on the DNA.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Q.-E. Wang, Q. Zhu, G. Wani, J. Chen, and A. A. Wani UV radiation-induced XPC translocation within chromatin is mediated by damaged-DNA binding protein, DDB2 Carcinogenesis, June 1, 2004; 25(6): 1033 - 1043. [Abstract] [Full Text] [PDF]

				F. Guillonneau, A. L. Guieysse, S. Nocentini, C. Giovannangeli, and D. Praseuth Psoralen interstrand cross-link repair is specifically altered by an adjacent triple-stranded structure Nucleic Acids Res., February 13, 2004; 32(3): 1143 - 1153. [Abstract] [Full Text] [PDF]

				Z. Chen, X. S. Xu, J. Yang, and G. Wang Defining the function of XPC protein in psoralen and cisplatin-mediated DNA repair and mutagenesis Carcinogenesis, June 1, 2003; 24(6): 1111 - 1121. [Abstract] [Full Text] [PDF]

				J. T. Reardon and A. Sancar Molecular Anatomy of the Human Excision Nuclease Assembled at Sites of DNA Damage Mol. Cell. Biol., August 15, 2002; 22(16): 5938 - 5945. [Abstract] [Full Text] [PDF]

				S. J. Araújo, E. A. Nigg, and R. D. Wood Strong Functional Interactions of TFIIH with XPC and XPG in Human DNA Nucleotide Excision Repair, without a Preassembled Repairosome Mol. Cell. Biol., April 1, 2001; 21(7): 2281 - 2291. [Abstract] [Full Text]

				K. Sugasawa, T. Okamoto, Y. Shimizu, C. Masutani, S. Iwai, and F. Hanaoka A multistep damage recognition mechanism for global genomic nucleotide excision repair Genes & Dev., March 1, 2001; 15(5): 507 - 521. [Abstract] [Full Text]

				M. Nitta, M. Saijo, N. Kodo, T. Matsuda, Y. Nakatsu, H. Tamai, and K. Tanaka A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein XPA Nucleic Acids Res., November 1, 2000; 28(21): 4212 - 4218. [Abstract] [Full Text] [PDF]

				D. GOUKASSIAN, F. GAD, M. YAAR, M. S. ELLER, U. S. NEHAL, and B. A. GILCHREST Mechanisms and implications of the age-associated decrease in DNA repair capacity FASEB J, July 1, 2000; 14(10): 1325 - 1334. [Abstract] [Full Text]

				M. Yokoi, C. Masutani, T. Maekawa, K. Sugasawa, Y. Ohkuma, and F. Hanaoka The Xeroderma Pigmentosum Group C Protein Complex XPC-HR23B Plays an Important Role in the Recruitment of Transcription Factor IIH to Damaged DNA J. Biol. Chem., March 24, 2000; 275(13): 9870 - 9875. [Abstract] [Full Text] [PDF]

				M. Wakasugi and A. Sancar Order of Assembly of Human DNA Repair Excision Nuclease J. Biol. Chem., June 25, 1999; 274(26): 18759 - 18768. [Abstract] [Full Text] [PDF]

				W. L. de Laat, N. G.J. Jaspers, and J. H.J. Hoeijmakers Molecular mechanism of nucleotide excision repair Genes & Dev., April 1, 1999; 13(7): 768 - 785. [Full Text]

				S. T. Szak and J. A. Pietenpol High Affinity Insertion/Deletion Lesion Binding by p53. EVIDENCE FOR A ROLE OF THE p53 CENTRAL DOMAIN J. Biol. Chem., February 5, 1999; 274(6): 3904 - 3909. [Abstract] [Full Text] [PDF]

				D. Tantin RNA Polymerase II Elongation Complexes Containing the Cockayne Syndrome Group B Protein Interact with a Molecular Complex Containing the Transcription Factor IIH Components Xeroderma Pigmentosum B and p62 J. Biol. Chem., October 23, 1998; 273(43): 27794 - 27799. [Abstract] [Full Text] [PDF]

				F. Coin, P. Frit, B. Viollet, B. Salles, and J.-M. Egly TATA Binding Protein Discriminates between Different Lesions on DNA, Resulting in a Transcription Decrease Mol. Cell. Biol., July 1, 1998; 18(7): 3907 - 3914. [Abstract] [Full Text]

				M. Wakasugi and A. Sancar Assembly, subunit composition, and footprint of human DNA repair excision nuclease PNAS, June 9, 1998; 95(12): 6669 - 6674. [Abstract] [Full Text] [PDF]