| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, April 11, 1997, and in revised form, June 4, 1997)
From the Department of Immunology, IMM-12, The Scripps Research
Institute, La Jolla, California 92037
The stimulation of nonmyeloid cells by
lipopolysaccharide (LPS) is mediated by the serum protein, soluble CD14
(sCD14). We have examined the interaction of sCD14 with whole cells
using a biologically active radiolabeled sCD14 molecule as a ligand. Specific binding of sCD14 to nonmyeloid cells is detected only when it
is first incubated with both LPS and the serum LPS-binding protein
(LBP). Through the use of an anti-CD14 monoclonal antibody, we
demonstrate that sCD14 must interact with LPS in order for cellular
binding to occur. Although LBP is traditionally known to function as a
catalyst in the transfer of LPS to sCD14, our results reveal that LBP
is actually a physical part of sCD14-containing, cell-associating
complexes. The LPS- and LBP-dependent cell surface binding
of sCD14 appears to be distinct from events leading to cell
stimulation, since certain anti-CD14 and anti-LBP monoclonal antibodies
have different effects on cellular binding versus cellular activation. Bound sCD14 is internalized, indicating that the LBP- and
LPS-dependent binding of sCD14 may represent a novel
general mechanism by which nonmyeloid cells clear LPS.
Volume 272, Number 37,
Issue of September 12, 1997
pp. 23157-23164
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. O. Omueti, J. M. Beyer, C. M. Johnson, E. A. Lyle, and R. I. Tapping Domain Exchange between Human Toll-like Receptors 1 and 6 Reveals a Region Required for Lipopeptide Discrimination J. Biol. Chem., November 4, 2005; 280(44): 36616 - 36625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Teghanemt, D. Zhang, E. N. Levis, J. P. Weiss, and T. L. Gioannini Molecular Basis of Reduced Potency of Underacylated Endotoxins J. Immunol., October 1, 2005; 175(7): 4669 - 4676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.L. Gioannini, A. Teghanemt, DeS. Zhang, E.N. Levis, and J.P. Weiss Monomeric endotoxin:protein complexes are essential for TLR4-dependent cell activation Innate Immunity, April 1, 2005; 11(2): 117 - 123. [Abstract] [PDF]
|
|
|
|
|
|
R. W. Finberg, F. Re, L. Popova, D. T. Golenbock, and E. A. Kurt-Jones Cell activation by Toll-like receptors: role of LBP and CD14 Innate Immunity, December 1, 2004; 10(6): 413 - 418. [Abstract] [PDF]
|
|
|
|
 |
|
 F. Vangroenweghe, P. Rainard, M. Paape, L. Duchateau, and C. Burvenich Increase of Escherichia coli Inoculum Doses Induces Faster Innate Immune Response in Primiparous Cows J Dairy Sci, December 1, 2004; 87(12): 4132 - 4144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Bannerman, M. J. Paape, W. R. Hare, and J. C. Hope Characterization of the Bovine Innate Immune Response to Intramammary Infection with Klebsiella pneumoniae J Dairy Sci, August 1, 2004; 87(8): 2420 - 2432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dunzendorfer, H.-K. Lee, K. Soldau, and P. S. Tobias TLR4 Is the Signaling but Not the Lipopolysaccharide Uptake Receptor J. Immunol., July 15, 2004; 173(2): 1166 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Roman, J. D. Ritzenthaler, B. Boles, M. Lois, and S. Roser-Page Lipopolysaccharide induces expression of fibronectin {alpha}5{beta}1-integrin receptors in human monocytic cells in a protein kinase C-dependent fashion Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L239 - L249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Gioannini, A. Teghanemt, D. Zhang, N. P. Coussens, W. Dockstader, S. Ramaswamy, and J. P. Weiss Isolation of an endotoxin-MD-2 complex that produces Toll-like receptor 4-dependent cell activation at picomolar concentrations PNAS, March 23, 2004; 101(12): 4186 - 4191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Gioannini, A. Teghanemt, K. A. Zarember, and J. P. Weiss Regulation of interactions of endotoxin with host cells Innate Immunity, December 1, 2003; 9(6): 401 - 408. [Abstract] [PDF]
|
|
|
|
|
|
D. D. Bannerman, M. J. Paape, W. R. Hare, and E. J. Sohn Increased Levels of LPS-Binding Protein in Bovine Blood and Milk Following Bacterial Lipopolysaccharide Challenge J Dairy Sci, October 1, 2003; 86(10): 3128 - 3137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Thompson, P. S. Tobias, S. Viriyakosol, T. N. Kirkland, and R. L. Kitchens Lipopolysaccharide (LPS)-binding Protein Inhibits Responses to Cell-bound LPS J. Biol. Chem., August 1, 2003; 278(31): 28367 - 28371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Schilling, S. M. Martin, D. A. Hunstad, K. P. Patel, M. A. Mulvey, S. S. Justice, R. G. Lorenz, and S. J. Hultgren CD14- and Toll-Like Receptor-Dependent Activation of Bladder Epithelial Cells by Lipopolysaccharide and Type 1 Piliated Escherichia coli Infect. Immun., March 1, 2003; 71(3): 1470 - 1480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Bannerman, J. C. Tupper, R. D. Erwert, R. K. Winn, and J. M. Harlan Divergence of Bacterial Lipopolysaccharide Pro-apoptotic Signaling Downstream of IRAK-1 J. Biol. Chem., March 1, 2002; 277(10): 8048 - 8053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Hasunuma, H. Morita, S. Tanaka, R. Ryll, M. A. Freudenberg, C. Galanos, and Y. Kumazawa Differential clearance and induction of host responses by various administered or released lipopolysaccharides Innate Immunity, December 1, 2001; 7(6): 421 - 429. [Abstract] [PDF]
|
|
|
|
|
|
H. Chiba, H. Sano, D. Iwaki, S. Murakami, H. Mitsuzawa, T. Takahashi, M. Konishi, H. Takahashi, and Y. Kuroki Rat Mannose-Binding Protein A Binds CD14 Infect. Immun., March 1, 2001; 69(3): 1587 - 1592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Baveye, E. Elass, D. G. Fernig, C. Blanquart, J. Mazurier, and D. Legrand Human Lactoferrin Interacts with Soluble CD14 and Inhibits Expression of Endothelial Adhesion Molecules, E-Selectin and ICAM-1, Induced by the CD14-Lipopolysaccharide Complex Infect. Immun., December 1, 2000; 68(12): 6519 - 6525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dziarski, S. Viriyakosol, T. N. Kirkland, and D. Gupta Soluble CD14 Enhances Membrane CD14-Mediated Responses to Peptidoglycan: Structural Requirements Differ from Those for Responses to Lipopolysaccharide Infect. Immun., September 1, 2000; 68(9): 5254 - 5260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Muzio, D. Bosisio, N. Polentarutti, G. D'amico, A. Stoppacciaro, R. Mancinelli, C. van't Veer, G. Penton-Rol, L. P. Ruco, P. Allavena, et al. Differential Expression and Regulation of Toll-Like Receptors (TLR) in Human Leukocytes: Selective Expression of TLR3 in Dendritic Cells J. Immunol., June 1, 2000; 164(11): 5998 - 6004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. I. Tapping, S. L. Orr, and P. S. Tobias LBP does not substitute for CD14 as a signaling membrane bound LPS receptor Innate Immunity, June 1, 1999; 5(3): 164 - 166. [Abstract] [PDF]
|
|
|
|
|
|
R. I. Tapping, S. L. Orr, E. M. Lawson, K. Soldau, and P. S. Tobias Membrane-Anchored Forms of Lipopolysaccharide (LPS)-Binding Protein Do Not Mediate Cellular Responses to LPS Independently of CD14 J. Immunol., May 1, 1999; 162(9): 5483 - 5489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Wright Toll, A New Piece in the Puzzle of Innate Immunity J. Exp. Med., February 15, 1999; 189(4): 605 - 609. [Full Text] [PDF]
|
|
|
|
|
|
R. I. Tapping and P. S. Tobias Cell surface binding of LBP--LPS complexes to a protein component distinct from CD14 Innate Immunity, February 1, 1999; 5(1-2): 52 - 55. [Abstract] [PDF]
|
|
|
|
|
|
R. Dziarski and D. Gupta Function of CD14 as a peptidoglycan receptor: differences and similarities with LPS Innate Immunity, February 1, 1999; 5(1-2): 56 - 61. [Abstract] [PDF]
|
|
|
|
|
|
R. Dziarski, R. I. Tapping, and P. S. Tobias Binding of Bacterial Peptidoglycan to CD14 J. Biol. Chem., April 10, 1998; 273(15): 8680 - 8690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sano, H. Chiba, D. Iwaki, H. Sohma, D. R. Voelker, and Y. Kuroki Surfactant Proteins A and D Bind CD14 by Different Mechanisms J. Biol. Chem., July 14, 2000; 275(29): 22442 - 22451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Giardina, T. Gioannini, B. A. Buscher, A. Zaleski, D.-S. Zheng, L. Stoll, A. Teghanemt, M. A. Apicella, and J. Weiss Construction of Acetate Auxotrophs of Neisseria meningitidis to Study Host-Meningococcal Endotoxin Interactions J. Biol. Chem., February 16, 2001; 276(8): 5883 - 5891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Mitsuzawa, I. Wada, H. Sano, D. Iwaki, S. Murakami, T. Himi, N. Matsushima, and Y. Kuroki Extracellular Toll-Like Receptor 2 Region Containing Ser40-Ile64 but Not Cys30-Ser39 Is Critical for the Recognition of Staphylococcus aureus Peptidoglycan J. Biol. Chem., October 26, 2001; 276(44): 41350 - 41356. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
