HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thyagarajan, B. Articles by Campbell, C. Search for Related Content PubMed PubMed Citation Articles by Thyagarajan, B. Articles by Campbell, C. Social Bookmarking                      What's this?

Volume 272, Number 37, Issue of September 12, 1997 pp. 23328-23333
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Elevated Homologous Recombination Activity in Fanconi Anemia Fibroblasts

(Received for publication, February 19, 1997, and in revised form, July 7, 1997)

From the Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455

It is widely believed that Fanconi anemia cells possess a reduced ability to repair inter-strand DNA cross-links. While the mechanism through which inter-strand DNA cross-links are removed from mammalian chromosomes is unknown, these lesions are repaired via homologous recombination in lower eukaryotes and bacteria. Based on the hypothesis that a similar mechanism of DNA repair functions in mammalian somatic cells, we measured homologous recombination activity in diploid fibroblasts from healthy donors, and Fanconi anemia patients. Somewhat surprisingly, homologous recombination levels in nuclear protein extracts prepared from Fanconi anemia cells were nearly 100-fold higher than in extracts prepared from control cells. We observed a similar increase in the activity of a 100-kDa homologous DNA pairing protein in extracts from Fanconi anemia cells. Transfection studies confirmed that plasmid homologous recombination levels in intact Fanconi anemia cells were substantially elevated, compared with control cells. These results suggest that inappropriately elevated levels of homologous recombination activity may contribute to the genomic instability and cancer predisposition that characterize Fanconi anemia.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. M. Hinz, P. B. Nham, S. S. Urbin, I. M. Jones, and L. H. Thompson Disparate contributions of the Fanconi anemia pathway and homologous recombination in preventing spontaneous mutagenesis Nucleic Acids Res., June 28, 2007; 35(11): 3733 - 3740. [Abstract] [Full Text] [PDF]

				A. Rothfuss and M. Grompe Repair Kinetics of Genomic Interstrand DNA Cross-Links: Evidence for DNA Double-Strand Break-Dependent Activation of the Fanconi Anemia/BRCA Pathway Mol. Cell. Biol., January 1, 2004; 24(1): 123 - 134. [Abstract] [Full Text] [PDF]

				J. C.Y. Wong, N. Alon, C. Mckerlie, J. R. Huang, M. S. Meyn, and M. Buchwald Targeted disruption of exons 1 to 6 of the Fanconi Anemia group A gene leads to growth retardation, strain-specific microphthalmia, meiotic defects and primordial germ cell hypoplasia Hum. Mol. Genet., August 15, 2003; 12(16): 2063 - 2076. [Abstract] [Full Text] [PDF]

				S. L. Donahue, R. Lundberg, R. Saplis, and C. Campbell Deficient Regulation of DNA Double-strand Break Repair in Fanconi Anemia Fibroblasts J. Biol. Chem., August 8, 2003; 278(32): 29487 - 29495. [Abstract] [Full Text] [PDF]

				A. R. Meetei, S. Sechi, M. Wallisch, D. Yang, M. K. Young, H. Joenje, M. E. Hoatlin, and W. Wang A Multiprotein Nuclear Complex Connects Fanconi Anemia and Bloom Syndrome Mol. Cell. Biol., May 15, 2003; 23(10): 3417 - 3426. [Abstract] [Full Text] [PDF]

				M. Bogliolo, O. Cabre, E. Callen, V. Castillo, A. Creus, R. Marcos, and J. Surralles The Fanconi anaemia genome stability and tumour suppressor network Mutagenesis, November 1, 2002; 17(6): 529 - 538. [Abstract] [Full Text] [PDF]

				P. Pichierri, D. Averbeck, and F. Rosselli DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein Hum. Mol. Genet., October 2, 2002; 11(21): 2531 - 2546. [Abstract] [Full Text] [PDF]

				M. Digweed, S. Rothe, I. Demuth, R. Scholz, D. Schindler, M. Stumm, M. Grompe, A. Jordan, and K. Sperling Attenuation of the formation of DNA-repair foci containing RAD51 in Fanconi anaemia Carcinogenesis, July 1, 2002; 23(7): 1121 - 1126. [Abstract] [Full Text] [PDF]

				M. Grompe and A. D'Andrea Fanconi anemia and DNA repair Hum. Mol. Genet., October 1, 2001; 10(20): 2253 - 2259. [Abstract] [Full Text] [PDF]

				T. Hoshino, J. Wang, M. P. Devetten, N. Iwata, S. Kajigaya, R. J. Wise, J. M. Liu, and H. Youssoufian Molecular Chaperone GRP94 Binds to the Fanconi Anemia Group C Protein and Regulates Its Intracellular Expression Blood, June 1, 1998; 91(11): 4379 - 4386. [Abstract] [Full Text] [PDF]

				R. Lundberg, M. Mavinakere, and C. Campbell Deficient DNA End Joining Activity in Extracts from Fanconi Anemia Fibroblasts J. Biol. Chem., March 16, 2001; 276(12): 9543 - 9549. [Abstract] [Full Text] [PDF]