HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gavva, N. R. Articles by Shen, C.-K. J. Search for Related Content PubMed PubMed Citation Articles by Gavva, N. R. Articles by Shen, C.-K. J. Social Bookmarking                      What's this?

Volume 272, Number 39, Issue of September 26, 1997 pp. 24105-24108
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

COMMUNICATION:
Interaction of WW Domains with Hematopoietic Transcription Factor p45/NF-E2 and RNA Polymerase II

(Received for publication, July 8, 1997)

Narender R. Gavva , Rama Gavva , Kira Ermekova ^§ , Marius Sudol ^§ and C.-K. James Shen ^¶

From the  Section of Molecular and Cellular Biology, University of California, Davis, California 95616, the ^§ Department of Biochemistry, Mount Sinai School of Medicine, New York, New York 10029, and the ^¶ Institute of Molecular Biology, Academia Sinica, Taipei 11529, Republic of China

NF-E2 is an erythroid-specific transcription factor required for expression of several erythroid-specific genes. By Far-Western blotting and yeast two-hybrid assay, we demonstrate that p45, the large subunit of NF-E2, is capable of binding to a specific set of WW domain-containing proteins, including the ubiquitin ligase hRPF1. This binding is mediated through the interaction between the WW domains and a PY motif located within the amino-terminal region of p45. Interestingly, the carboxyl-terminal domain of mammalian RNA polymerase II binds a similar set of WW domains to which p45 interacts with. We discuss the data in terms of possible new pathways through which the processes of transcriptional regulation by NF-E2 could be regulated in erythroid and megakaryote cells.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Bao, C. Cao, X. Zhang, F. Jiang, S. V. Nicosia, and W. Bai Suppression of {beta}-Amyloid Precursor Protein Signaling into the Nucleus by Estrogens Mediated through Complex Formation between the Estrogen Receptor and Fe65 Mol. Cell. Biol., February 15, 2007; 27(4): 1321 - 1333. [Abstract] [Full Text] [PDF]

				B. Chenais, A. Derjuga, W. Massrieh, K. Red-Horse, V. Bellingard, S. J. Fisher, and V. Blank Functional and Placental Expression Analysis of the Human NRF3 Transcription Factor Mol. Endocrinol., January 1, 2005; 19(1): 125 - 137. [Abstract] [Full Text] [PDF]

				Y. Kato, K. Nagata, M. Takahashi, L. Lian, J. J. Herrero, M. Sudol, and M. Tanokura Common Mechanism of Ligand Recognition by Group II/III WW Domains: REDEFINING THEIR FUNCTIONAL CLASSIFICATION J. Biol. Chem., July 23, 2004; 279(30): 31833 - 31841. [Abstract] [Full Text] [PDF]

				C. M. Kiekhaefer, M. E. Boyer, K. D. Johnson, and E. H. Bresnick A WW Domain-binding Motif within the Activation Domain of the Hematopoietic Transcription Factor NF-E2 Is Essential for Establishment of a Tissue-specific Histone Modification Pattern J. Biol. Chem., February 27, 2004; 279(9): 7456 - 7461. [Abstract] [Full Text] [PDF]

				M. R. Loyd, Y. Okamoto, M. S. Randall, and P. A. Ney Role of AP1/NFE2 binding sites in endogenous {alpha}-globin gene transcription Blood, December 1, 2003; 102(12): 4223 - 4228. [Abstract] [Full Text] [PDF]

				L. Otte, U. Wiedemann, B. Schlegel, J. R. Pires, M. Beyermann, P. Schmieder, G. Krause, R. Volkmer-Engert, J. Schneider-Mergener, and H. Oschkinat WW domain sequence activity relationships identified using ligand recognition propensities of 42 WW domains Protein Sci., March 1, 2003; 12(3): 491 - 500. [Abstract] [Full Text] [PDF]

				C. Francastel, W. Magis, and M. Groudine Nuclear relocation of a transactivator subunit precedes target gene activation PNAS, September 26, 2001; (2001) 211444898. [Abstract] [Full Text] [PDF]

				H. R. Jayakar, K. G. Murti, and M. A. Whitt Mutations in the PPPY Motif of Vesicular Stomatitis Virus Matrix Protein Reduce Virus Budding by Inhibiting a Late Step in Virion Release J. Virol., November 1, 2000; 74(21): 9818 - 9827. [Abstract] [Full Text]

				M. T. Bedford, D. Sarbassova, J. Xu, P. Leder, and M. B. Yaffe A Novel Pro-Arg Motif Recognized by WW Domains J. Biol. Chem., March 31, 2000; 275(14): 10359 - 10369. [Abstract] [Full Text] [PDF]

				B. K. KAY, M. P. WILLIAMSON, and M. SUDOL The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains FASEB J, February 1, 2000; 14(2): 231 - 241. [Abstract] [Full Text]

				A Veraksa, N McGinnis, X Li, J Mohler, and W McGinnis Cap 'n' collar B cooperates with a small Maf subunit to specify pharyngeal development and suppress deformed homeotic function in the Drosophila head Development, January 9, 2000; 127(18): 4023 - 4037. [Abstract] [PDF]

				D. P. Morris, H. P. Phatnani, and A. L. Greenleaf Phospho-Carboxyl-Terminal Domain Binding and the Role of a Prolyl Isomerase in Pre-mRNA 3'-End Formation J. Biol. Chem., October 29, 1999; 274(44): 31583 - 31587. [Abstract] [Full Text] [PDF]

				E. C. Forsberg, K. Johnson, T. N. Zaboikina, E. A. Mosser, and E. H. Bresnick Requirement of an E1A-sensitive Coactivator for Long-range Transactivation by the beta -Globin Locus Control Region J. Biol. Chem., September 17, 1999; 274(38): 26850 - 26859. [Abstract] [Full Text] [PDF]

				X. Espanel and M. Sudol A Single Point Mutation in a Group I WW Domain Shifts Its Specificity to That of Group II WW Domains J. Biol. Chem., June 11, 1999; 274(24): 17284 - 17289. [Abstract] [Full Text] [PDF]

				K. F. Harvey, A. Dinudom, P. Komwatana, C. N. Jolliffe, M. L. Day, G. Parasivam, D. I. Cook, and S. Kumar All Three WW Domains of Murine Nedd4 Are Involved in the Regulation of Epithelial Sodium Channels by Intracellular Na+ J. Biol. Chem., April 30, 1999; 274(18): 12525 - 12530. [Abstract] [Full Text] [PDF]

				N. J. McKenna, Z. Nawaz, S. Y. Tsai, M.-J. Tsai, and B. W. O'Malley Distinct steady-state nuclear receptor coregulator complexes exist in vivo PNAS, September 29, 1998; 95(20): 11697 - 11702. [Abstract] [Full Text] [PDF]

				W. K. Versaw, V. Blank, N. M. Andrews, and E. H. Bresnick Mitogen-activated protein kinases enhance long-range activation by the beta -globin locus control region PNAS, July 21, 1998; 95(15): 8756 - 8760. [Abstract] [Full Text] [PDF]

				K. F. Harvey, N. L. Harvey, J. M. Michael, G. Parasivam, N. Waterhouse, E. S. Alnemri, D. Watters, and S. Kumar Caspase-mediated Cleavage of the Ubiquitin-protein Ligase Nedd4 during Apoptosis J. Biol. Chem., May 29, 1998; 273(22): 13524 - 13530. [Abstract] [Full Text] [PDF]

				D. Casteel, M. Suhasini, T. Gudi, R. Naima, and R. B. Pilz Regulation of the Erythroid Transcription Factor NF-E2 by Cyclic Adenosine Monophosphate-Dependent Protein Kinase Blood, May 1, 1998; 91(9): 3193 - 3201. [Abstract] [Full Text] [PDF]

				K. P. Mitton, P. K. Swain, S. Chen, S. Xu, D. J. Zack, and A. Swaroop The Leucine Zipper of NRL Interacts with the CRX Homeodomain. A POSSIBLE MECHANISM OF TRANSCRIPTIONAL SYNERGY IN RHODOPSIN REGULATION J. Biol. Chem., September 15, 2000; 275(38): 29794 - 29799. [Abstract] [Full Text] [PDF]

				H.-L. Hung, A. Y. Kim, W. Hong, C. Rakowski, and G. A. Blobel Stimulation of NF-E2 DNA Binding by CREB-binding Protein (CBP)-mediated Acetylation J. Biol. Chem., March 30, 2001; 276(14): 10715 - 10721. [Abstract] [Full Text] [PDF]

				M. H. Hamilton, I. Tcherepanova, J. M. Huibregtse, and D. P. McDonnell Nuclear Import/Export of hRPF1/Nedd4 Regulates the Ubiquitin- dependent Degradation of Its Nuclear Substrates J. Biol. Chem., July 6, 2001; 276(28): 26324 - 26331. [Abstract] [Full Text] [PDF]

				M. T. Bedford, A. Frankel, M. B. Yaffe, S. Clarke, P. Leder, and S. Richard Arginine Methylation Inhibits the Binding of Proline-rich Ligands to Src Homology 3, but Not WW, Domains J. Biol. Chem., May 19, 2000; 275(21): 16030 - 16036. [Abstract] [Full Text] [PDF]

				S. E. Salghetti, M. Muratani, H. Wijnen, B. Futcher, and W. P. Tansey Functional overlap of sequences that activate transcription and signal ubiquitin-mediated proteolysis PNAS, March 28, 2000; 97(7): 3118 - 3123. [Abstract] [Full Text] [PDF]

				C. Francastel, W. Magis, and M. Groudine Nuclear relocation of a transactivator subunit precedes target gene activation PNAS, October 9, 2001; 98(21): 12120 - 12125. [Abstract] [Full Text] [PDF]