HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hadri, K. E. Articles by Fève, B. Search for Related Content PubMed PubMed Citation Articles by Hadri, K. E. Articles by Fève, B. Social Bookmarking                      What's this?

Volume 272, Number 39, Issue of September 26, 1997 pp. 24514-24521
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Differential Regulation by Tumor Necrosis Factor- of ₁-, ₂-, and ₃-Adrenoreceptor Gene Expression in 3T3-F442A Adipocytes

(Received for publication, April 7, 1997, and in revised form, June 27, 1997)

From INSERM Unité 282, Hôpital Henri Mondor, 94010 Créteil, France

Modulation of -adrenoreceptor expression by tumor necrosis factor- (TNF-) was investigated in murine 3T3-F442A adipocytes. TNF- treatment of mature adipocytes decreased ₃-adrenoreceptor mRNA content in a time- and concentration-dependent manner, with a 8.5-fold decrease observed after a 6-h exposure to 300 pM TNF-. ₁-Adrenoreceptor mRNA abundance was slightly decreased by TNF- treatment, while ₂-adrenoreceptor mRNA levels were potently induced (6-fold increase at 6 h). ()-[¹²⁵I]Iodocyanopindolol saturation and competition binding experiments indicated that TNF- induced a 2-fold decrease in ₃-adrenoreceptor number, a nonsignificant reduction in ₁-subtype population, and a ~4.5-fold increase in ₂-adrenoreceptor density. This correlated with a lower EC₅₀ value measured for epinephrine in stimulating adenylyl cyclase, whereas the EC₅₀ value for norepinephrine increased. Nuclear run-on assays on isolated nuclei and mRNA stability measurements showed that TNF- increased both ₂-adrenoreceptor gene transcription and ₂-adrenoreceptor mRNA half-life, while ₁- and ₃-adrenoreceptor gene expression was modulated only at the transcriptional level by the cytokine. These findings demonstrate a differential modulation by TNF- of the three -adrenoreceptor subtypes in adipocytes, which may contribute to metabolic disorders induced by the cytokine in the adipocyte.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Mercier, M. Moldes, K. E. Hadri, and B. Feve Regulation of Semicarbazide-Sensitive Amine Oxidase Expression by Tumor Necrosis Factor-alpha in Adipocytes: Functional Consequences on Glucose Transport J. Pharmacol. Exp. Ther., March 1, 2003; 304(3): 1197 - 1208. [Abstract] [Full Text] [PDF]

				M. Friedberg, E. Zoumakis, N. Hiroi, T. Bader, G. P. Chrousos, and Z.'e. Hochberg Modulation of 11{beta}-Hydroxysteroid Dehydrogenase Type 1 in Mature Human Subcutaneous Adipocytes by Hypothalamic Messengers J. Clin. Endocrinol. Metab., January 1, 2003; 88(1): 385 - 393. [Abstract] [Full Text] [PDF]

				I. Lefrere, P. de Coppet, J.-C. Camelin, S. Le Lay, N. Mercier, N. Elshourbagy, A. Bril, I. Berrebi-Bertrand, B. Feve, and S. Krief Neuropeptide AF and FF Modulation of Adipocyte Metabolism. PRIMARY INSIGHTS FROM FUNCTIONAL GENOMICS AND EFFECTS ON beta -ADRENERGIC RESPONSIVENESS J. Biol. Chem., October 11, 2002; 277(42): 39169 - 39178. [Abstract] [Full Text] [PDF]

				J. Mei, L. S. Holst, T. R. Landstrom, C. Holm, D. Brindley, V. Manganiello, and E. Degerman C2-Ceramide Influences the Expression and Insulin-Mediated Regulation of Cyclic Nucleotide Phosphodiesterase 3B and Lipolysis in 3T3-L1 Adipocytes Diabetes, March 1, 2002; 51(3): 631 - 637. [Abstract] [Full Text] [PDF]

				J. Zhou, M. Xia Shi, T. D. Mitchell, G. N. Smagin, S. R. Thomas, D. H. Ryan, and R. B.S. Harris Changes in Rat Adipocyte and Liver Glucose Metabolism Following Repeated Restraint Stress Experimental Biology and Medicine, April 1, 2001; 226(4): 312 - 319. [Abstract] [Full Text]

				S. Schaak, C. Cayla, A. Lymperopoulos, C. Flordellis, D. Cussac, C. Denis, and H. Paris Transcriptional Down-Regulation of the Human alpha 2C-Adrenergic Receptor by cAMP Mol. Pharmacol., October 1, 2000; 58(4): 821 - 827. [Abstract] [Full Text]

				F. Boussouar, R. Grataroli, J. Ji, and M. Benahmed Tumor Necrosis Factor-{alpha} Stimulates Lactate Dehydrogenase A Expression in Porcine Cultured Sertoli Cells: Mechanisms of Action Endocrinology, July 1, 1999; 140(7): 3054 - 3062. [Abstract] [Full Text]