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Volume 272, Number 39, Issue of September 26, 1997 pp. 24710-24716
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Restoration of Cell-to-Cell Communication in Thyroid Cell Lines by Transfection with and Stable Expression of the Connexin-32 Gene
IMPACT ON CELL PROLIFERATION AND TISSUE-SPECIFIC GENE EXPRESSION

(Received for publication, April 29, 1997)

From INSERM U369, Faculté de Médecine Lyon-RTH Laënnec, rue G. Paradin, 69372 Lyon Cedex 08, France

Normal thyroid epithelial cells coexpress connexin-32 and connexin-43, which form distinct gap junctions. In primary culture, connexin-43 is expressed by thyrocytes in monolayers or reorganized into follicles, whereas the expression of connexin-32 is dependent upon the reconstitution of follicles. To study the functional impact of connexin-32 gap junctions in thyroid cells, we transfected connexin-32 cDNA in two thyroid-derived communication-deficient cell lines, FRT and FRTL-5. The selected clones, which stably expressed connexin-32 at high levels and exhibited high gap junction-mediated dye-coupling, presented a reduced proliferation rate as compared with that of the corresponding wild-type FRT and FRTL-5 cells; the mean population doubling time was increased by ~35%. The proliferation of connexin-32-transfected FRTL-5 cells remained thyrotropin-dependent; the range of thyrotropin concentrations that stimulated growth was the same in transfected and control cells. The expression of connexin-32 led to an increase of thyroglobulin gene expression in FRTL-5 cells. The expression of two other tissue-specific proteins, thyroid transcription factor-1 and Pax-8, was unchanged. These findings provide evidence that connexin-32 gap junction-mediated cell-to-cell communication participates in the control of growth and differentiation of thyroid cells.

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