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(Received for publication, April 29, 1997)
From INSERM U369, Faculté de Médecine Lyon-RTH
Laënnec, rue G. Paradin, 69372 Lyon Cedex 08, France
Normal thyroid epithelial cells coexpress
connexin-32 and connexin-43, which form distinct gap junctions. In
primary culture, connexin-43 is expressed by thyrocytes in monolayers
or reorganized into follicles, whereas the expression of connexin-32 is
dependent upon the reconstitution of follicles. To study the functional impact of connexin-32 gap junctions in thyroid cells, we transfected connexin-32 cDNA in two thyroid-derived communication-deficient cell lines, FRT and FRTL-5. The selected clones, which stably expressed
connexin-32 at high levels and exhibited high gap junction-mediated dye-coupling, presented a reduced proliferation rate as compared with
that of the corresponding wild-type FRT and FRTL-5 cells; the mean
population doubling time was increased by ~35%. The proliferation of
connexin-32-transfected FRTL-5 cells remained
thyrotropin-dependent; the range of thyrotropin
concentrations that stimulated growth was the same in transfected and
control cells. The expression of connexin-32 led to an increase of
thyroglobulin gene expression in FRTL-5 cells. The expression of two
other tissue-specific proteins, thyroid transcription factor-1 and
Pax-8, was unchanged. These findings provide evidence that connexin-32
gap junction-mediated cell-to-cell communication participates in the
control of growth and differentiation of thyroid cells.
Volume 272, Number 39,
Issue of September 26, 1997
pp. 24710-24716
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
IMPACT ON CELL PROLIFERATION AND TISSUE-SPECIFIC GENE
EXPRESSION
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