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(Received for publication, October 2, 1996, and in revised form, November 28, 1996)
From the Department of Biochemistry, Weizmann Institute of Science,
Rehovot 76100, Israel
In mammalian cells, many secretory proteins are
targeted to the endoplasmic reticulum co-translationally, by the signal
recognition particle (SRP) and its receptor. In Escherichia
coli, the targeting of secretory proteins to the inner membrane
can be accomplished post-translationally. Unexpectedly, despite this
variance, E. coli contains essential genes encoding Ffh and
FtsY with a significant similarity to proteins of the eukaryotic SRP
machinery. In this study, we investigated the possibility that the
prokaryotic SRP-like machinery is involved in biogenesis of membrane
proteins in E. coli. The data presented here demonstrate
that the SRP-receptor homologue, FtsY, is indeed essential for
expression of integral membrane proteins in E. coli,
indicating that, in the case of this group of proteins, FtsY and the
mammalian SRP receptor have similar functions.
Volume 272, Number 4,
Issue of January 24, 1997
pp. 2053-2055
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
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