|
|
|
|||||||
|
|||||||||
(Received for publication, August 27, 1996, and in revised form, October 24, 1996)
From the Department of Medicine, Division of Endocrinology and
Metabolism, Dartmouth Medical School,
Lebanon, New Hampshire 03756
"Spot 14" protein appears rapidly in nuclei
of hepatocytes exposed to glucose and thyroid hormone. Exposure of
glucose- and T3-treated hepatocytes to a spot 14 antisense
oligonucleotide inhibited induction of mRNAs encoding malic enzyme,
ATP citrate-lyase, fatty acid synthase, liver-type pyruvate kinase,
phosphoenolpyruvate carboxykinase, and type I deiodinase but not
hydroxymethylglutaryl-CoA reductase, cytochrome c, and
actin mRNAs. Induction of spot 14, ATP citrate-lyase, and fatty
acid synthase polypeptides, but not propionyl-CoA carboxylase and
mitochondrial pyruvate carboxylase, was inhibited. Antisense treatment
of hepatocytes transfected with a reporter controlled by a glucose- and
T3-inducible fragment of the pyruvate kinase gene promoter
inhibited reporter activity, as did cotransfection of the reporter and
a spot 14 antisense plasmid. Spot 14 protein acts in the induction of
mRNAs coding for key lipogenic (malic enzyme, ATP citrate-lyase,
fatty acid synthase), glycolytic (pyruvate kinase), and gluconeogenic
enzymes (phosphoenolpyruvate carboxykinase), as well as the
diet-responsive type I deiodinase, but not those involved in
mitochondrial respiration (cytochrome c) or cholesterol
synthesis (hydroxymethylglutaryl-CoA reductase). Transfection
experiments indicated that these effects are mediated at the
transcriptional level. The protein functions in the activation of genes
involved in metabolic switching between the fasted and fed states in
liver.
Volume 272, Number 4,
Issue of January 24, 1997
pp. 2163-2166
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Hashimoto, E. Ishida, S. Matsumoto, S. Okada, M. Yamada, T. Satoh, T. Monden, and M. Mori Carbohydrate Response Element Binding Protein Gene Expression Is Positively Regulated by Thyroid Hormone Endocrinology, July 1, 2009; 150(7): 3417 - 3424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Schliebe, R. Strotmann, K. Busse, D. Mitschke, H. Biebermann, L. Schomburg, J. Kohrle, J. Bar, H. Rompler, J. Wess, et al. V2 vasopressin receptor deficiency causes changes in expression and function of renal and hypothalamic components involved in electrolyte and water homeostasis Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1177 - F1190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Perra, G. Simbula, M. Simbula, M. Pibiri, M. A. Kowalik, P. Sulas, M. T. Cocco, G. M. Ledda-Columbano, and A. Columbano Thyroid hormone (T3) and TR{beta} agonist GC-1 inhibit/reverse nonalcoholic fatty liver in rats FASEB J, August 1, 2008; 22(8): 2981 - 2989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. P. Cao, S. Z. Wang, Q. G. Wang, Y. X. Wang, and H. Li Association of Spot14{alpha} Gene Polymorphisms with Body Weight in the Chicken Poult. Sci., September 1, 2007; 86(9): 1873 - 1880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ishihara, E. Matsumoto, K. Horikawa, T. Kudo, E. Sakao, A. Nemoto, K. Iwase, H. Sugiyama, Y. Tamura, S. Shibata, et al. Multifactorial Regulation of Daily Rhythms in Expression of the Metabolically Responsive Gene Spot14 in the Mouse Liver J Biol Rhythms, August 1, 2007; 22(4): 324 - 334. [Abstract] [PDF]
|
|
|
|
|
|
Y.-Y. Liu, R. S. Heymann, F. Moatamed, J. J. Schultz, D. Sobel, and G. A. Brent A Mutant Thyroid Hormone Receptor {alpha} Antagonizes Peroxisome Proliferator-Activated Receptor {alpha} Signaling in Vivo and Impairs Fatty Acid Oxidation Endocrinology, March 1, 2007; 148(3): 1206 - 1217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Harvatine and D. E. Bauman SREBP1 and Thyroid Hormone Responsive Spot 14 (S14) Are Involved in the Regulation of Bovine Mammary Lipid Synthesis during Diet-Induced Milk Fat Depression and Treatment with CLA J. Nutr., October 1, 2006; 136(10): 2468 - 2474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. B. Kinlaw, J. L. Quinn, W. A. Wells, C. Roser-Jones, and J. T. Moncur Spot 14: A Marker of Aggressive Breast Cancer and a Potential Therapeutic Target Endocrinology, September 1, 2006; 147(9): 4048 - 4055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. T. LaFave, L. B. Augustin, and C. N. Mariash S14: Insights from Knockout Mice Endocrinology, September 1, 2006; 147(9): 4044 - 4047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lupu and J. A. Menendez Targeting Fatty Acid Synthase in Breast and Endometrial Cancer: An Alternative to Selective Estrogen Receptor Modulators? Endocrinology, September 1, 2006; 147(9): 4056 - 4066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Siculella, S. Sabetta, A. M. Giudetti, and G. V. Gnoni Hypothyroidism Reduces Tricarboxylate Carrier Activity and Expression in Rat Liver Mitochondria by Reducing Nuclear Transcription Rate and Splicing Efficiency J. Biol. Chem., July 14, 2006; 281(28): 19072 - 19080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhu, G. W. Anderson, G. T. Mucha, E. J. Parks, J. K. Metkowski, and C. N. Mariash The Spot 14 Protein Is Required for de Novo Lipid Synthesis in the Lactating Mammary Gland Endocrinology, August 1, 2005; 146(8): 3343 - 3350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Silander, L. J. Scott, T. T. Valle, K. L. Mohlke, H. M. Stringham, K. R. Wiles, W. L. Duren, K. F. Doheny, E. W. Pugh, P. Chines, et al. A Large Set of Finnish Affected Sibling Pair Families With Type 2 Diabetes Suggests Susceptibility Loci on Chromosomes 6, 11, and 14 Diabetes, March 1, 2004; 53(3): 821 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Campbell, G. W. Anderson, and C. N. Mariash Human Spot 14 Glucose and Thyroid Hormone Response: Characterization and Thyroid Hormone Response Element Identification Endocrinology, December 1, 2003; 144(12): 5242 - 5248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sakao, A. Ishihara, K. Horikawa, M. Akiyama, M. Arai, M. Kato, N. Seki, K. Fukunaga, A. Shimizu-Yabe, K. Iwase, et al. Two-peaked Synchronization in Day/Night Expression Rhythms of the Fibrinogen Gene Cluster in the Mouse Liver J. Biol. Chem., August 15, 2003; 278(33): 30450 - 30457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, L. Yin, and F. B. Hillgartner SREBP-1 integrates the actions of thyroid hormone, insulin, cAMP, and medium-chain fatty acids on ACC{alpha} transcription in hepatocytes J. Lipid Res., February 1, 2003; 44(2): 356 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cachefo, P. Boucher, C. Vidon, E. Dusserre, F. Diraison, and M. Beylot Hepatic Lipogenesis and Cholesterol Synthesis in Hyperthyroid Patients J. Clin. Endocrinol. Metab., November 1, 2001; 86(11): 5353 - 5357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Feng, Y. Jiang, P. Meltzer, and P. M. Yen Thyroid Hormone Regulation of Hepatic Genes in Vivo Detected by Complementary DNA Microarray Mol. Endocrinol., July 1, 2000; 14(7): 947 - 955. [Abstract] [Full Text]
|
|
|
|
|
|
S.-H. Koo and H. C. Towle Glucose Regulation of Mouse S14 Gene Expression in Hepatocytes. INVOLVEMENT OF A NOVEL TRANSCRIPTION FACTOR COMPLEX J. Biol. Chem., February 18, 2000; 275(7): 5200 - 5207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Moncur, J. P. Park, V. A. Memoli, T. K. Mohandas, and W. B. Kinlaw The "Spot 14" gene resides on the telomeric end of the 11q13 amplicon and is expressed in lipogenic breast cancers: Implications for control of tumor metabolism PNAS, June 9, 1998; 95(12): 6989 - 6994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Cunningham, M. Maloney, and W. B. Kinlaw Spot 14 Protein-Protein Interactions: Evidence for Both Homo- and Heterodimer Formation in Vivo Endocrinology, December 1, 1997; 138(12): 5184 - 5188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yu and R. J. Koenig Regulation of Hepatocyte Thyroxine 5'-Deiodinase by T3 and Nuclear Receptor Coactivators as a Model of the Sick Euthyroid Syndrome J. Biol. Chem., December 1, 2000; 275(49): 38296 - 38301. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |