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(Received for publication, August 30, 1996)
From the Human Retrovirus Section, ABL-Basic Research Program,
NCI-FCRDC, Frederick, Maryland 21702-1201
Human immunodeficiency virus type 1 (HIV-1)
mRNAs encoding structural proteins contain multiple
inhibitory/instability elements (INS), which decrease the efficiency of
viral protein expression. We have previously identified a strong INS
element (INS-1) within the p17gag coding region. Here we show
that poly(A)-binding protein 1 (PABP1) binds preferentially to INS-1
within the p17gag mRNA, but not to a mutated mRNA in
which INS-1 function is eliminated. Competition experiments performed
in the presence of different nucleic acids and homoribopolymers
demonstrated preferential binding of PABP1 to the INS-1-containing RNA.
In contrast to HeLa cells and several lymphoid cell lines, certain
human glioma cell lines exhibit high levels of gag
expression in the absence of Rev upon transient transfection with wild
type gag expression vectors. We analyzed extracts of
different cell lines and found that the binding of PABP1 to INS-1 RNA
is significantly diminished in glial cell extracts. The expression
levels of gag correlate with the absence of binding of
PABP1 to the INS-1 RNA in cellular extracts. These results suggest a
role for PABP1 in the inhibition of gag expression mediated
through INS-1.
Volume 272, Number 4,
Issue of January 24, 1997
pp. 2307-2311
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
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