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Volume 272, Number 40, Issue of October 3, 1997 pp. 25333-25338
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Modulation of Cardiac Ryanodine Receptors by Sorcin

(Received for publication, March 31, 1997, and in revised form, July 3, 1997)

Andrew J. Lokuta Dagger , Marian B. Meyers § , Paul R. Sander Dagger , Glenn I. Fishman § and Hector H. Valdivia Dagger

From the Dagger  Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin 53706, and the § Department of Medicine, Section of Molecular Cardiology, Albert Einstein College of Medicine, Bronx, New York 10461

Sorcin is a widely expressed, 22-kDa Ca2+-binding protein initially identified in multidrug-resistant cells. In the heart, sorcin localizes to the dyadic junctions of transverse tubules and sarcoplasmic reticulum and coimmunoprecipitates with the Ca2+ release channel/ryanodine receptor (RyR) (Meyers, M. B., Pickel, V. M., Sheu, S.-S., Sharma, V. K., Scotto, K. W., and Fishman, G. I. (1995) J. Biol. Chem. 270, 26411-26418). We have investigated a possible functional interaction between sorcin and cardiac RyR using purified recombinant sorcin in [3H]ryanodine binding experiments and single channel recordings of RyR. The open probability of single RyR was decreased significantly by the addition of sorcin to the cytoplasmic side of the channel (IC50 ~ 480 nM). In addition, sorcin completely inhibited [3H]ryanodine binding with an IC50 ~ 700 nM. Inhibition occurred over a wide range of [Ca2+], and sorcin-modulated RyR remained Ca2+-dependent. Furthermore, caffeine-activated RyRs were also inhibited by sorcin at low [Ca2+] (pCa 7), suggesting that Ca2+ is not an obligatory factor for sorcin inhibition of RyR. Comparisons of these inhibitory effects with those of calmodulin and calpain, proteins structurally related to sorcin, suggested that the interaction of sorcin with cardiac RyR was distinct from and independent of either of these modulatory proteins. Phosphorylation of sorcin with the catalytic subunit of protein kinase A significantly decreased the ability of sorcin to modulate RyR. These results suggest that sorcin may modulate RyR function in a normal cell environment and that the level of modulation is in turn influenced by signaling pathways that increase protein kinase A activity.


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