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Volume 272, Number 41, Issue of October 10, 1997 pp. 25768-25777
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Mts4, a Non-ATPase Subunit of the 26 S Protease in Fission Yeast Is Essential for Mitosis and Interacts Directly with the ATPase Subunit Mts2

(Received for publication, January 30, 1997, and in revised form, June 23, 1997)

Caroline R. M. Wilkinson , Mairi Wallace , Michael Seeger ^¶ , Wolfgang Dubiel ^¶ and Colin Gordon

From the  MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU Scotland, United Kingdom and the ^¶ Institute of Biochemistry, Medical Faculty, Humboldt University, Monbijoustrasse 2A 10117, Berlin, Federal Republic of Germany

We have isolated a fission yeast gene, mts4⁺, by complementation of a temperature-sensitive mutation and show that it encodes subunit 2 (S2) of the 19 S regulatory complex of the 26 S protease. mts4⁺ is an essential gene, and we show that loss of this subunit causes cells to arrest in metaphase, illustrating the importance of S2 for mitosis. The Mts4 protein is 48% identical to S2 of the human 26 S protease, and the lethal phenotype of the null mts4 allele can be rescued by the human cDNA encoding S2. We provide genetic and physical evidence to suggest that the Mts4 protein interacts with the product of the mts2⁺ gene, an ATPase which has previously been shown to be subunit 4 of the 26 S protease.

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