JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Davis, D. A.
Right arrow Articles by Yarchoan, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Davis, D. A.
Right arrow Articles by Yarchoan, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Volume 272, Number 41, Issue of October 10, 1997 pp. 25935-25940
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Thioltransferase (Glutaredoxin) Is Detected Within HIV-1 and Can Regulate the Activity of Glutathionylated HIV-1 Protease in Vitro

(Received for publication, June 4, 1997, and in revised form, August 7, 1997)

David A. Davis , Fonda M. Newcomb , David W. Starke § , David E. Ott , John J. Mieyal § and Robert Yarchoan

From the HIV and AIDS Malignancy Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892, the § Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, and the  AIDS Vaccine Program, Science Applications International Corporation Frederick, Frederick Cancer Research and Development Center, Frederick, Maryland 21702

Previous studies have suggested that the two conserved cysteines of the HIV-1 protease may be involved in regulating protease activity. Here, we examined diglutathionylated wild type protease (Cys-67-SSG, Cys-95-SSG) and the monoglutathionylated protease mutants (C67A, Cys-95-SSG and C95A, Cys-67-SSG) as potential substrates for thioltransferase (glutaredoxin). Time-dependent changes in the extent of deglutathionylation of each protein were assayed by reverse phase-high performance liquid chromatography. Glutathione alone was not an effective reductant, whereas thioltransferase displayed differential catalysis toward the Cys-95-SSG and Cys-67-SSG sites. At low thioltransferase concentrations (5 nM), deglutathionylation occurred almost exclusively at Cys-95-SSG. With substantially more thioltransferase (100 nM) Cys-67-SSG was partially deglutathionylated but only at 20% of the rate of Cys-95-SSG reduction. Treatment of the diglutathionylated protease with thioltransferase not only restored protease activity but generated an enzyme preparation that had a 3- to 5-fold greater specific activity relative to the fully reduced form. Immunoblot analysis of HIV-1MN virus with an antibody to thioltransferase detected a band co-migrating with recombinant thioltransferase that persisted following subtilisin treatment, indicating the presence of thioltransferase within HIV-1. Our results implicate thioltransferase in the regulation and/or maintenance of protease activity in HIV-1 infected cells.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. Melchers, N. Dirdjaja, T. Ruppert, and R. L. Krauth-Siegel
Glutathionylation of Trypanosomal Thiol Redox Proteins
J. Biol. Chem., March 23, 2007; 282(12): 8678 - 8694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Berndt, C. H. Lillig, and A. Holmgren
Thiol-based mechanisms of the thioredoxin and glutaredoxin systems: implications for diseases in the cardiovascular system
Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1227 - H1236.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Kanda, Y. Ihara, H. Murata, Y. Urata, T. Kono, J. Yodoi, S. Seto, K. Yano, and T. Kondo
Glutaredoxin Modulates Platelet-derived Growth Factor-dependent Cell Signaling by Regulating the Redox Status of Low Molecular Weight Protein-tyrosine Phosphatase
J. Biol. Chem., September 29, 2006; 281(39): 28518 - 28528.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. B. Park and M. Levine
Cloning, Sequencing, and Characterization of Alternatively Spliced Glutaredoxin 1 cDNA and Its Genomic Gene: CHROMOSOMAL LOCALIZATION, mRNA STABILITY, AND ORIGIN OF PSEUDOGENES
J. Biol. Chem., March 18, 2005; 280(11): 10427 - 10434.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. H. Lillig, M. E. Lonn, M. Enoksson, A. P. Fernandes, and A. Holmgren
Short interfering RNA-mediated silencing of glutaredoxin 2 increases the sensitivity of HeLa cells toward doxorubicin and phenylarsine oxide
PNAS, September 7, 2004; 101(36): 13227 - 13232.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Ortega, P. Ferrer, J. Carretero, E. Obrador, M. Asensi, J. A. Pellicer, and J. M. Estrela
Down-regulation of Glutathione and Bcl-2 Synthesis in Mouse B16 Melanoma Cells Avoids Their Survival during Interaction with the Vascular Endothelium
J. Biol. Chem., October 10, 2003; 278(41): 39591 - 39599.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Cartier, B. Hemonnot, B. Gay, M. Bardy, C. Sanchiz, C. Devaux, and L. Briant
Active cAMP-dependent Protein Kinase Incorporated within Highly Purified HIV-1 Particles Is Required for Viral Infectivity and Interacts with Viral Capsid Protein
J. Biol. Chem., September 12, 2003; 278(37): 35211 - 35219.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. A. Davis, C. A. Brown, F. M. Newcomb, E. S. Boja, H. M. Fales, J. Kaufman, S. J. Stahl, P. Wingfield, and R. Yarchoan
Reversible Oxidative Modification as a Mechanism for Regulating Retroviral Protease Dimerization and Activation
J. Virol., March 1, 2003; 77(5): 3319 - 3325.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
N. ROUHIER, E. GELHAYE, and J.-P. JACQUOT
Redox Control by Dithiol-Disulfide Exchange in Plants: II. The Cytosolic and Mitochondrial Systems
Ann. N.Y. Acad. Sci., November 1, 2002; 973(1): 520 - 528.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Furuhata, M. Nakamura, T. Osawa, and K. Uchida
Thiolation of Protein-bound Carcinogenic Aldehyde. AN ELECTROPHILIC ACROLEIN-LYSINE ADDUCT THAT COVALENTLY BINDS TO THIOLS
J. Biol. Chem., July 26, 2002; 277(31): 27919 - 27926.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. J. Collinson, G. L. Wheeler, E. O. Garrido, A. M. Avery, S. V. Avery, and C. M. Grant
The Yeast Glutaredoxins Are Active as Glutathione Peroxidases
J. Biol. Chem., May 3, 2002; 277(19): 16712 - 16717.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Shenton, G. Perrone, K. A. Quinn, I. W. Dawes, and C. M. Grant
Regulation of Protein S-Thiolation by Glutaredoxin 5 in the Yeast Saccharomyces cerevisiae
J. Biol. Chem., May 3, 2002; 277(19): 16853 - 16859.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
D. A. Davis, E. Read-Connole, K. Pearson, H. M. Fales, F. M. Newcomb, J. Moskovitz, and R. Yarchoan
Oxidative Modifications of Kynostatin-272, a Potent Human Immunodeficiency Virus Type 1 Protease Inhibitor: Potential Mechanism for Altered Activity in Monocytes/Macrophages
Antimicrob. Agents Chemother., February 1, 2002; 46(2): 402 - 408.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. D. Parker and E. Hunter
Activation of the Mason-Pfizer monkey virus protease within immature capsids invitro
PNAS, November 20, 2001; (2001) 251460998.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
L. Sahlin, H. Wang, Y. Stjernholm, M. Lundberg, G. Ekman, A. Holmgren, and H. Eriksson
The expression of glutaredoxin is increased in the human cervix in term pregnancy and immediately post-partum, particularly after prostaglandin-induced delivery
Mol. Hum. Reprod., December 1, 2000; 6(12): 1147 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
L. Garcia-Pardo, M.D. Granados, F. Gaytan, C.A. Padilla, E. Martinez-Galisteo, C. Morales, J.E. Sanchez-Criado, and J.A. Barcena
Immunolocalization of glutaredoxin in the human corpus luteum
Mol. Hum. Reprod., October 1, 1999; 5(10): 914 - 919.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
P. KLATT, E. P. MOLINA, M. G. DE LACOBA, C. A. PADILLA, E. MARTÍNEZ-GALISTEO, J. A. BÁRCENA, and S. LAMAS
Redox regulation of c-Jun DNA binding by reversible S-glutathiolation
FASEB J, September 1, 1999; 13(12): 1481 - 1490.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
C. M. Grant, K. A. Quinn, and I. W. Dawes
Differential Protein S-Thiolation of Glyceraldehyde-3-Phosphate Dehydrogenase Isoenzymes Influences Sensitivity to Oxidative Stress
Mol. Cell. Biol., April 1, 1999; 19(4): 2650 - 2656.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. A. Davis, K. Yusa, L. A. Gillim, F. M. Newcomb, H. Mitsuya, and R. Yarchoan
Conserved Cysteines of the Human Immunodeficiency Virus Type 1 Protease Are Involved in Regulation of Polyprotein Processing and Viral Maturation of Immature Virions
J. Virol., February 1, 1999; 73(2): 1156 - 1164.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Bandyopadhyay, D. W. Starke, J. J. Mieyal, and R. M. Gronostajski
Thioltransferase (Glutaredoxin) Reactivates the DNA-binding Activity of Oxidation-inactivated Nuclear Factor I
J. Biol. Chem., January 2, 1998; 273(1): 392 - 397.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. A. Chrestensen, D. W. Starke, and J. J. Mieyal
Acute Cadmium Exposure Inactivates Thioltransferase (Glutaredoxin), Inhibits Intracellular Reduction of Protein-glutathionyl-mixed Disulfides, and Initiates Apoptosis
J. Biol. Chem., August 18, 2000; 275(34): 26556 - 26565.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Rahlfs, M. Fischer, and K. Becker
Plasmodium falciparum Possesses a Classical Glutaredoxin and a Second, Glutaredoxin-like Protein with a PICOT Homology Domain
J. Biol. Chem., September 28, 2001; 276(40): 37133 - 37140.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. D. Parker and E. Hunter
Activation of the Mason-Pfizer monkey virus protease within immature capsids invitro
PNAS, December 4, 2001; 98(25): 14631 - 14636.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.