HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Han, J.-D. Articles by Rubin, C. S. Search for Related Content PubMed PubMed Citation Articles by Han, J.-D. Articles by Rubin, C. S. Social Bookmarking                      What's this?

Volume 272, Number 42, Issue of October 17, 1997 pp. 26611-26619
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Molecular Characterization of a Novel A Kinase Anchor Protein from Drosophila melanogaster

(Received for publication, May 16, 1997, and in revised form, July 16, 1997)

Jing-Dong Han , Nicholas E. Baker ^§ and Charles S. Rubin

From the  Department of Molecular Pharmacology, Atran Laboratories and the ^§ Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461

Activation of protein kinase A (PKA) at discrete intracellular sites facilitates oogenesis and development in Drosophila. Thus, PKA-anchor protein complexes may be involved in controlling these crucial biological processes. Evaluation of this proposition requires knowledge of PKA binding/targeting proteins in the fly. We now report the discovery and characterization of cDNAs encoding a novel, Drosophila A kinase anchor protein, DAKAP550. DAKAP550 is a large (>2300 amino acids) acidic protein that is maximally expressed in anterior tissues. It binds regulatory subunits (RII) of both mammalian and Drosophila PKAII isoforms. The tethering region of DAKAP550 includes two proximal, but non-contiguous RII-binding sites (B1 and B2). The B1 domain (residues 1406-1425) binds RII ~20-fold more avidly than B2 (amino acids 1350-1369). Affinity-purified anti-DAKAP550 IgGs were exploited to demonstrate that the anchor protein is expressed in many cells in nearly all tissues throughout the lifespan of the fly. However, DAKAP550 is highly enriched and asymmetrically positioned in subpopulations of neurons and in apical portions of cells in gut and trachea. The combination of RII (PKAII) binding activity with differential expression and polarized localization is consistent with a role for DAKAP550 in creating target loci for the reception of signals carried by cAMP. The DAKAP550 gene was mapped to the 4F1.2 region of the X chromosome; flies that carry a deletion for this portion of the X chromosome lack DAKAP550 protein.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. S. Goehring, B. S. Pedroja, S. A. Hinke, L. K. Langeberg, and J. D. Scott MyRIP Anchors Protein Kinase A to the Exocyst Complex J. Biol. Chem., November 9, 2007; 282(45): 33155 - 33167. [Abstract] [Full Text] [PDF]

				Y. Lu, Y.-S. Lu, Y. Shuai, C. Feng, T. Tully, Z. Xie, Y. Zhong, and H.-M. Zhou The AKAP Yu is required for olfactory long-term memory formation in Drosophila PNAS, August 21, 2007; 104(34): 13792 - 13797. [Abstract] [Full Text] [PDF]

				N. de Souza, L. G. Vallier, H. Fares, and I. Greenwald SEL-2, the C. elegans neurobeachin/LRBA homolog, is a negative regulator of lin-12/Notch activity and affects endosomal traffic in polarized epithelial cells Development, February 15, 2007; 134(4): 691 - 702. [Abstract] [Full Text] [PDF]

				Y. Su, R. J. Balice-Gordon, D. M. Hess, D. S. Landsman, J. Minarcik, J. Golden, I. Hurwitz, S. A. Liebhaber, and N. E. Cooke Neurobeachin Is Essential for Neuromuscular Synaptic Transmission J. Neurosci., April 7, 2004; 24(14): 3627 - 3636. [Abstract] [Full Text] [PDF]

				K. TASKEN and E. M. AANDAHL Localized Effects of cAMP Mediated by Distinct Routes of Protein Kinase A Physiol Rev, January 1, 2004; 84(1): 137 - 167. [Abstract] [Full Text] [PDF]

				S. J. Khundmiri, M. J. Rane, and E. D. Lederer Parathyroid Hormone Regulation of Type II Sodium-Phosphate Cotransporters Is Dependent on an A Kinase Anchoring Protein J. Biol. Chem., March 14, 2003; 278(12): 10134 - 10141. [Abstract] [Full Text] [PDF]

				H. Li, R. Adamik, G. Pacheco-Rodriguez, J. Moss, and M. Vaughan Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) PNAS, February 18, 2003; 100(4): 1627 - 1632. [Abstract] [Full Text] [PDF]

				H. K. Shamloula, M. P. Mbogho, A. C. Pimentel, Z. M. A. Chrzanowska-Lightowlers, V. Hyatt, H. Okano, and T. R. Venkatesh rugose (rg), a Drosophila A kinase Anchor Protein, Is Required for Retinal Pattern Formation and Interacts Genetically With Multiple Signaling Pathways Genetics, June 1, 2002; 161(2): 693 - 710. [Abstract] [Full Text] [PDF]

				S. M. Jackson and C. A. Berg An A-kinase anchoring protein is required for Protein kinase A regulatory subunit localization and morphology of actin structures during oogenesis in Drosophila Development, January 10, 2002; 129(19): 4423 - 4433. [Abstract] [Full Text] [PDF]

				J. A. Kiger Jr. and C. O'Shea Genetic Evidence for a Protein Kinase A/Cubitus Interruptus Complex That Facilitates Processing of Cubitus Interruptus in Drosophila Genetics, July 1, 2001; 158(3): 1157 - 1166. [Abstract] [Full Text] [PDF]

				J.-W. Wang, J. Howson, E. Haller, and W. G. Kerr Identification of a Novel Lipopolysaccharide-Inducible Gene with Key Features of Both a Kinase Anchor Proteins and chs1/beige Proteins J. Immunol., April 1, 2001; 166(7): 4586 - 4595. [Abstract] [Full Text] [PDF]

				X. Wang, F. W. Herberg, M. M. Laue, C. Wullner, B. Hu, E. Petrasch-Parwez, and M. W. Kilimann Neurobeachin: A Protein Kinase A-Anchoring, beige/Chediak-Higashi Protein Homolog Implicated in Neuronal Membrane Traffic J. Neurosci., December 1, 2000; 20(23): 8551 - 8565. [Abstract] [Full Text] [PDF]

				R. G. Angelo and C. S. Rubin Characterization of Structural Features That Mediate the Tethering of Caenorhabditis elegans Protein Kinase A to a Novel A Kinase Anchor Protein. INSIGHTS INTO THE ANCHORING OF PKAI ISOFORMS J. Biol. Chem., February 11, 2000; 275(6): 4351 - 4362. [Abstract] [Full Text] [PDF]

				Z. Li, E. A. Rossi, J. D. Hoheisel, D. Kalderon, and C. S. Rubin Generation of a Novel A Kinase Anchor Protein and a Myristoylated Alanine-rich C Kinase Substrate-like Analog from a Single Gene J. Biol. Chem., September 17, 1999; 274(38): 27191 - 27200. [Abstract] [Full Text] [PDF]

				R. M. O. Turner, L. R. Johnson, L. Haig-Ladewig, G. L. Gerton, and S. B. Moss An X-linked Gene Encodes a Major Human Sperm Fibrous Sheath Protein, hAKAP82. GENOMIC ORGANIZATION, PROTEIN KINASE A-RII BINDING, AND DISTRIBUTION OF THE PRECURSOR IN THE SPERM TAIL J. Biol. Chem., November 27, 1998; 273(48): 32135 - 32141. [Abstract] [Full Text] [PDF]

				R. Angelo and C. S. Rubin Molecular Characterization of an Anchor Protein (AKAPCE) That Binds the RI Subunit (RCE) of Type I Protein Kinase A from Caenorhabditis elegans J. Biol. Chem., June 5, 1998; 273(23): 14633 - 14643. [Abstract] [Full Text] [PDF]