| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, August 21, 1997, and in revised form, September 5, 1997)
,
and
From the § Department of Biochemistry and Molecular
Biology, Mayo Foundation, Rochester, Minnesota 55905 and the
Phosphorylation by protein kinase C of the
"a" and "b" variants of plasma membrane Ca2+
pump isoforms 2 and 3 was studied. Full-length versions of these isoforms were assembled and expressed in COS cells. Whereas the "a"
forms were phosphorylated easily with PKC, isoform 2b was phosphorylated only a little, and isoform 3b was not phosphorylated at
all. Phosphorylation of isoforms 2a and 3a did not affect their basal
activity, but prevented the stimulation of their activity by calmodulin
and their binding to calmodulin-Sepharose. This indicated that
phosphorylation prevented activation of these isoforms by preventing
calmodulin binding. Based on these results, phosphorylation of the pump
with PKC would be expected to increase free intracellular Ca2+ levels in those cells where isoforms 2a and 3a are
expressed.
National Institute of Haematology and Immunology, Daroczi
ut 24, 1113 Budapest, Hungary
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. M. RUKNUDIN and E. G. LAKATTA The Regulation of the Na/Ca Exchanger and Plasmalemmal Ca2+ ATPase by Other Proteins Ann. N.Y. Acad. Sci., March 1, 2007; 1099(1): 86 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. STREHLER, A. J. CARIDE, A. G. FILOTEO, Y. XIONG, J. T. PENNISTON, and A. ENYEDI Plasma Membrane Ca2+ ATPases as Dynamic Regulators of Cellular Calcium Handling Ann. N.Y. Acad. Sci., March 1, 2007; 1099(1): 226 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Jensen, A. G. Filoteo, T. Knopfel, and R. M. Empson Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic transmission in rat hippocampal CA3 J. Physiol., February 15, 2007; 579(1): 85 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Hill, D. E. Williams, M. LeMasurier, R. A. Dumont, E. E. Strehler, and P. G. Gillespie Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles. J. Neurosci., June 7, 2006; 26(23): 6172 - 6180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Crossthwaite, A. Ciruela, T. F. Rayner, and D. M. F. Cooper A Direct Interaction between the N Terminus of Adenylyl Cyclase AC8 and the Catalytic Subunit of Protein Phosphatase 2A Mol. Pharmacol., February 1, 2006; 69(2): 608 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Baekgaard, L. Luoni, M. I. De Michelis, and M. G. Palmgren The Plant Plasma Membrane Ca2+ Pump ACA8 Contains Overlapping as Well as Physically Separated Autoinhibitory and Calmodulin-binding Domains J. Biol. Chem., January 13, 2006; 281(2): 1058 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Usachev, A. J. Marsh, T. M. Johanns, M. M. Lemke, and S. A. Thayer Activation of Protein Kinase C in Sensory Neurons Accelerates Ca2+ Uptake into the Endoplasmic Reticulum J. Neurosci., January 4, 2006; 26(1): 311 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Turner, A. K. Gelasco, and J. R. Raymond Calmodulin Interacts with the Third Intracellular Loop of the Serotonin 5-Hydroxytryptamine1A Receptor at Two Distinct Sites: PUTATIVE ROLE IN RECEPTOR PHOSPHORYLATION BY PROTEIN KINASE C J. Biol. Chem., April 23, 2004; 279(17): 17027 - 17037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. De Luisi and A. M. Hofer Evidence that Ca2+ cycling by the plasma membrane Ca2+-ATPase increases the `excitability' of the extracellular Ca2+-sensing receptor J. Cell Sci., April 15, 2003; 116(8): 1527 - 1538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. GOELLNER, S. J. DEMARCO, and E. E. STREHLER Characterization of PISP, a Novel Single-PDZ Protein That Binds to All Plasma Membrane Ca2+-ATPase b-Splice Variants Ann. N.Y. Acad. Sci., April 1, 2003; 986(1): 461 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. E. Bruce, D. I. Yule, and T. J. Shuttleworth Ca2+-dependent Protein Kinase-A Modulation of the Plasma Membrane Ca2+-ATPase in Parotid Acinar Cells J. Biol. Chem., December 6, 2002; 277(50): 48172 - 48181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Paszty, A. R. Penheiter, A. K. Verma, R. Padanyi, A. G. Filoteo, J. T. Penniston, and A. Enyedi Asp1080 Upstream of the Calmodulin-binding Domain Is Critical for Autoinhibition of hPMCA4b J. Biol. Chem., September 20, 2002; 277(39): 36146 - 36151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Banan, J. Z. Fields, A. Farhadi, D. A. Talmage, L. Zhang, and A. Keshavarzian The beta 1 Isoform of Protein Kinase C Mediates the Protective Effects of Epidermal Growth Factor on the Dynamic Assembly of F-Actin Cytoskeleton and Normalization of Calcium Homeostasis in Human Colonic Cells J. Pharmacol. Exp. Ther., June 1, 2002; 301(3): 852 - 866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Paszty, A. K. Verma, R. Padanyi, A. G. Filoteo, J. T. Penniston, and A. Enyedi Plasma Membrane Ca2+ATPase Isoform 4b Is Cleaved and Activated by Caspase-3 during the Early Phase of Apoptosis J. Biol. Chem., February 22, 2002; 277(9): 6822 - 6829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Korchak, B. E. Corkey, G. C. Yaney, and L. E. Kilpatrick Negative regulation of ligand-initiated Ca2+ uptake by PKC-{beta}II in differentiated HL60 cells Am J Physiol Cell Physiol, August 1, 2001; 281(2): C514 - C523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Dumont, U. Lins, A. G. Filoteo, J. T. Penniston, B. Kachar, and P. G. Gillespie Plasma Membrane Ca2+-ATPase Isoform 2a Is the PMCA of Hair Bundles J. Neurosci., July 15, 2001; 21(14): 5066 - 5078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. Strehler and D. A. Zacharias Role of Alternative Splicing in Generating Isoform Diversity Among Plasma Membrane Calcium Pumps Physiol Rev, January 1, 2001; 81(1): 21 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Hwang, H. Sze, and J. F. Harper A calcium-dependent protein kinase can inhibit a calmodulin-stimulated Ca2+ pump (ACA2) located in the endoplasmic reticulum of Arabidopsis PNAS, May 23, 2000; 97(11): 6224 - 6229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Filoteo, A. Enyedi, A. K. Verma, N. L. Elwess, and J. T. Penniston Plasma Membrane Ca2+ Pump Isoform 3f Is Weakly Stimulated by Calmodulin J. Biol. Chem., February 11, 2000; 275(6): 4323 - 4328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Malmström, H.-E. Åkerlund, and P. Askerlund Regulatory Role of the N Terminus of the Vacuolar Calcium-ATPase in Cauliflower Plant Physiology, February 1, 2000; 122(2): 517 - 526. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. Caride, N. L. Elwess, A. K. Verma, A. G. Filoteo, A. Enyedi, Z. Bajzer, and J. T. Penniston The Rate of Activation by Calmodulin of Isoform 4 of the Plasma Membrane Ca2+ Pump Is Slow and Is Changed by Alternative Splicing J. Biol. Chem., December 3, 1999; 274(49): 35227 - 35232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Verma, K. Paszty, A. G. Filoteo, J. T. Penniston, and A. Enyedi Protein Kinase C Phosphorylates Plasma Membrane Ca2+ Pump Isoform 4a at Its Calmodulin Binding Domain J. Biol. Chem., January 1, 1999; 274(1): 527 - 531. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
