| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, April 28, 1997, and in revised form, August 13, 1997)
From the Service de Biochimie et de Génétique
Moléculaire, CEA/Saclay, F-91191 Gif-sur-Yvette Cedex, France
and § Institut für Biochemie, Genzentrum der
Ludwig-Maximilians-Universität München,
Feodor-Lynen-Strasse 25, 1377 München
To probe the complex nucleic acid binding domains
of yeast RNA polymerase II (Pol II), we have isolated in the presence
of heparin RNA molecules that selectively bind to yeast Pol II. A class
of RNA molecules was found to bind and strongly interfere with
enzyme-DNA interaction but not with RNA chain elongation. Remarkably,
one selected RNA ligand was a specific inhibitor of Saccharomyces
cerevisiae Pol II. S. cerevisiae Pol I and Pol III and Pol II from Schizosaccharomyces pombe or wheat germ
cells were not affected. Photocross-linking experiments showed that the
RNA ligand preferentially interacted with B220, the largest subunit of
Pol II and, to a lesser extent, with B150, the second largest subunit.
The selected RNA was expressed in yeast cells under the control of a
Pol III promoter. Yeast cells that expressed the anti-Pol II aptamer
grew normally. However, a cell growth defect was observed when
expressing the RNA aptamer in cells having an artificially reduced
level of Pol II.
Volume 272, Number 44,
Issue of October 31, 1997
pp. 27980-27986
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
Selective Targeting and Inhibition of Yeast RNA Polymerase II
by RNA Aptamers
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. A. Espinoza, J. A. Goodrich, and J. F. Kugel Characterization of the structure, function, and mechanism of B2 RNA, an ncRNA repressor of RNA polymerase II transcription RNA, April 1, 2007; 13(4): 583 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhao, H. Shi, A. Sevilimedu, N. Liachko, H. C. M. Nelson, and J. T. Lis An RNA aptamer that interferes with the DNA binding of the HSF transcription activator Nucleic Acids Res., August 7, 2006; 34(13): 3755 - 3761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mi, X. Zhang, Z. N Rabbani, Y. Liu, Z. Su, Z. Vujaskovic, C. D. Kontos, B. A. Sullenger, and B. M. Clary H1 RNA polymerase III promoter-driven expression of an RNA aptamer leads to high-level inhibition of intracellular protein activity Nucleic Acids Res., July 19, 2006; 34(12): 3577 - 3584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. Gening, S. A. Klincheva, A. Reshetnjak, A. P. Grollman, and H. Miller RNA aptamers selected against DNA polymerase {beta} inhibit the polymerase activities of DNA polymerases beta and kappa. Nucleic Acids Res., January 1, 2006; 34(9): 2579 - 2586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fitter and R. James Deconvolution of a Complex Target Using DNA Aptamers J. Biol. Chem., October 7, 2005; 280(40): 34193 - 34201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Saha, A. Z. Ansari, K. A. Jarrell, and M. Ptashne RNA sequences that work as transcriptional activating regions Nucleic Acids Res., March 1, 2003; 31(5): 1565 - 1570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Biroccio, J. Hamm, I. Incitti, R. De Francesco, and L. Tomei Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase J. Virol., March 19, 2002; 76(8): 3688 - 3696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Simons, N. Dafni, I. Dotan, Y. Oron, and D. Canaani Genetic synthetic lethality screen at the single gene level in cultured human cells Nucleic Acids Res., October 15, 2001; 29(20): e100 - e100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Tlsty Searching for Targets: The Power of Somatic Cell Genetics Genome Res., February 1, 2001; 11(2): 187 - 188. [Full Text]
|
|
|
|
|
|
A. Simons, N. Dafni, I. Dotan, Y. Oron, and D. Canaani Establishment of a Chemical Synthetic Lethality Screen in Cultured Human Cells Genome Res., February 1, 2001; 11(2): 266 - 273. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Shi, B. E. Hoffman, and J. T. Lis RNA aptamers as effective protein antagonists in a multicellular organism PNAS, August 31, 1999; 96(18): 10033 - 10038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Geyer, A. Colman-Lerner, and R. Brent "Mutagenesis" by peptide aptamers identifies genetic network members and pathway connections PNAS, July 20, 1999; 96(15): 8567 - 8572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Blind, W. Kolanus, and M. Famulok Cytoplasmic RNA modulators of an inside-out signal-transduction cascade PNAS, March 30, 1999; 96(7): 3606 - 3610. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
