| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, June 23, 1997, and in revised form, August 15, 1997)
From the Research Center for Cardiovascular Diseases, Institute of
Molecular Medicine for the Prevention of Human Diseases, and Division
of Molecular Medicine, Department of Internal Medicine, The University
of Texas-Houston Health Science Center, Houston, Texas 77030
Sentrin is a ubiquitin-like molecule that has
been shown to interact with the death domains of Fas and tumor necrosis
factor receptor 1 (TNFR1), PML, Rad51, Rad52, and RanGAP1. We have
reported previously that sentrin can be conjugated to other proteins in a manner analogous to protein ubiquitination (Kamitani, T., Nguyen, H. P., and Yeh, E. T. H. (1997) J. Biol.
Chem. 272, 14001-14004). Furthermore, the conserved C-terminal
Gly-Gly residues are required for sentrinization to occur. To identify
enzymes which play a role in sentrinization, the yeast two-hybrid
system was used to screen a human placenta cDNA library using
sentrin as bait. A strong positive interacting clone was found to
contain a cDNA insert encoding the ubiquitin-conjugating enzyme,
Ubc9. The interaction between sentrin and Ubc9 required the ubiquitin
domain and the C-terminal Gly-Gly residues of sentrin. This interaction
appears to be specific because sentrin could only interact weakly with UbcH5B, but could not interact with HHR6B, UbcH6 nor E2-EPF. In vitro translated sentrin could be precipitated by a GST-Ubc9
fusion protein, but not by glutathione S-transferase. A
-mercaptoethanol-sensitive Ubc9-sentrin conjugate could also be
identified in the in vitro binding assay. Substitution of
the conserved cysteine residue of Ubc9 by serine abolished the
formation of the Ubc9-sentrin conjugate. Taken together, Ubc9 is a
strong candidate to be the key conjugating enzyme in the sentrinization
pathway.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Bawa-Khalfe, J. Cheng, Z. Wang, and E. T. H. Yeh Induction of the SUMO-specific Protease 1 Transcription by the Androgen Receptor in Prostate Cancer Cells J. Biol. Chem., December 28, 2007; 282(52): 37341 - 37349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Siatecka, L. Xue, and J. J. Bieker Sumoylation of EKLF Promotes Transcriptional Repression and Is Involved in Inhibition of Megakaryopoiesis Mol. Cell. Biol., December 15, 2007; 27(24): 8547 - 8560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Shalizi, P. M. Bilimoria, J. Stegmuller, B. Gaudilliere, Y. Yang, K. Shuai, and A. Bonni PIASx Is a MEF2 SUMO E3 Ligase That Promotes Postsynaptic Dendritic Morphogenesis J. Neurosci., September 12, 2007; 27(37): 10037 - 10046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-J. Hamard, M. Boyer-Guittaut, B. Camuzeaux, D. Dujardin, C. Hauss, T. Oelgeschlager, M. Vigneron, C. Kedinger, and B. Chatton Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity Nucleic Acids Res., February 28, 2007; 35(4): 1134 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nowak and M. Hammerschmidt Ubc9 Regulates Mitosis and Cell Survival during Zebrafish Development Mol. Biol. Cell, December 1, 2006; 17(12): 5324 - 5336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tomoiu, A. Gravel, R. M. Tanguay, and L. Flamand Functional Interaction between Human Herpesvirus 6 Immediate-Early 2 Protein and Ubiquitin-Conjugating Enzyme 9 in the Absence of Sumoylation. J. Virol., October 1, 2006; 80(20): 10218 - 10228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gong and E. T. H. Yeh Characterization of a Family of Nucleolar SUMO-specific Proteases with Preference for SUMO-2 or SUMO-3 J. Biol. Chem., June 9, 2006; 281(23): 15869 - 15877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yang, C.-T. Hsu, C.-Y. Ting, L. F. Liu, and J. Hwang Assembly of a Polymeric Chain of SUMO1 on Human Topoisomerase I in Vitro J. Biol. Chem., March 24, 2006; 281(12): 8264 - 8274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sentis, M. Le Romancer, C. Bianchin, M.-C. Rostan, and L. Corbo Sumoylation of the Estrogen Receptor {alpha} Hinge Region Regulates Its Transcriptional Activity Mol. Endocrinol., November 1, 2005; 19(11): 2671 - 2684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Izumiya, T. J. Ellison, E. T. H. Yeh, J. U. Jung, P. A. Luciw, and H.-J. Kung Kaposi's Sarcoma-Associated Herpesvirus K-bZIP Represses Gene Transcription via SUMO Modification J. Virol., August 1, 2005; 79(15): 9912 - 9925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takahashi, S. Hatakeyama, H. Saitoh, and K. I. Nakayama Noncovalent SUMO-1 Binding Activity of Thymine DNA Glycosylase (TDG) Is Required for Its SUMO-1 Modification and Colocalization with the Promyelocytic Leukemia Protein J. Biol. Chem., February 18, 2005; 280(7): 5611 - 5621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cho, B. L. Kagan, J. A. Blackford Jr., D. Szapary, and S. S. Simons Jr. Glucocorticoid Receptor Ligand Binding Domain Is Sufficient for the Modulation of Glucocorticoid Induction Properties by Homologous Receptors, Coactivator Transcription Intermediary Factor 2, and Ubc9 Mol. Endocrinol., February 1, 2005; 19(2): 290 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Yamashita, T. Yamaguchi, M. Shimizu, N. Nakata, F. Hirose, and T. Osumi The transactivating function of peroxisome proliferator-activated receptor {gamma} is negatively regulated by SUMO conjugation in the amino-terminal domain Genes Cells, November 1, 2004; 9(11): 1017 - 1029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Smith, V. Bhaskar, J. Fernandez, and A. J. Courey Drosophila Ulp1, a Nuclear Pore-associated SUMO Protease, Prevents Accumulation of Cytoplasmic SUMO Conjugates J. Biol. Chem., October 15, 2004; 279(42): 43805 - 43814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-K. Chang, Y.-H. Lee, T.-S. Cheng, Y.-R. Hong, P.-J. Lu, J. J. Wang, W.-H. Wang, C.-W. Kuo, S. S.-L. Li, and S.-T. Liu Post-translational Modification of Rta of Epstein-Barr Virus by SUMO-1 J. Biol. Chem., September 10, 2004; 279(37): 38803 - 38812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. O. Vertegaal, S. C. Ogg, E. Jaffray, M. S. Rodriguez, R. T. Hay, J. S. Andersen, M. Mann, and A. I. Lamond A Proteomic Study of SUMO-2 Target Proteins J. Biol. Chem., August 6, 2004; 279(32): 33791 - 33798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yukita, T. Michiue, A. Fukui, K. Sakurai, H. Yamamoto, M. Ihara, A. Kikuchi, and M. Asashima XSENP1, a novel sumo-specific protease in Xenopus, inhibits normal head formation by down-regulation of Wnt/{beta}-catenin signalling Genes Cells, August 1, 2004; 9(8): 723 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheng, D. Wang, Z. Wang, and E. T. H. Yeh SENP1 Enhances Androgen Receptor-Dependent Transcription through Desumoylation of Histone Deacetylase 1 Mol. Cell. Biol., July 1, 2004; 24(13): 6021 - 6028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Castillo, L. J. Kong, L. Hanley-Bowdoin, and E. R. Bejarano Interaction between a Geminivirus Replication Protein and the Plant Sumoylation System J. Virol., March 15, 2004; 78(6): 2758 - 2769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wormald and D. J. Hilton Inhibitors of Cytokine Signal Transduction J. Biol. Chem., January 9, 2004; 279(2): 821 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rosendorff, D. Illanes, G. David, J. Lin, E. Kieff, and E. Johannsen EBNA3C Coactivation with EBNA2 Requires a SUMO Homology Domain J. Virol., January 1, 2004; 78(1): 367 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. H. Mnjoyan, R. Dutta, D. Zhang, B.-B. Teng, and K. Fujise Paradoxical Upregulation of Tumor Suppressor Protein p53 in Serum-Stimulated Vascular Smooth Muscle Cells: A Novel Negative-Feedback Regulatory Mechanism Circulation, July 29, 2003; 108(4): 464 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Lois, C. D. Lima, and N.-H. Chua Small Ubiquitin-Like Modifier Modulates Abscisic Acid Signaling in Arabidopsis PLANT CELL, June 1, 2003; 15(6): 1347 - 1359. [Abstract] [Full Text]
|
|
|
|
|
|
X. Lin, M. Liang, Y.-Y. Liang, F. C. Brunicardi, F. Melchior, and X.-H. Feng Activation of Transforming Growth Factor-{beta} Signaling by SUMO-1 Modification of Tumor Suppressor Smad4/DPC4 J. Biol. Chem., May 23, 2003; 278(21): 18714 - 18719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hirano, S. Murata, K. Tanaka, M. Shimizu, and R. Sato Sterol Regulatory Element-binding Proteins Are Negatively Regulated through SUMO-1 Modification Independent of the Ubiquitin/26 S Proteasome Pathway J. Biol. Chem., May 2, 2003; 278(19): 16809 - 16819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Bencsath, M. S. Podgorski, V. R. Pagala, C. A. Slaughter, and B. A. Schulman Identification of a Multifunctional Binding Site on Ubc9p Required for Smt3p Conjugation J. Biol. Chem., November 27, 2002; 277(49): 47938 - 47945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhang, F. Li, D. Weidner, Z. H. Mnjoyan, and K. Fujise Physical and Functional Interaction between Myeloid Cell Leukemia 1 Protein (MCL1) and Fortilin. THE POTENTIAL ROLE OF MCL1 AS A FORTILIN CHAPERONE J. Biol. Chem., September 27, 2002; 277(40): 37430 - 37438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kotaja, U. Karvonen, O. A. Janne, and J. J. Palvimo PIAS Proteins Modulate Transcription Factors by Functioning as SUMO-1 Ligases Mol. Cell. Biol., July 15, 2002; 22(14): 5222 - 5234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kaul, J. A. Blackford Jr., S. Cho, and S. S. Simons Jr. Ubc9 Is a Novel Modulator of the Induction Properties of Glucocorticoid Receptors J. Biol. Chem., April 5, 2002; 277(15): 12541 - 12549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Li, D. Zhang, and K. Fujise Characterization of Fortilin, a Novel Antiapoptotic Protein J. Biol. Chem., December 7, 2001; 276(50): 47542 - 47549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Kurtzman and N. Schechter Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification PNAS, April 25, 2001; (2001) 101129698. [Abstract] [Full Text]
|
|
|
|
|
|
J.-H. Ahn, Y. Xu, W.-J. Jang, M. J. Matunis, and G. S. Hayward Evaluation of Interactions of Human Cytomegalovirus Immediate-Early IE2 Regulatory Protein with Small Ubiquitin-Like Modifiers and Their Conjugation Enzyme Ubc9 J. Virol., April 15, 2001; 75(8): 3859 - 3872. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. Rochat-Steiner, K. Becker, O. Micheau, P. Schneider, K. Burns, and J. Tschopp FIST/HIPK3: a Fas/FADD-interacting Serine/Threonine Kinase that Induces FADD Phosphorylation and Inhibits Fas-mediated Jun NH2-terminal Kinase Activation J. Exp. Med., October 16, 2000; 192(8): 1165 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wada, E. T. H. Yeh, and T. Kamitani A Dominant-negative UBC12 Mutant Sequesters NEDD8 and Inhibits NEDD8 Conjugation in Vivo J. Biol. Chem., May 26, 2000; 275(22): 17008 - 17015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Li, B. Hesabi, A. Babbo, C. Pacione, J. Liu, D. J. Chen, J. A. Nickoloff, and Z. Shen Regulation of double-strand break-induced mammalian homologous recombination by UBL1, a RAD51-interacting protein Nucleic Acids Res., March 1, 2000; 28(5): 1145 - 1153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gong, S. Millas, G. G. Maul, and E. T. H. Yeh Differential Regulation of Sentrinized Proteins by a Novel Sentrin-specific Protease J. Biol. Chem., February 4, 2000; 275(5): 3355 - 3359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Giorgino, O. de Robertis, L. Laviola, C. Montrone, S. Perrini, K. C. McCowen, and R. J. Smith The sentrin-conjugating enzyme mUbc9 interacts with GLUT4 and GLUT1 glucose transporters and regulates transporter levels in skeletal muscle cells PNAS, February 1, 2000; 97(3): 1125 - 1130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Johnson and G. Blobel Cell Cycle-Regulated Attachment of the Ubiquitin-Related Protein SUMO to the Yeast Septins J. Cell Biol., November 29, 1999; 147(5): 981 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Huggins, M. T. Chin, N. E. S. Sibinga, S.-L. Lee, E. Haber, and M.-E. Lee Characterization of the mUBC9-binding Sites Required for E2A Protein Degradation J. Biol. Chem., October 1, 1999; 274(40): 28690 - 28696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Poukka, P. Aarnisalo, U. Karvonen, J. J. Palvimo, and O. A. Janne Ubc9 Interacts with the Androgen Receptor and Activates Receptor-dependent Transcription J. Biol. Chem., July 2, 1999; 274(27): 19441 - 19446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Johnson, T. E. Spencer, T. R. Hansen, K. J. Austin, R. C. Burghardt, and F. W. Bazer Expression of the Interferon Tau Inducible Ubiquitin Cross-Reactive Protein in the Ovine Uterus Biol Reprod, July 1, 1999; 61(1): 312 - 318. [Abstract] [Full Text]
|
|
|
|
|
|
Q. Liu, C. Jin, X. Liao, Z. Shen, D. J. Chen, and Y. Chen The Binding Interface between an E2 (UBC9) and a Ubiquitin Homologue (UBL1) J. Biol. Chem., June 11, 1999; 274(24): 16979 - 16987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Melnick and J. D. Licht Deconstructing a Disease: RAR{alpha}, Its Fusion Partners, and Their Roles in the Pathogenesis of Acute Promyelocytic Leukemia Blood, May 15, 1999; 93(10): 3167 - 3215. [Full Text] [PDF]
|
|
|
|
|
|
I. Tanida, N. Mizushima, M. Kiyooka, M. Ohsumi, T. Ueno, Y. Ohsumi, and E. Kominami Apg7p/Cvt2p: A Novel Protein-activating Enzyme Essential for Autophagy Mol. Biol. Cell, May 1, 1999; 10(5): 1367 - 1379. [Abstract] [Full Text]
|
|
|
|
|
|
L. Gong and E. T. H. Yeh Identification of the Activating and Conjugating Enzymes of the NEDD8 Conjugation Pathway J. Biol. Chem., April 23, 1999; 274(17): 12036 - 12042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Nawaz, D. M. Lonard, A. P. Dennis, C. L. Smith, and B. W. O'Malley Proteasome-dependent degradation of the human estrogen receptor PNAS, March 2, 1999; 96(5): 1858 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Mizushima, H. Sugita, T. Yoshimori, and Y. Ohsumi A New Protein Conjugation System in Human. THE COUNTERPART OF THE YEAST Apg12p CONJUGATION SYSTEM ESSENTIAL FOR AUTOPHAGY J. Biol. Chem., December 18, 1998; 273(51): 33889 - 33892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kamitani, K. Kito, H. P. Nguyen, H. Wada, T. Fukuda-Kamitani, and E. T. H. Yeh Identification of Three Major Sentrinization Sites in PML J. Biol. Chem., October 9, 1998; 273(41): 26675 - 26682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kamitani, K. Kito, H. P. Nguyen, T. Fukuda-Kamitani, and E. T. H. Yeh Characterization of a Second Member of the Sentrin Family of Ubiquitin-like Proteins J. Biol. Chem., May 1, 1998; 273(18): 11349 - 11353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kamitani, H. P. Nguyen, K. Kito, T. Fukuda-Kamitani, and E. T. H. Yeh Covalent Modification of PML by the Sentrin Family of Ubiquitin-like Proteins J. Biol. Chem., February 6, 1998; 273(6): 3117 - 3120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-i. Kawabe, M. Seki, T. Seki, W.-S. Wang, O. Imamura, Y. Furuichi, H. Saitoh, and T. Enomoto Covalent Modification of the Werner's Syndrome Gene Product with the Ubiquitin-related Protein, SUMO-1 J. Biol. Chem., July 7, 2000; 275(28): 20963 - 20966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mao, S. D. Desai, and L. F. Liu SUMO-1 Conjugation to Human DNA Topoisomerase II Isozymes J. Biol. Chem., August 18, 2000; 275(34): 26066 - 26073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fujise, D. Zhang, J.-l. Liu, and E. T. H. Yeh Regulation of Apoptosis and Cell Cycle Progression by MCL1. DIFFERENTIAL ROLE OF PROLIFERATING CELL NUCLEAR ANTIGEN J. Biol. Chem., December 8, 2000; 275(50): 39458 - 39465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Sampson, M. Wang, and M. J. Matunis The Small Ubiquitin-like Modifier-1 (SUMO-1) Consensus Sequence Mediates Ubc9 Binding and Is Essential for SUMO-1 Modification J. Biol. Chem., June 8, 2001; 276(24): 21664 - 21669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Kurtzman and N. Schechter Ubc9 interacts with a nuclear localization signal and mediates nuclear localization of the paired-like homeobox protein Vsx-1 independent of SUMO-1 modification PNAS, May 8, 2001; 98(10): 5602 - 5607. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
