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Volume 272, Number 45, Issue of November 7, 1997 pp. 28232-28236
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

Interactions among Inactivating and Noninactivating Kv Subunits, and Kv1.2, Produce Potassium Currents with Intermediate Inactivation

(Received for publication, August 11, 1997)

Eric A. Accili , Johann Kiehn , Barbara A. Wible ^** and Arthur M. Brown

From the Rammelkamp Center for Research, MetroHealth Campus, and  Department of Physiology and Biophysics, ^** Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44109-1998

Experiments were carried out to determine whether coinjection of Kv1.2 with inactivating and noninactivating Kv subunits would produce currents with intermediate kinetics and channel complexes containing a mixture of these subunits. Upon coexpression with a saturating amount of Kv1.2 and increasing levels of a noninactivating deletion mutant of Kv1.2, we show that macroscopic Kv1.2 currents have levels of fractional inactivation and inactivation time constants that are intermediate between those obtained with either the inactivating Kv1.2 or the noninactivating Kv1.2 mutant. We also find that coexpression of Kv1.2 with saturating amounts of Kv1.2 and the deletion mutant produces a population of single channels with properties intermediate to either the inactivating or noninactivating parental phenotype. Our data can best be explained by the presence of an intermediate population of heterooligomeric channels consisting of Kv1.2 with different combinations of both types of subunits. Since Kv1.2 subunits coexist in cells with inactivating and noninactivating Kv subunits, our findings suggest that heterooligomeric assembly of these subunits occurs to increase the range of K⁺ current kinetics and expression levels.

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