| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(Received for publication, July 16, 1997, and in revised form, September 9, 1997)
From the The ferrichrome-iron receptor of
Escherichia coli is FhuA, an outer membrane protein that is
dependent upon the energy-coupling protein TonB to enable active
transport of specific hydroxamate siderophores, infection by certain
phages, and cell killing by the protein antibiotics colicin M and
microcin 25. In vivo cross-linking studies were performed
to establish at the biochemical level the interaction between FhuA and
TonB. In an E. coli strain in which both proteins were
expressed from the chromosome, a high molecular mass complex was
detected when the ferrichrome homologue ferricrocin was added
immediately prior to addition of cross-linker. The complex included
both proteins; it was absent from strains of E. coli that
were devoid of either FhuA or TonB, and it was detected with anti-FhuA
and anti-TonB monoclonal antibodies. These results indicate that,
in vivo, the binding of ferricrocin to FhuA enhances
complex formation between the receptor and TonB. An in
vitro system was established with which to examine the FhuA-TonB
interaction. Incubation of TonB with histidine-tagged FhuA followed by
addition of Ni2+-nitrilotriacetate-agarose led to the
specific recovery of both TonB and FhuA. Addition of ferricrocin or
colicin M to FhuA in this system greatly increased the coupling between
FhuA and TonB. Conversely, a monoclonal antibody that binds near the N
terminus of FhuA reduced the retention of TonB by histidine-tagged
FhuA. These studies demonstrate the significance of ligand binding at the external surface of the cell to mediate signal transduction across
the outer membrane.
Volume 272, Number 45,
Issue of November 7, 1997
pp. 28391-28397
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
Cell Envelope Signaling in Escherichia coli
LIGAND BINDING TO THE FERRICHROME-IRON RECEPTOR FhuA PROMOTES
INTERACTION WITH THE ENERGY-TRANSDUCING PROTEIN TonB
,
and
Department of Microbiology and Immunology,
McGill University, Montreal, Quebec H3A 2B4, Canada and the
Department of Microbiology and Department of Biochemistry and
Biophysics, Washington State University,
Pullman, Washington 99164-4233
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kim, G. E. Fanucci, and D. S. Cafiso Substrate-dependent transmembrane signaling in TonB-dependent transporters is not conserved PNAS, July 17, 2007; 104(29): 11975 - 11980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Adams, G. Zeder-Lutz, I. Schalk, F. Pattus, and H. Celia Interaction of TonB with the Outer Membrane Receptor FpvA of Pseudomonas aeruginosa. J. Bacteriol., August 1, 2006; 188(16): 5752 - 5761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kodding, F. Killig, P. Polzer, S. P. Howard, K. Diederichs, and W. Welte Crystal Structure of a 92-Residue C-terminal Fragment of TonB from Escherichia coli Reveals Significant Conformational Changes Compared to Structures of Smaller TonB Fragments J. Biol. Chem., January 28, 2005; 280(4): 3022 - 3028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Khursigara, G. De Crescenzo, P. D. Pawelek, and J. W. Coulton Enhanced Binding of TonB to a Ligand-loaded Outer Membrane Receptor: ROLE OF THE OLIGOMERIC STATE OF TonB IN FORMATION OF A FUNCTIONAL FhuA{middle dot}TonB COMPLEX J. Biol. Chem., February 27, 2004; 279(9): 7405 - 7412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sauter, S. P. Howard, and V. Braun In Vivo Evidence for TonB Dimerization J. Bacteriol., October 1, 2003; 185(19): 5747 - 5754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cadieux, P. G. Phan, D. S. Cafiso, and R. J. Kadner Differential substrate-induced signaling through the TonB-dependent transporter BtuB PNAS, September 16, 2003; 100(19): 10688 - 10693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Endriss, M. Braun, H. Killmann, and V. Braun Mutant Analysis of the Escherichia coli FhuA Protein Reveals Sites of FhuA Activity J. Bacteriol., August 15, 2003; 185(16): 4683 - 4692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ogierman and V. Braun Interactions between the Outer Membrane Ferric Citrate Transporter FecA and TonB: Studies of the FecA TonB Box J. Bacteriol., March 15, 2003; 185(6): 1870 - 1885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Mey and S. M. Payne Analysis of Residues Determining Specificity of Vibrio cholerae TonB1 for Its Receptors J. Bacteriol., February 15, 2003; 185(4): 1195 - 1207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Killmann, C. Herrmann, A. Torun, G. Jung, and V. Braun TonB of Escherichia coli activates FhuA through interaction with the {beta}-barrel Microbiology, November 1, 2002; 148(11): 3497 - 3509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. G. Mikael, P. D. Pawelek, J. Labrie, M. Sirois, J. W. Coulton, and M. Jacques Molecular cloning and characterization of the ferric hydroxamate uptake (fhu) operon in Actinobacillus pleuropneumoniae Microbiology, September 1, 2002; 148(9): 2869 - 2882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhao and K. Poole Mutational Analysis of the TonB1 Energy Coupler of Pseudomonas aeruginosa J. Bacteriol., March 15, 2002; 184(6): 1503 - 1513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. I. Higgs, T. E. Letain, K. K. Merriam, N. S. Burke, H. Park, C. Kang, and K. Postle TonB Interacts with Nonreceptor Proteins in the Outer Membrane of Escherichia coli J. Bacteriol., March 15, 2002; 184(6): 1640 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Ferguson, R. Chakraborty, B. S. Smith, L. Esser, D. van der Helm, and J. Deisenhofer Structural Basis of Gating by the Outer Membrane Transporter FecA Science, March 1, 2002; 295(5560): 1715 - 1719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Howard, C. Herrmann, C. W. Stratilo, and V. Braun In Vivo Synthesis of the Periplasmic Domain of TonB Inhibits Transport through the FecA and FhuA Iron Siderophore Transporters of Escherichia coli J. Bacteriol., October 15, 2001; 183(20): 5885 - 5895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Killmann, M. Braun, C. Herrmann, and V. Braun FhuA Barrel-Cork Hybrids Are Active Transporters and Receptors J. Bacteriol., June 1, 2001; 183(11): 3476 - 3487. [Abstract] [Full Text]
|
|
|
|
|
|
G. S. Moeck and L. Letellier Characterization of In Vitro Interactions between a Truncated TonB Protein from Escherichia coli and the Outer Membrane Receptors FhuA and FepA J. Bacteriol., May 1, 2001; 183(9): 2755 - 2764. [Abstract] [Full Text]
|
|
|
|
|
|
X.-Z. Li and K. Poole Mutational Analysis of the OprM Outer Membrane Component of the MexA-MexB-OprM Multidrug Efflux System of Pseudomonas aeruginosa J. Bacteriol., January 1, 2001; 183(1): 12 - 27. [Abstract] [Full Text]
|
|
|
|
|
|
N. Cadieux, C. Bradbeer, and R. J. Kadner Sequence Changes in the Ton Box Region of BtuB Affect Its Transport Activities and Interaction with TonB Protein J. Bacteriol., November 1, 2000; 182(21): 5954 - 5961. [Abstract] [Full Text]
|
|
|
|
|
|
C. S. Bracken, M. T. Baer, A. Abdur-Rashid, W. Helms, and I. Stojiljkovic Use of Heme-Protein Complexes by the Yersinia enterocolitica HemR Receptor: Histidine Residues Are Essential for Receptor Function J. Bacteriol., October 1, 1999; 181(19): 6063 - 6072. [Abstract] [Full Text]
|
|
|
|
|
|
N. Cadieux and R. J. Kadner Site-directed disulfide bonding reveals an interaction site between energy-coupling protein TonB and BtuB, the outer membrane cobalamin transporter PNAS, September 14, 1999; 96(19): 10673 - 10678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Possot, M. Gérard-Vincent, and A. P. Pugsley Membrane Association and Multimerization of Secreton Component PulC J. Bacteriol., July 1, 1999; 181(13): 4004 - 4011. [Abstract] [Full Text]
|
|
|
|
|
|
S. Bleves, M. Gérard-Vincent, A. Lazdunski, and A. Filloux Structure-Function Analysis of XcpP, a Component Involved in General Secretory Pathway-Dependent Protein Secretion in Pseudomonas aeruginosa J. Bacteriol., July 1, 1999; 181(13): 4012 - 4019. [Abstract] [Full Text]
|
|
|
|
|
|
A. D. Ferguson, E. Hofmann, J. W. Coulton, K. Diederichs, and W. Welte Siderophore-Mediated Iron Transport: Crystal Structure of FhuA with Bound Lipopolysaccharide Science, December 18, 1998; 282(5397): 2215 - 2220. [Abstract] [Full Text]
|
|
|
|
|
|
P. I. Higgs, P. S. Myers, and K. Postle Interactions in the TonB-Dependent Energy Transduction Complex: ExbB and ExbD Form Homomultimers J. Bacteriol., November 15, 1998; 180(22): 6031 - 6038. [Abstract] [Full Text]
|
|
|
|
|
|
H. Killmann, C. Herrmann, H. Wolff, and V. Braun Identification of a New Site for Ferrichrome Transport by Comparison of the FhuA Proteins of Escherichia coli, Salmonella paratyphi B, Salmonella typhimurium, and Pantoea agglomerans J. Bacteriol., August 1, 1998; 180(15): 3845 - 3852. [Abstract] [Full Text]
|
|
|
|
|
|
R. A. Larsen and K. Postle Conserved Residues Ser16 and His20 and Their Relative Positioning Are Essential for TonB Activity, Cross-linking of TonB with ExbB, and the Ability of TonB to Respond to Proton Motive Force J. Biol. Chem., March 9, 2001; 276(11): 8111 - 8117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Scott, Z. Cao, Z. Qi, M. Bauler, J. D. Igo, S. M. C. Newton, and P. E. Klebba Exchangeability of N Termini in the Ligand-gated Porins of Escherichia coli J. Biol. Chem., April 13, 2001; 276(16): 13025 - 13033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chang, A. Mooser, A. Pluckthun, and A. Wlodawer Crystal Structure of the Dimeric C-terminal Domain of TonB Reveals a Novel Fold J. Biol. Chem., July 13, 2001; 276(29): 27535 - 27540. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
