HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by He, C.-Z. Articles by Burch, J. B. E. Search for Related Content PubMed PubMed Citation Articles by He, C.-Z. Articles by Burch, J. B. E. Social Bookmarking                      What's this?

Volume 272, Number 45, Issue of November 7, 1997 pp. 28550-28556
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

The Chicken GATA-6 Locus Contains Multiple Control Regions That Confer Distinct Patterns of Heart Region-specific Expression in Transgenic Mouse Embryos

(Received for publication, June 17, 1997, and in revised form, July 30, 1997)

From the Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111

The GATA-6 transcription factor is expressed in cardiogenic cells and during subsequent stages of heart development in diverse vertebrate species. To gain insights into the molecular events that govern this heart-restricted expression, we isolated the chicken GATA-6 gene and used several approaches to screen for associated control regions. Our analysis of two chicken GATA-6/lacZ constructs in transgenic mouse embryos was particularly revealing. One GATA-6/lacZ construct, which has 1.5 kilobase pairs of upstream sequences along with the promoter and first intron, was expressed exclusively in the atrioventricular canal region of the heart. This expression pattern is novel and appears to mark specialized myocardial cells that induce underlying endocardial cells to initiate valve formation. The other GATA-6/lacZ construct, which has an additional 7.7 kilobase pairs of upstream sequences, was expressed in the ventricle and outflow tract in addition to the atrioventricular canal. The failure of these GATA-6 control regions to function as enhancers in transfected cardiac myocyte cultures underscores the importance of using transgenic approaches to elucidate transcriptional controls that function in the developing heart. Although the endogenous GATA-6 gene is expressed throughout the heart, our results indicate that this is effected in a heart region-specific manner.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. A. Eisenberg, J. B.E. Burch, and L. M. Eisenberg Bone Marrow Cells Transdifferentiate to Cardiomyocytes When Introduced into the Embryonic Heart Stem Cells, May 1, 2006; 24(5): 1236 - 1245. [Abstract] [Full Text] [PDF]

				V. Gaussin, G. E. Morley, L. Cox, A. Zwijsen, K. M. Vance, L. Emile, Y. Tian, J. Liu, C. Hong, D. Myers, et al. Alk3/Bmpr1a Receptor Is Required for Development of the Atrioventricular Canal Into Valves and Annulus Fibrosus Circ. Res., August 5, 2005; 97(3): 219 - 226. [Abstract] [Full Text] [PDF]

				C. D. Capo-chichi, I. H. Roland, L. Vanderveer, R. Bao, T. Yamagata, H. Hirai, C. Cohen, T. C. Hamilton, A. K. Godwin, and X.-X. Xu Anomalous Expression of Epithelial Differentiation-determining GATA Factors in Ovarian Tumorigenesis Cancer Res., August 15, 2003; 63(16): 4967 - 4977. [Abstract] [Full Text] [PDF]

				P. E.M.H. Habets, A. F.M. Moorman, and V. M. Christoffels Regulatory modules in the developing heart Cardiovasc Res, May 1, 2003; 58(2): 246 - 263. [Abstract] [Full Text] [PDF]

				E.M. SMALL and P.A. KRIEG Molecular Mechanisms of Chamber-specific Myocardial Gene Expression: Transgenic Analysis of the ANF Promoter Cold Spring Harb Symp Quant Biol, January 1, 2002; 67(0): 71 - 80. [Abstract] [PDF]

				J. D. Croissant, S. Carpenter, and D. Bader Identification and Genomic Cloning of CMHC1. A UNIQUE MYOSIN HEAVY CHAIN EXPRESSED EXCLUSIVELY IN THE DEVELOPING CHICKEN HEART J. Biol. Chem., January 21, 2000; 275(3): 1944 - 1951. [Abstract] [Full Text] [PDF]

				A. Brewer, C. Gove, A. Davies, C. McNulty, D. Barrow, M. Koutsourakis, F. Farzaneh, J. Pizzey, A. Bomford, and R. Patient The Human and Mouse GATA-6 Genes Utilize Two Promoters and Two Initiation Codons J. Biol. Chem., December 31, 1999; 274(53): 38004 - 38016. [Abstract] [Full Text] [PDF]

				H Kuo, J Chen, P Ruiz-Lozano, Y Zou, M Nemer, and K. Chien Control of segmental expression of the cardiac-restricted ankyrin repeat protein gene by distinct regulatory pathways in murine cardiogenesis Development, January 10, 1999; 126(19): 4223 - 4234. [Abstract] [PDF]

				J. Reecy, X Li, M Yamada, F. DeMayo, C. Newman, R. Harvey, and R. Schwartz Identification of upstream regulatory regions in the heart-expressed homeobox gene Nkx2-5 Development, January 2, 1999; 126(4): 839 - 849. [Abstract] [PDF]

				R. Searcy, E. Vincent, C. Liberatore, and K. Yutzey A GATA-dependent nkx-2.5 regulatory element activates early cardiac gene expression in transgenic mice Development, January 11, 1998; 125(22): 4461 - 4470. [Abstract] [PDF]