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(Received for publication, March 3, 1997, and in revised form, August 4, 1997)
From the Department of Pathology and Laboratory Medicine,
University of Cincinnati College of Medicine,
Cincinnati, Ohio 45267-0529
Reverse transcriptase-polymerase chain reaction
was used to study the biosynthesis of two different cholesteryl ester
hydrolases by human and mouse macrophages. Oligonucleotide primers for
bile salt-stimulated cholesterol esterase yielded positive reactions with RNA isolated from human peripheral blood monocytes,
monocyte-derived macrophages, the human monocytic THP-1 cells, and
phorbol ester-induced THP-1 macrophages. In contrast, oligonucleotide
primers for hormone-sensitive lipase yielded positive reactions only
with RNA isolated from non-differentiated human THP-1 monocytic cells
and peripheral blood monocytes, but not those obtained from
differentiated THP-1 macrophages or monocyte-derived macrophages. Thus,
while human monocytes were capable of synthesizing both enzymes, human
macrophages synthesized only bile salt-stimulated cholesterol esterase
and not the hormone-sensitive lipase. The synthesis of bile
salt-stimulated cholesterol esterase by human macrophages was confirmed
by detection of bile salt-stimulated cholesteryl ester hydrolytic
activity in conditioned media of differentiated THP-1 cells and human
peripheral blood monocyte-derived macrophages. Moreover, incubating
human macrophages with oxidized low density lipoprotein (LDL) or
acetylated LDL increased bile salt-stimulated cholesterol esterase
activity in the conditioned media of these cells. These results with
human macrophages were contrasted with results of studies with mouse macrophages, which showed the presence of hormone-sensitive lipase mRNA but not the bile salt-stimulated cholesterol esterase
mRNA. Taken together, these results demonstrated species-specific
differences in expression of cholesteryl ester hydrolytic enzymes in
macrophages. The expression of bile salt-stimulated cholesterol
esterase by human macrophages, in a process inducible by modified LDL,
suggests a role of this protein in atherogenesis.
Volume 272, Number 45,
Issue of November 7, 1997
pp. 28666-28671
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
Modified Low Density Lipoprotein Enhances the Secretion of
Bile Salt-stimulated Cholesterol Esterase by Human
Monocyte-Macrophages
SPECIES-SPECIFIC DIFFERENCE IN MACROPHAGE CHOLESTERYL ESTER
HYDROLASE
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