Volume 272, Number 47, Issue of November 21, 1997 pp. 29403-29406
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.

COMMUNICATION:
A Family of Putative Tumor Suppressors Is Structurally and Functionally Conserved in Humans and Yeast

(Received for publication, August 15, 1997, and in revised form, September 15, 1997)

Liwu Li , Brian R. Ernsting , Matthew J. Wishart ^¶ , Daniel L. Lohse and Jack E. Dixon

From the Departments of  Biological Chemistry and ^¶ Physiology, University of Michigan, Ann Arbor, Michigan 48109-0606

In Saccharomyces cerevisiae the CDC14 gene is essential for cell cycle progression. Strains carrying the cdc14-1^ts allele enter the cell cycle and arrest at restrictive temperatures. We have identified two human cDNAs encoding proteins which share sequence identity to the yeast CDC14p. The cell cycle arrest in cdc14-1^ts can be specifically complemented by the human cDNAs suggesting that they are functionally equivalent to the yeast CDC14 gene. Another clone identified in the search for human CDC14-like proteins corresponded to the putative tumor suppressor gene PTEN/MMAC1 (phosphatase and tensin homologue deleted on chromosome 10 or mutated in multiple advanced cancers 1). Analysis of the PTEN/MMAC1 showed that it did not complement the cdc14-1^ts allele and that it is more closely related to the yeast open reading frame YNL128W. Human CDC14p and PTEN/MMAC1 were expressed as recombinant proteins, and both were shown to have kinetic properties characteristic of dual specific phosphatases. The human CDC14p was localized in the nucleus while PTEN/MMAC1 has been reported to be localized in the cytoplasm. Our results suggest that CDC14 and YNL128W/PTEN/MMAC1 represent two related, but distinct, families of human and yeast phosphatases.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C.-J. Chang, D. J. Mulholland, B. Valamehr, S. Mosessian, W. R. Sellers, and H. Wu PTEN Nuclear Localization Is Regulated by Oxidative Stress and Mediates p53-Dependent Tumor Suppression Mol. Cell. Biol., May 15, 2008; 28(10): 3281 - 3289. [Abstract] [Full Text] [PDF]

				J. Wu, H. P. Cho, D. B. Rhee, D. K. Johnson, J. Dunlap, Y. Liu, and Y. Wang Cdc14B depletion leads to centriole amplification, and its overexpression prevents unscheduled centriole duplication J. Cell Biol., May 1, 2008; 181(3): 475 - 483. [Abstract] [Full Text] [PDF]

				L. Lanzetti, V. Margaria, F. Melander, L. Virgili, M.-H. Lee, J. Bartek, and S. Jensen Regulation of the Rab5 GTPase-activating Protein RN-tre by the Dual Specificity Phosphatase Cdc14A in Human Cells J. Biol. Chem., May 18, 2007; 282(20): 15258 - 15270. [Abstract] [Full Text] [PDF]

				M. D. Vazquez-Novelle, V. Esteban, A. Bueno, and M. P. Sacristan Functional Homology among Human and Fission Yeast Cdc14 Phosphatases J. Biol. Chem., August 12, 2005; 280(32): 29144 - 29150. [Abstract] [Full Text] [PDF]

				H. P. Cho, Y. Liu, M. Gomez, J. Dunlap, M. Tyers, and Y. Wang The Dual-Specificity Phosphatase CDC14B Bundles and Stabilizes Microtubules Mol. Cell. Biol., June 1, 2005; 25(11): 4541 - 4551. [Abstract] [Full Text] [PDF]

				R. Jessberger How To Divorce Engaged Chromosomes? Mol. Cell. Biol., January 1, 2005; 25(1): 18 - 22. [Full Text] [PDF]

				D. D'Amours and A. Amon At the interface between signaling and executing anaphase--Cdc14 and the FEAR network Genes & Dev., November 1, 2004; 18(21): 2581 - 2595. [Abstract] [Full Text] [PDF]

				W.-Q. Wang, J. Bembenek, K. R. Gee, H. Yu, H. Charbonneau, and Z.-Y. Zhang Kinetic and Mechanistic Studies of a Cell Cycle Protein Phosphatase Cdc14 J. Biol. Chem., July 16, 2004; 279(29): 30459 - 30468. [Abstract] [Full Text] [PDF]

				V. Esteban, M. Blanco, N. Cueille, V. Simanis, S. Moreno, and A. Bueno A role for the Cdc14-family phosphatase Flp1p at the end of the cell cycle in controlling the rapid degradation of the mitotic inducer Cdc25p in fission yeast J. Cell Sci., May 15, 2004; 117(12): 2461 - 2468. [Abstract] [Full Text] [PDF]

				L. Mahimainathan and G. G. Choudhury Inactivation of Platelet-derived Growth Factor Receptor by the Tumor Suppressor PTEN Provides a Novel Mechanism of Action of the Phosphatase J. Biol. Chem., April 9, 2004; 279(15): 15258 - 15268. [Abstract] [Full Text] [PDF]

				S. C. Dryden, F. A. Nahhas, J. E. Nowak, A.-S. Goustin, and M. A. Tainsky Role for Human SIRT2 NAD-Dependent Deacetylase Activity in Control of Mitotic Exit in the Cell Cycle Mol. Cell. Biol., May 1, 2003; 23(9): 3173 - 3185. [Abstract] [Full Text] [PDF]

				M. E. Ginn-Pease and C. Eng Increased Nuclear Phosphatase and Tensin Homologue Deleted on Chromosome 10 Is Associated with G0-G1 in MCF-7 Cells Cancer Res., January 15, 2003; 63(2): 282 - 286. [Abstract] [Full Text] [PDF]

				U. Gruneberg, M. Glotzer, A. Gartner, and E. A. Nigg The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo J. Cell Biol., September 3, 2002; 158(5): 901 - 914. [Abstract] [Full Text] [PDF]

				B. K. Kaiser, Z. A. Zimmerman, H. Charbonneau, and P. K. Jackson Disruption of Centrosome Structure, Chromosome Segregation, and Cytokinesis by Misexpression of Human Cdc14A Phosphatase Mol. Biol. Cell, July 1, 2002; 13(7): 2289 - 2300. [Abstract] [Full Text] [PDF]

				D. A. Guertin, S. Trautmann, and D. McCollum Cytokinesis in Eukaryotes Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 155 - 178. [Abstract] [Full Text] [PDF]

				N. Cueille, E. Salimova, V. Esteban, M. Blanco, S. Moreno, A. Bueno, and V. Simanis Flp1, a fission yeast orthologue of the S. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis J. Cell Sci., March 9, 2002; 114(14): 2649 - 2664. [Abstract] [Full Text] [PDF]

				J. Shou, R. Soriano, S. W. Hayward, G. R. Cunha, P. M. Williams, and W.-Q. Gao Expression profiling of a human cell line model of prostatic cancer reveals a direct involvement of interferon signaling in prostate tumor progression PNAS, March 5, 2002; 99(5): 2830 - 2835. [Abstract] [Full Text] [PDF]

				J. Bembenek and H. Yu Regulation of the Anaphase-promoting Complex by the Dual Specificity Phosphatase Human Cdc14a J. Biol. Chem., December 14, 2001; 276(51): 48237 - 48242. [Abstract] [Full Text] [PDF]

				A. C. Carrano and M. Pagano Role of the F-Box Protein Skp2 in Adhesion-dependent Cell Cycle Progression J. Cell Biol., June 18, 2001; 153(7): 1381 - 1390. [Abstract] [Full Text] [PDF]

				J. Heymont, L. Berenfeld, J. Collins, A. Kaganovich, B. Maynes, A. Moulin, I. Ratskovskaya, P. P. Poon, G. C. Johnston, M. Kamenetsky, et al. TEP1, the yeast homolog of the human tumor suppressor gene PTEN/MMAC1/TEP1, is linked to the phosphatidylinositol pathway and plays a role in the developmental process of sporulation PNAS, November 7, 2000; 97(23): 12672 - 12677. [Abstract] [Full Text] [PDF]

				A. I. Flores, B. S. Mallon, T. Matsui, W. Ogawa, A. Rosenzweig, T. Okamoto, and W. B. Macklin Akt-Mediated Survival of Oligodendrocytes Induced by Neuregulins J. Neurosci., October 15, 2000; 20(20): 7622 - 7630. [Abstract] [Full Text] [PDF]

				L. Li, M. Ljungman, and J. E. Dixon The Human Cdc14 Phosphatases Interact with and Dephosphorylate the Tumor Suppressor Protein p53 J. Biol. Chem., January 28, 2000; 275(4): 2410 - 2414. [Abstract] [Full Text] [PDF]

				W. E. Hughes, R. Woscholski, F. T. Cooke, R. S. Patrick, S. K. Dove, N. Q. McDonald, and P. J. Parker SAC1 Encodes a Regulated Lipid Phosphoinositide Phosphatase, Defects in Which Can Be Suppressed by the Homologous Inp52p and Inp53p Phosphatases J. Biol. Chem., January 14, 2000; 275(2): 801 - 808. [Abstract] [Full Text] [PDF]

				D. C.I. Goberdhan, N. Paricio, E. C. Goodman, M. Mlodzik, and C. Wilson Drosophila tumor suppressor PTEN controls cell size and number by antagonizing the Chico/PI3-kinase signaling pathway Genes & Dev., December 15, 1999; 13(24): 3244 - 3258. [Abstract] [Full Text]

				M. Tamura, J. Gu, H. Tran, and K. M. Yamada PTEN Gene and Integrin Signaling in Cancer J Natl Cancer Inst, November 3, 1999; 91(21): 1820 - 1828. [Abstract] [Full Text] [PDF]

				W. Zachariae and K. Nasmyth Whose end is destruction: cell division and the anaphase-promoting complex Genes & Dev., August 15, 1999; 13(16): 2039 - 2058. [Full Text]

				A. E. Rudolph, J. A. Stuckey, Y. Zhao, H. R. Matthews, W. A. Patton, J. Moss, and J. E. Dixon Expression, Characterization, and Mutagenesis of the Yersinia pestis Murine Toxin, a Phospholipase D Superfamily Member J. Biol. Chem., April 23, 1999; 274(17): 11824 - 11831. [Abstract] [Full Text] [PDF]

				L. C. Cantley and B. G. Neel New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway PNAS, April 13, 1999; 96(8): 4240 - 4245. [Abstract] [Full Text] [PDF]

				H Huang, C. Potter, W Tao, D. Li, W Brogiolo, E Hafen, H Sun, and T Xu PTEN affects cell size, cell proliferation and apoptosis during Drosophila eye development Development, January 12, 1999; 126(23): 5365 - 5372. [Abstract] [PDF]

				M. Tamura, J. Gu, T. Takino, and K. M. Yamada Tumor Suppressor PTEN Inhibition of Cell Invasion, Migration, and Growth: Differential Involvement of Focal Adhesion Kinase and p130Cas Cancer Res., January 1, 1999; 59(2): 442 - 449. [Abstract] [Full Text] [PDF]

				M. Tamura, J. Gu, K. Matsumoto, S. Aota, R. Parsons, and K. M. Yamada Inhibition of Cell Migration, Spreading, and Focal Adhesions by Tumor Suppressor PTEN Science, June 5, 1998; 280(5369): 1614 - 1617. [Abstract] [Full Text]

				T. Maehama and J. E. Dixon The Tumor Suppressor, PTEN/MMAC1, Dephosphorylates the Lipid Second Messenger, Phosphatidylinositol 3,4,5-Trisphosphate J. Biol. Chem., May 29, 1998; 273(22): 13375 - 13378. [Abstract] [Full Text] [PDF]

				Q. Zeng, X. Si, H. Horstmann, Y. Xu, W. Hong, and C. J. Pallen Prenylation-dependent Association of Protein-tyrosine Phosphatases PRL-1, -2, and -3 with the Plasma Membrane and the Early Endosome J. Biol. Chem., July 7, 2000; 275(28): 21444 - 21452. [Abstract] [Full Text] [PDF]

				E. E. Traverso, C. Baskerville, Y. Liu, W. Shou, P. James, R. J. Deshaies, and H. Charbonneau Characterization of the Net1 Cell Cycle-dependent Regulator of the Cdc14 Phosphatase from Budding Yeast J. Biol. Chem., June 8, 2001; 276(24): 21924 - 21931. [Abstract] [Full Text] [PDF]