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(Received for publication, August 14, 1997, and in revised form, September 10, 1997)
From the Department of Biochemistry, Uniformed Services University
of the Health Sciences, Bethesda, Maryland 20814
The Saccharomyces cerevisiae SCS7 and
SUR2 genes are members of a gene family that encodes
enzymes that desaturate or hydroxylate lipids. Sur2p is required for
the hydroxylation of C-4 of the sphingoid moiety of ceramide, and Scs7p
is required for the hydroxylation of the very long chain fatty acid.
Neither SCS7 nor SUR2 are essential for growth,
and lack of the Scs7p- or Sur2p-dependent hydroxylation does not prevent the synthesis of mannosyldiinositolphosphorylceramide, the mature sphingolipid found in yeast. Deletion of either gene suppresses the Ca2+-sensitive phenotype of
csg2
Volume 272, Number 47,
Issue of November 21, 1997
pp. 29704-29710
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
Hydroxylation of Saccharomyces cerevisiae Ceramides
Requires Sur2p and Scs7p
mutants, which arises from overaccumulation of
inositolphosphorylceramide due to a defect in sphingolipid mannosylation. Characterization of scs7 and
sur2 mutants is expected to provide insight into the
function of ceramide hydroxylation.
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