|
|
|
|||||||
|
|||||||||
(Received for publication, July 2, 1997, and in revised form, September 23, 1997)
From the Department of Zoology, Auburn University,
Auburn, Alabama 36849-5414
We have previously shown that protein kinase C
(PKC)-
Volume 272, Number 49,
Issue of December 5, 1997
pp. 31130-31137
©1997 by The American Society for Biochemistry and Molecular Biology, Inc.
Nucleolin Is a Protein Kinase C-
Substrate
CONNECTION BETWEEN CELL SURFACE SIGNALING AND NUCLEUS IN PC12
CELLS
is activated and required for nerve growth factor
(NGF)-induced differentiation of rat pheochromocytoma PC12 cells
(Wooten, M. W., Zhou, G., Seibenhener, M. L., and Coleman,
E. S. (1994) Cell Growth & Diff. 5, 395-403; Coleman,
E. S., and Wooten, M. W. (1994) J. Mol. Neurosci.
5, 39-57). Here we report the characterization and identification of a
106-kDa nuclear protein as a specific substrate of PKC-. NGF
treatment of PC12 cells resulted in translocation of PKC- and
coincident phosphorylation of a protein that was localized within the
nucleoplasm of nuclei isolated from PC12 cells. Addition of PKC-
pseudosubstrate peptide in vitro or myristoylated peptide in vivo diminished phosphorylation of pp106 in a
dose-dependent fashion. Likewise, addition of purified
PKC-, but neither PKC-
nor 
, to nuclear extracts resulted in
an incremental increase in the phosphorylation of pp106. Expression of
dominant-negative PKC- inhibited NGF-induced phosphorylation of
pp106, by comparison overexpression of PKC- enhanced basal
phosphorylation without a noticeable effect upon NGF-induced effects.
Amino acid sequence analysis of four peptides derived from purified
pp106 revealed that this protein was homologous to nucleolin. Using an
in vitro reconstitution system, purified nucleolin was
likewise shown to be phosphorylated by purified PKC-. The staining
intensity of both enzyme and substrate in the nucleus increased upon
treatment with NGF. In vivo labeling with
32Pi and stimulation of PC12 cells with NGF
followed by immunoprecipitation with anti-nucleolin antibody
corroborated the in vitro approach documenting enhanced
phosphorylation of nucleolin by NGF treatment. Taken together, the
findings presented herein document that nucleolin is a target of
PKC- that serves to relay NGF signals from cell surface to nucleus
in PC12 cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Guo, S. Wu, L. Wang, R.-y. Wang, X. Wei, J. Liu, and B. Fang Interruption of RNA processing machinery by a small compound, 1-[(4-chlorophenyl)methyl]-1H-indole-3-carboxaldehyde (oncrasin-1) Mol. Cancer Ther., February 1, 2009; 8(2): 441 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Wolf, A. I. Lyuksyutova, A. G. Fenstermaker, B. Shafer, C. G. Lo, and Y. Zou Phosphatidylinositol-3-Kinase-Atypical Protein Kinase C Signaling Is Required for Wnt Attraction and Anterior-Posterior Axon Guidance J. Neurosci., March 26, 2008; 28(13): 3456 - 3467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Iakoubov, A. Izzo, A. Yeung, C. I. Whiteside, and P. L. Brubaker Protein Kinase C{zeta} Is Required for Oleic Acid-Induced Secretion of Glucagon-Like Peptide-1 by Intestinal Endocrine L Cells Endocrinology, March 1, 2007; 148(3): 1089 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Krejci, B. Masri, L. Salazar, C. Farrington-Rock, H. Prats, L. M. Thompson, and W. R. Wilcox Bisindolylmaleimide I Suppresses Fibroblast Growth Factor-mediated Activation of Erk MAP Kinase in Chondrocytes by Preventing Shp2 Association with the Frs2 and Gab1 Adaptor Proteins J. Biol. Chem., February 2, 2007; 282(5): 2929 - 2936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Godeny and P. P. Sayeski ANG II-induced cell proliferation is dually mediated by c-Src/Yes/Fyn-regulated ERK1/2 activation in the cytoplasm and PKC{zeta}-controlled ERK1/2 activity within the nucleus Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1297 - C1307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Girvan, Y. Teng, L. K. Casson, S. D. Thomas, S. Juliger, M. W. Ball, J. B. Klein, W. M. Pierce Jr., S. S. Barve, and P. J. Bates AGRO100 inhibits activation of nuclear factor-{kappa}B (NF-{kappa}B) by forming a complex with NF-{kappa}B essential modulator (NEMO) and nucleolin. Mol. Cancer Ther., July 1, 2006; 5(7): 1790 - 1799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. B. Hickey, K. England, and T. G. Cotter Bcr-Abl regulates osteopontin transcription via Ras, PI-3K, aPKC, Raf-1, and MEK J. Leukoc. Biol., July 1, 2005; 78(1): 289 - 300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, D. E. Handy, and J. Loscalzo Adenosine-Dependent Induction of Glutathione Peroxidase 1 in Human Primary Endothelial Cells and Protection Against Oxidative Stress Circ. Res., April 29, 2005; 96(8): 831 - 837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hardy, G. G. St-Onge, E. Joly, Y. Langelier, and M. Prentki Oleate Promotes the Proliferation of Breast Cancer Cells via the G Protein-coupled Receptor GPR40 J. Biol. Chem., April 8, 2005; 280(14): 13285 - 13291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Sinclair and A. D. O'Brien Intimin Types {alpha}, {beta}, and {gamma} Bind to Nucleolin with Equivalent Affinity but Lower Avidity than to the Translocated Intimin Receptor J. Biol. Chem., August 6, 2004; 279(32): 33751 - 33758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. Sengupta, S. Bandyopadhyay, D. J. Fernandes, and E. K. Spicer Identification of Nucleolin as an AU-rich Element Binding Protein Involved in bcl-2 mRNA Stabilization J. Biol. Chem., March 19, 2004; 279(12): 10855 - 10863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xie, M. Singh, D. A. Siwik, W. L. Joyner, and K. Singh Osteopontin Inhibits Interleukin-1{beta}-stimulated Increases in Matrix Metalloproteinase Activity in Adult Rat Cardiac Fibroblasts: ROLE OF PROTEIN KINASE C-{zeta} J. Biol. Chem., December 5, 2003; 278(49): 48546 - 48552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Suzuki, K. Akimoto, and S. Ohno Protein Kinase C {lambda}/{iota} (PKC{lambda}/{iota}): A PKC Isotype Essential for the Development of Multicellular Organisms J. Biochem., January 1, 2003; 133(1): 9 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. O. Esteve, E. Chicoine, O. Robledo, F. Aoudjit, A. Descoteaux, E. F. Potworowski, and Y. St-Pierre Protein Kinase C-zeta Regulates Transcription of the Matrix Metalloproteinase-9 Gene Induced by IL-1 and TNF-alpha in Glioma Cells via NF-kappa B J. Biol. Chem., September 13, 2002; 277(38): 35150 - 35155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Morimoto, H. Okamura, and T. Haneji Interaction of Protein Phosphatase 1 Delta with Nucleolin in Human Osteoblastic Cells J. Histochem. Cytochem., September 1, 2002; 50(9): 1187 - 1193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zhou, K. A. Mihindukulasuriya, R. A. MacCorkle-Chosnek, A. Van Hooser, M. C.-T. Hu, B. R. Brinkley, and T.-H. Tan Protein Phosphatase 4 Is Involved in Tumor Necrosis Factor-alpha -induced Activation of c-Jun N-terminal Kinase J. Biol. Chem., February 15, 2002; 277(8): 6391 - 6398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Wooten, M. L. Vandenplas, M. L. Seibenhener, T. Geetha, and M. T. Diaz-Meco Nerve Growth Factor Stimulates Multisite Tyrosine Phosphorylation and Activation of the Atypical Protein Kinase C's via a src Kinase Pathway Mol. Cell. Biol., December 15, 2001; 21(24): 8414 - 8427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Buteau, S. Foisy, C. J. Rhodes, L. Carpenter, T. J. Biden, and M. Prentki Protein Kinase C{zeta} Activation Mediates Glucagon-Like Peptide-1-Induced Pancreatic {beta}-Cell Proliferation Diabetes, October 1, 2001; 50(10): 2237 - 2243. [Abstract] [Full Text]
|
|
|
|
|
|
M. W. Wooten, M. L. Seibenhener, K. B. W. Neidigh, and M. L. Vandenplas Mapping of Atypical Protein Kinase C within the Nerve Growth Factor Signaling Cascade: Relationship to Differentiation and Survival of PC12 Cells Mol. Cell. Biol., July 1, 2000; 20(13): 4494 - 4504. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. C. Garcia, E. Banfi, and M. G. Pittis Infection of Macrophage-Like THP-1 Cells with Mycobacterium avium Results in a Decrease in Their Ability to Phosphorylate Nucleolin Infect. Immun., June 1, 2000; 68(6): 3121 - 3128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-p. Liu, S.-c. Hsiung, M. Adlersberg, T. Sacktor, M. D. Gershon, and H. Tamir Ca2+-Evoked Serotonin Secretion by Parafollicular Cells: Roles in Signal Transduction of Phosphatidylinositol 3'-kinase, and the gamma and zeta Isoforms of Protein Kinase C J. Neurosci., February 15, 2000; 20(4): 1365 - 1373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-G. Ying, P. Proost, J. van Damme, M. Bruschi, M. Introna, and J. Golay Nucleolin, a Novel Partner for the Myb Transcription Factor Family That Regulates Their Activity J. Biol. Chem., February 11, 2000; 275(6): 4152 - 4158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Martelli, G. Tabellini, R. Bortul, L. Manzoli, R. Bareggi, G. Baldini, V. Grill, M. Zweyer, P. Narducci, and L. Cocco Enhanced Nuclear Diacylglycerol Kinase Activity in Response to a Mitogenic Stimulation of Quiescent Swiss 3T3 Cells with Insulin-like Growth Factor I Cancer Res., February 1, 2000; 60(4): 815 - 821. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. M. NERI, A. M. MARTELLI, P. BORGATTI, M. L. COLAMUSSI, M. MARCHISIO, and S. CAPITANI Increase in nuclear phosphatidylinositol 3-kinase activity and phosphatidylinositol (3,4,5) trisphosphate synthesis precede PKC-{zeta} translocation to the nucleus of NGF-treated PC12 cells FASEB J, December 1, 1999; 13(15): 2299 - 2310. [Abstract] [Full Text]
|
|
|
|
|
|
M. Srivastava and H. B. Pollard Molecular dissection of nucleolin's role in growth and cell proliferation: new insights FASEB J, November 1, 1999; 13(14): 1911 - 1922. [Abstract] [Full Text]
|
|
|
|
|
|
P. J. Bates, J. B. Kahlon, S. D. Thomas, J. O. Trent, and D. M. Miller Antiproliferative Activity of G-rich Oligonucleotides Correlates with Protein Binding J. Biol. Chem., September 10, 1999; 274(37): 26369 - 26377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zhou, S. C. Lee, Z. Yao, and T.-H. Tan Hematopoietic Progenitor Kinase 1 Is a Component of Transforming Growth Factor beta -induced c-Jun N-terminal Kinase Signaling Cascade J. Biol. Chem., May 7, 1999; 274(19): 13133 - 13138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Tyson, J. T. Swarthout, and N. C. Partridge Increased Osteoblastic c-fos Expression by Parathyroid Hormone Requires Protein Kinase A Phosphorylation of the Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein at Serine 133 Endocrinology, March 1, 1999; 140(3): 1255 - 1261. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Tertrin-Clary, I. Eude, T. Fournier, B. Paris, M. Breuiller-Fouche, and F. Ferre Contribution of protein kinase C to ET-1-induced proliferation in human myometrial cells Am J Physiol Endocrinol Metab, March 1, 1999; 276(3): E503 - E511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.'i. Kuroda, N. Nakagawa, C. Tokunaga, K. Tatematsu, and K. Tanizawa Mammalian Homologue of the Caenorhabditis elegans UNC-76 Protein Involved in Axonal Outgrowth Is a Protein Kinase C zeta -interacting Protein J. Cell Biol., February 8, 1999; 144(3): 403 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Ginisty, H Sicard, B Roger, and P Bouvet Structure and functions of nucleolin J. Cell Sci., January 3, 1999; 112(6): 761 - 772. [Abstract] [PDF]
|
|
|
|
|
|
D. J. Burks, J. Wang, H. Towery, O. Ishibashi, D. Lowe, H. Riedel, and M. F. White IRS Pleckstrin Homology Domains Bind to Acidic Motifs in Proteins J. Biol. Chem., November 20, 1998; 273(47): 31061 - 31067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pederson Growth Factors in the Nucleolus? J. Cell Biol., October 19, 1998; 143(2): 279 - 281. [Full Text] [PDF]
|
|
|
|
|
|
P. C. Brandt and T. C. Vanaman Elevated Glucocorticoid Receptor Transactivation and Down-regulation of alpha 1 Integrin Are Associated with Loss of Plasma Membrane Ca2+-ATPase Isoform 1 J. Biol. Chem., August 4, 2000; 275(32): 24534 - 24539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. Ravichandran, D. L. Esposito, J. Chen, and M. J. Quon Protein Kinase C-zeta Phosphorylates Insulin Receptor Substrate-1 and Impairs Its Ability to Activate Phosphatidylinositol 3-Kinase in Response to Insulin J. Biol. Chem., January 26, 2001; 276(5): 3543 - 3549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhang, S.-L. Wu, and C. S. Rubin A Novel Adapter Protein Employs a Phosphotyrosine Binding Domain and Exceptionally Basic N-terminal Domains to Capture and Localize an Atypical Protein Kinase C. CHARACTERIZATION OF CAENORHABDITIS ELEGANS C KINASE ADAPTER 1, A PROTEIN THAT AVIDLY BINDS PROTEIN KINASE C3 J. Biol. Chem., March 23, 2001; 276(13): 10463 - 10475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhang, S.-L. Wu, and C. S. Rubin Structural Properties and Mechanisms That Govern Association of C Kinase Adapter 1 with Protein Kinase C3 and the Cell Periphery J. Biol. Chem., March 23, 2001; 276(13): 10476 - 10484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Perander, G. Bjorkoy, and T. Johansen Nuclear Import and Export Signals Enable Rapid Nucleocytoplasmic Shuttling of the Atypical Protein Kinase C lambda J. Biol. Chem., April 13, 2001; 276(16): 13015 - 13024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Leng, T. N. Chase, and M. C. Bennett Muscarinic Receptor Stimulation Induces Translocation of an alpha -Synuclein Oligomer from Plasma Membrane to a Light Vesicle Fraction in Cytoplasm J. Biol. Chem., July 20, 2001; 276(30): 28212 - 28218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bacqueville, P. Deleris, C. Mendre, M.-T. Pieraggi, H. Chap, G. Guillon, B. Perret, and M. Breton-Douillon Characterization of a G Protein-activated Phosphoinositide 3-Kinase in Vascular Smooth Muscle Cell Nuclei J. Biol. Chem., June 15, 2001; 276(25): 22170 - 22176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Sinclair and A. D. O'Brien Cell Surface-localized Nucleolin Is a Eukaryotic Receptor for the Adhesin Intimin-gamma of Enterohemorrhagic Escherichia coli O157:H7 J. Biol. Chem., January 18, 2002; 277(4): 2876 - 2885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mizukami, S. Kobayashi, F. Uberall, K. Hellbert, N. Kobayashi, and K.-i. Yoshida Nuclear Mitogen-activated Protein Kinase Activation by Protein Kinase Czeta during Reoxygenation after Ischemic Hypoxia J. Biol. Chem., June 23, 2000; 275(26): 19921 - 19927. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |