| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Volume 272, Number 51, Issue of December 19, 1997
pp. 32395-32400
(Received for publication, July 14, 1997, and in revised form, October 7, 1997)
From the Laboratory of Chemical Biology, NIDDK, National Institutes
of Health, Bethesda, Maryland 20892-1822
Erythropoietin (Epo) is known for its role in
erythropoiesis and acts by binding to its receptor (EpoR) on the
surface of erythroid progenitors. EpoR activity follows the site of
hematopoiesis from the embryonic yolk sac to the fetal liver and then
the adult spleen and bone marrow. Expression of EpoR has also been
observed in selected cells of non-hematopoietic origin, such as the
embryonic mouse brain during mid-gestation, at levels comparable to
adult bone marrow. EpoR transcripts in brain decrease during
development falling by birth to less than 1-3% of the level in
hematopoietic tissue. We have now recapitulated this pattern of
expression using a human EpoR transgene consisting of an 80-kb human
EpoR genomic fragment. The highest level of expression was observed in
the embryonic yolk sac and fetal liver, analogous to the endogenous gene, in addition to expression in adult spleen and bone marrow. Although activity of this transgene in brain is initially lower than
the endogenous gene, it does exhibit the down-regulation observed for
the endogenous gene in adult brain. The expression pattern of
hybrid transgenes of an hEpoR promoter fused to
Regulated Human Erythropoietin Receptor Expression in Mouse
Brain
-galactosidase in 9.5-day embryos suggested that the hEpoR promoter
region between 
1778 and 150 bp 5
of the transcription start site
is necessary to direct EpoR expression in the neural tube. EpoR
expression in the neural tube may be the origin of the EpoR transcripts
detected in brain during development. These data demonstrate that both the mouse and human EpoR genes contain regulatory elements to direct
significant levels of expression in a developmentally controlled manner
in brain and suggest that in addition to its function during erythropoiesis, EpoR may play a role in the development of selected non-hematopoietic tissue.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Milano and R. Collomp Erythropoietin and neuroprotection: a therapeutic perspective Journal of Oncology Pharmacy Practice, December 1, 2005; 11(4): 145 - 149. [Abstract] [PDF]
|
|
|
|
 |
|
 D. J. Foster, O. W. Moe, and C. C. W. Hsia Upregulation of erythropoietin receptor during postnatal and postpneumonectomy lung growth Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1107 - L1115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Suzuki, S. Imagawa, C. T. Noguchi, M. Yamamoto, and U. Klingmuller Do {beta}-globin, GATA-1,or EpoR regulatory domains specifically mark erythroid progenitors in transgenic reporter mice? Blood, November 1, 2004; 104(9): 2988 - 2989. [Full Text] [PDF]
|
|
|
|
 |
|
 H. H. Marti Erythropoietin and the hypoxic brain J. Exp. Biol., August 15, 2004; 207(18): 3233 - 3242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Heinrich, R. Pelanda, and U. Klingmuller A mouse model for visualization and conditional mutations in the erythroid lineage Blood, August 1, 2004; 104(3): 659 - 666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yu, J. J. Shacka, J. B. Eells, C. Suarez-Quian, R. M. Przygodzki, B. Beleslin-Cokic, C.-S. Lin, V. M. Nikodem, B. Hempstead, K. C. Flanders, et al. Erythropoietin receptor signalling is required for normal brain development Development, March 3, 2003; 129(2): 505 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ruscher, D. Freyer, M. Karsch, N. Isaev, D. Megow, B. Sawitzki, J. Priller, U. Dirnagl, and A. Meisel Erythropoietin Is a Paracrine Mediator of Ischemic Tolerance in the Brain: Evidence from an In Vitro Model J. Neurosci., December 1, 2002; 22(23): 10291 - 10301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yasuda, Y. Fujita, S. Masuda, T. Musha, K. Ueda, H. Tanaka, H. Fujita, T. Matsuo, M. Nagao, R. Sasaki, et al. Erythropoietin is involved in growth and angiogenesis in malignant tumours of female reproductive organs Carcinogenesis, November 1, 2002; 23(11): 1797 - 1805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Divoky, J. T. Prchal ;, X. Yu, and C. T. Noguchi Mouse surviving solely on human erythropoietin receptor (EpoR): model of human EpoR-linked disease Blood, May 15, 2002; 99(10): 3873 - 3875. [Full Text] [PDF]
|
|
|
|
|
|
T. Shingo, S. T. Sorokan, T. Shimazaki, and S. Weiss Erythropoietin Regulates the In Vitro and In Vivo Production of Neuronal Progenitors by Mammalian Forebrain Neural Stem Cells J. Neurosci., December 15, 2001; 21(24): 9733 - 9743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Yu, C.-S. Lin, F. Costantini, and C. T. Noguchi The human erythropoietin receptor gene rescues erythropoiesis and developmental defects in the erythropoietin receptor null mouse Blood, July 15, 2001; 98(2): 475 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Sasaki, S. Masuda, and M. Nagao Pleiotropic Functions and Tissue-Specific Expression of Erythropoietin Physiology, June 1, 2001; 16(3): 110 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Chikuma, S. Masuda, T. Kobayashi, M. Nagao, and R. Sasaki Tissue-specific regulation of erythropoietin production in the murine kidney, brain, and uterus Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1242 - E1248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Masuda, T. Kobayashi, M. Chikuma, M. Nagao, and R. Sasaki The oviduct produces erythropoietin in an estrogen- and oxygen-dependent manner Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1038 - E1044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. BICHET, R. H. WENGER, G. CAMENISCH, A. ROLFS, W. EHLEBEN, T. PORWOL, H. ACKER, J. FANDREY, C. BAUER, and M. GASSMANN Oxygen tension modulates ß-globin switching in embryoid bodies FASEB J, February 1, 1999; 13(2): 285 - 295. [Abstract] [Full Text]
|
|
|
|
|
|
H Wu, S. Lee, J Gao, X Liu, and M. Iruela-Arispe Inactivation of erythropoietin leads to defects in cardiac morphogenesis Development, January 8, 1999; 126(16): 3597 - 3605. [Abstract] [PDF]
|
|
|
|
 |
|
 Y. Yasuda, S. Masuda, M. Chikuma, K. Inoue, M. Nagao, and R. Sasaki Estrogen-dependent Production of Erythropoietin in Uterus and Its Implication in Uterine Angiogenesis J. Biol. Chem., September 25, 1998; 273(39): 25381 - 25387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ogilvie, X. Yu, V. Nicolas-Metral, S. M. Pulido, C. Liu, U. T. Ruegg, and C. T. Noguchi Erythropoietin Stimulates Proliferation and Interferes with Differentiation of Myoblasts J. Biol. Chem., December 8, 2000; 275(50): 39754 - 39761. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
